Identification of a new proliferation‐associated protein NET‐1/C4.8 characteristic for a subset of high‐grade cervical intraepithelial neoplasia and cervical carcinomas

Abstract

A large number of genes are known to be differentially expressed at distinct steps of carcinogenesis. By using a cell culture model system for cervical cancer, we had previously identified several genes that were more strongly expressed when comparing normal cervical epithelium with cervical intraepithelial neoplasia (CIN) and cervical cancer. In our study, we show that one of these genes, C4.8, is identical to NET-1, which is a new member of the tetraspanin family of proteins. By generating a mouse polyclonal antiserum against the major extracellular domain of the protein, we could detect NET-1/C4.8 expression both after ectopic expression of the gene in cell cultures and in cryostat sections of cervical biopsies. Moreover, immunohistochemic analyses of normal cervical epithelium, metaplasia, condyloma and CIN of different severity suggest that NET-1/C4.8 expression is associated with neoplastic cell proliferation. Notably, expression of the protein throughout the entire epithelium is only evident for a subset of CIN3. The potential importance for this gene in cervical carcinogenesis is underlined by an invariably strong expression in all undifferentiated squamous cell cancers examined. This indicates that this gene may be of prognostic value.

Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

“…our data also showed that there was a significant correlation between the Tspan1 level and overall survival rate. Similarly, other reports show that there was a significant correlation between the Tspan1 expression and overall survival rate, disease stages as well as the pathological features of other various tumors, such as colorectal cancer and cervical carcinoma (13,23).…”

“…Tspan1, a new member of the tetraspanins group, was found to be elevated in some tumors (12,(23)(24)(25). Recent studies also suggested Tspan1 gene may play a role in the proliferation of cancer cells and be associated with cancer cell motility, implying a function of the gene in the development of various cancer (12,23,26). In this study, our results revealed that Tspan1 immunopositive staining was significantly correlated with the lymph node metastasis, pTNM stages and poor prognosis of PCC.…”

“…In many human cancers, tumor progression was found to be associated with an altered expression of tetraspanins (22). Tspan1, a new member of the tetraspanins group, was found to be elevated in some tumors (12,(23)(24)(25). Recent studies also suggested Tspan1 gene may play a role in the proliferation of cancer cells and be associated with cancer cell motility, implying a function of the gene in the development of various cancer (12,23,26).…”

Neuroepithelial transforming gene 1 functions as a potential prognostic marker for patients with non-small cell lung cancer

“…Multivariate analysis showed that Net-1 may be used as an independent prognostic biomarker for patients with NSCLC. Previous studies supports the notion that Net-1 is frequently overexpressed in various cancer types, including hepatocellular carcinoma, gastric cancer, gliomas, cervical carcinomas and breast cancer (10,(23)(24)(25)(26). Using IHC staining, Lahiff et al (14) found that Net-1 expression is markedly increased in invasive and metastatic adenocarcinoma of the oesophagogastric junction.…”

TSPAN1 protein expression: A significant prognostic indicator for patients with colorectal adenocarcinoma

“…TSPAN1 was reported as a tumor-related gene recently [13][14][15][16][17] . In several studies, TSPAN1 gene over-expression was detected in liver cancer [14] , prostate cancer [15] , gastric carcinoma [16] and cervix cancer [17] . It has been proposed that TSPAN1 plays a role in cell mitosis and/or cause cell abnormal differentiation.…”