Identification of a newly isolated erythritol-producing yeast and cloning of its erythritol reductase genes

Abstract

A new erythritol-producing yeast (strain BH010) was isolated in this study. Analysis of the D1/D2 domain of the 26S rDNA sequence, the ITS/5.8S rDNA sequence, and the 18S rDNA sequence allowed the taxonomic position of strain BH010 to be discussed and it was identified and named Moniliella sp. BH010. Physiological characteristics were described. Scanning electron micrography clearly indicated that the cells were cylindrical to elliptical with an average size of 5 × 10 μm when growing in liquid medium, and that pseudohyphae and blastoconidia were observed when cultivated in agar plates. The erythritol reductase genes were cloned, sequenced, and analyzed. BLAST analysis and multiple sequence alignment demonstrated that erythritol reductase genes of Moniliella sp. BH010 shared very high homology with that of Trichosporonoides megachiliensis SNG-42 except for the presence of introns. The deduced amino acid sequences showed high homology to the aldo–keto reductase superfamily.

Polyol production during heterofermentative growth of the plant isolate Lactobacillus florum 2F

“…florum genome annotated as a 2,5‐diketo‐ d‐ gluconic acid reductase that shares amino acid similarity to fungal ERs (Deng et al . ). Similar to fungal ERs, 2,5‐diketo‐ d‐ gluconic acid reductases are aldo‐keto reductases that result in the oxidation of NAD(P)H (Chotani et al .…”

Erythritol production by yeasts: a snapshot of current knowledge

“…Genetic engineering gave promising results, but more information would be needed for further improvement. Although enzymes from the pentose phosphate pathway are usually well characterized, and ER genes had been identified in several organisms, the gene coding for erythrose-4-phopshate phosphatase has still not been identified in yeast (Carly, Vandermies, et al, 2017b;Deng, Han, Liu, Jia, & Zhou, 2012;Lee, Kim, et al, 2003a).…”

Erythritol as sweetener—wherefrom and whereto?

“…The needed high temperatures as well as the nickel catalyst result in a cost-ineffective reaction with a low product yield (Park et al 2005 ; Pfeifer et al 1960 ). When in 1950, traces of erythritol were found in the residue of Cuban blackstrap molasses fermented by yeast, a flourishing new possibility opened up: the biotechnological production of erythritol (Deng et al 2012 ). Please see Table 3 for an overview on the process parameters of the following discussed strategies or microorganisms.…”

“…Erythrose reductase is the key enzyme for the production of erythritol and a lot of research has been done on the characterization and purification of various reductases in yeasts (Deng et al 2012 ). In Trichoderma reesei , a filamentous fungus industrially used due to its ability to secrete vast amounts of cellulases and hemicellulases, the erythrose reductase is also naturally present and has already been characterized (Jovanovic et al 2013 ).…”