Erythritol production by yeasts: a snapshot of current knowledge

Carly, Frédéric; Fickers, Patrick

doi:10.1002/yea.3306

Cited by 41 publications

(28 citation statements)

References 89 publications

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“…Y. lipolytica produces polyols, mainly erythritol and mannitol. In the environment, Y. lipolytica produces erythritol in response to osmotic stress [1,97]. Metabolites derived from erythritol such as erythrulose and threitol will be also discussed in Section 3.1 to Section 3.3.…”

Section: Sugar Alcoholmentioning

confidence: 99%

“…EOL is not metabolized by the human body, and thus it is non-caloric and does not modify the glycemia index. EOL is also not cariogenic since it is not metabolized by bacteria responsible for dental caries [97]. In Y. lipolytica and other osmotolerant yeasts, EOL synthesis starts from erythrose-4P, an intermediate of the pentose phosphate pathway (PPP) ( Figure 2).…”

Section: Erythritolmentioning

confidence: 99%

“…EOL synthesis and production strategies in bioreactors have been recently reviewed by our groups and others [97,104,105]. Therefore, only the main achievements that are not covered in these reviews will be presented in this section.…”

Section: Erythritolmentioning

confidence: 99%

“…These authors also overexpress genes GND1 and ZWF1 in strain MK1 and obtained an EOL titer increased by 38% (40.2 g/L) and 41% (42.5 g/L). Non-directed mutagenesis such as NTG or UV mutagenesis has been also used in the past [97]. Beside this, atmospheric and room temperature plasma mutagenesis was also used to generate mutants of Y. lipolytica with increased EOL productivity.…”

Section: Erythritolmentioning

confidence: 99%

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Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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Section: Sugar Alcoholmentioning

confidence: 99%

Section: Erythritolmentioning

confidence: 99%

Section: Erythritolmentioning

confidence: 99%

Section: Erythritolmentioning

confidence: 99%

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Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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“…In recent studies a few genes involved in erythritol assimilation in Y. lipolytica have been identified, but the full pathway remains unknown ( Carly and Fickers, 2018 ; Regnat et al, 2018 ). Hitherto the known elements of the catabolic machinery are the transcriptional factor EUF1 (YALI0F01562g) ( Rzechonek et al, 2017 ), erythrulose kinase EYK1 (YALI0F01606g) ( Carly et al, 2017 ), and erythritol dehydrogenase EYD1 (YALI0F01650g) ( Carly et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

et al. 2018

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Erythritol is a natural sweetener produced by microorganisms as an osmoprotectant. It belongs to the group of polyols and it can be utilized by the oleaginous yeast Yarrowia lipolytica. Despite the recent identification of the transcription factor of erythritol utilization (EUF1), the metabolic pathway of erythritol catabolism remains unknown. In this study we identified a new gene, YALI0F01628g, involved in erythritol assimilation. In silico analysis showed that YALI0F01628g is a putative isomerase and it is localized in the same region as EUF1. qRT-PCR analysis of Y. lipolytica showed a significant increase in YALI0F01628g expression during growth on erythritol and after overexpression of EUF1. Moreover, the deletion strain ΔF01628 showed significantly impaired erythritol assimilation, whereas synthesis of erythritol remained unchanged. The results showed that YALI0F1628g is involved in erythritol assimilation; thus we named the gene EYI1. Moreover, we suggest the metabolic pathway of erythritol assimilation in yeast Y. lipolytica.

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In Yarrowia lipolytica erythritol catabolism ends with erythrose phosphate

Niang

Arias

Steels

et al. 2019

Cell Biology International

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In response to osmotic stress, the yeast Yarrowia lipolytica produces erythritol, a four‐carbon sugar alcohol, from erythrose‐P, an intermediate of the pentose phosphate pathway. Under non‐stressing conditions (isotonic environment), the produced erythritol is subsequently recycled into erythrose‐P that can feed the pentose phosphate pathway. Herein, gene YALI0F01584g was characterized as involved in the erythritol catabolic pathway. Several experimental evidences suggested that it encodes an erythrulose‐1P isomerase that converts erythrulose‐1P into erythrulose‐4P. On the basis of our previous reports and results gathered in this study with genetically modified strains, including ΔYALI0F01584g and ΔYALI0F01628g disrupted mutants, the entire erythritol catabolic pathway has been characterized.

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Erythritol production by yeasts: a snapshot of current knowledge

Cited by 41 publications

References 89 publications

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

A Role of a Newly Identified Isomerase From Yarrowia lipolytica in Erythritol Catabolism

In Yarrowia lipolytica erythritol catabolism ends with erythrose phosphate

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