Identification of a new plant extract for androgenic alopecia treatment using a non-radioactive human hair dermal papilla cell-based assay

Jain, Ruchy; Monthakantirat, Orawan; Tengamnuay, Parkpoom; De-Eknamkul, Wanchai

doi:10.1186/s12906-016-1004-5

Cited by 25 publications

(24 citation statements)

References 32 publications

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“…Many beauty products are currently available that incorporate C. asiatica extracts, such as cosmetic creams, hand and body lotions, eye gel, and face mask products (Bylka et al, 2014). A previous study found that C. asiatica extract enlarged hair follicles (Jain and Dass, 2015) and inhibited the activity of 5α-reductase that causes hair loss (Jain et al, 2016). However, few hair care or restoration products contain the extract, as its effect on the hair root remains unclear; the molecular mechanisms involved in plant extracts modulating gene expression are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Saansoomchai¹,

Limmongkon²,

Surangkul³

et al. 2018

CMUJNS

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Centella asiatica Linn. (C. asiatica) extract has been shown to possess high antioxidant activity due to its phenols and flavonoids. This study tested the efficacy of 70%-ethanol (EtOH) crude extracts of C. asiatica and its fractions (H 2 O, EtOAc, CH 2 Cl 2 , and hexane) to modulate human follicle dermal papilla cells. In addition, we analyzed the extracts for major phytochemicals as well as free radical scavenging activity. Our results from ABTS and DPPH assays showed that the amounts of phenolic and flavonoid compounds in the extracts were both related to its free radical scavenging activity. While the EtOAc fraction of C. asiatica demonstrated the highest free radical scavenging activity, it was toxic to human follicle dermal papilla cells. The cell viability test was positive when cells were treated with EtOH crude extract and H 2 O fraction. VEGF gene expression, quantified by real-time PCR analysis of the EtOH crude extract, showed a significant level of induction, indicating that the growth promotion effect in human follicle dermal papilla cells was related to VEGF gene expression, which has a positive hair growth stimulating effect. The EtOH crude extract of C. asiatica may offer potential in hair growth promoting products.

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Section: Introductionmentioning

confidence: 99%

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Saansoomchai¹,

Limmongkon²,

Surangkul³

et al. 2018

CMUJNS

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“…This finding was based on the use of a newly developed nonradioactive human hair dermal papilla cell-based assay, which directly detects dihydrotestosterone (DHT, 2), the enzymatic product, by high performance thin layer chromatography (HPTLC). 20,21) Even though the steroid 5α-reductase inhibitory activity of 5a was not as potent as dutasteride (4), the discovery of a new chemical scaffold has provided a potential for the development of a new group of steroid 5α-reductase inhibitors. Thus, we hypothesized that compounds having a furanonaphthoquinone, similar to 5a and a more simplified structure, naphthoquinone, would be used as the potential steroid 5α-reductase inhibitors.…”

mentioning

confidence: 99%

“…The steroid 5α-reductase inhibition was measured based on the amount of dihydrotestosterone (2) produced, determined using HPTLC as previously described. 20,21) Molecular docking using the homologous protein template of steroid 5α-reductase type 1 19) was performed via AutoDock Vina 25) to predict the biomolecular interaction between the 5α-reductase-reduced nicotinamide adenine dinucleotide phosphate (NADPH) binary complex and the respective furanonaphthoquinones. Herein, an essential pharmacophore and inhibitory activity of a potential steroid 5α-reductase inhibitor are discussed.…”

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confidence: 99%

Synthesis, Biological Evaluation and Molecular Docking of Avicequinone C Analogues as Potential Steroid 5α-Reductase Inhibitors

Karnsomwan

Netcharoensirisuk

Rungrotmongkol

et al. 2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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Avicequinone C (5a), a furanonaphthoquinone isolated from the Thai mangrove Avicennia marina has been shown previously to have interesting steroid 5α-reductase type 1 inhibitory activity. In this study, a series of avicequinone C analogues containing furanonaphthoquinone with different degrees of saturation and substituents at the furan ring were synthesized. The resulting synthetic avicequinone C and analogues (5a-f) along with some related compounds including 2,5-dihydroxy-1,4-benzoquinone (6) and natural naphthoquinones such as lawsone (7a) and lapachol (7b) were evaluated for their in vitro cell viability and steroid 5α-reductase type 1 inhibitory activities using the cultured cell line of human keratinocytes (HaCaT). This cell-based bioassay was performed based on a direct detection of the enzymatic product dihydrotestosterone (2) by using a non-radioactive high performance thin layer chromatography (HPTLC) method. Among the furanonaphthoquinones in this series, 5e having a propionic substituent at furan ring possessed approximately 22-fold more potent than the original isolated compound 5a. However, the compounds without furan motif such as 6, 7a and b could not inhibit the activity of steroid 5α-reductase. Molecular docking results of the in silico three-dimensional steroid 5α-reductase type 1-reduced nicotinamide adenine dinucleotide phosphate (NADPH) binary complex was performed via AutoDock Vina and it illustrated that the furanonaphthoquinone moiety and the substituent at furan ring might play a key role as pharmacophores for the steroid 5α-reductase inhibitory activity.

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“…Various methods are used to maintain the fertility and health of hair and minimalize and prevent hair problems. Health care for the hair can be done by way of cleaning your hair regularly and wear hair growth [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Characteristics and Alopecia Activity of Pakis Gajah (Angiopteris Evecta (G.FORST) Hoffm.) Growing in Galunggung Mountainside, West Java

Mustarichie

Ramdhani

Iskandar

2017

Asian J Pharm Clin Res

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Objective: This study aims to determine characteristics and alopecia activity of Pakis Gajah (Angiopteris evecta (G.Forst) Hoffm.) growing in Galunggung mountainside, West Java. Methods:A. evecta was collected, determined and extracted using ethanol 95% by maceration. The extract was characterized based on Farmakope Indonesia method. Phytochemical screening was applied based on Farnsworth's method. Its alopecia activity was determined by Tanaka method on male Angora rabbits. Results:The results showed that A. evecta simplicia had dried shrinkage characteristics of 3.35%; ash content 4.73%; water soluble sari content 20.11%; ethanol soluble concentration 5.94%; density 0.820; pH of extract 5.33; microchemical examination with H 2 SO 4 gives a dark brown color, with 2N NaOH giving a bright yellow color, with the alkaline solution giving fluorescence, with FeCl 3 giving a greenish-green color; its chemical contents were polyphenols, flavonoid, steroids, triterpenoids, quinones, monoterpenoid, and sesquiterpenoids. Alopecia activity showed that the water fraction and n-hexane had hair growth stimulating activity and water fraction was the fraction that had the most excellent activity, while ethyl acetate did not have such activities. The alopecia results were compared with minoxidil standards. Conclusion:These results indicated that the water fractions A. evecta had the most alopecia activity on research animal. Our results suggest before it can be used in humans, it is necessary to do further research on the bald volunteers.

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Identification of a new plant extract for androgenic alopecia treatment using a non-radioactive human hair dermal papilla cell-based assay

Cited by 25 publications

References 32 publications

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Synthesis, Biological Evaluation and Molecular Docking of Avicequinone C Analogues as Potential Steroid 5α-Reductase Inhibitors

Characteristics and Alopecia Activity of Pakis Gajah (Angiopteris Evecta (G.FORST) Hoffm.) Growing in Galunggung Mountainside, West Java

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