Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains

Zhu, Ling; Konsak, Barbara; Olaogun, Olusola Martins; Agnew-Crumptona, Rebecca; Kanci, Anna; Marenda, Marc S.; Browning, Glenn F.; Noormohammadi, Amir H.

doi:10.1016/j.vetmic.2017.08.021

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…A recent study [12] described 32 mutations within the MS-H genome as compared to its parent strain 86,079/ 7NS. However, the stability of these mutations after passage in vivo had only been tested for those found in obg [8] and oppF [9].…”

Section: Discussionmentioning

confidence: 99%

“…While the genetic basis of the MS-H temperature sensitivity and attenuation is not fully known yet, a mutation detected in obg gene was proposed as a likely explanation for the MS-H ts + phenotype [8]. Also, further comparison of the MS-H genome with that of its wild-type parent strain 86,079/7NS has revealed a frameshift mutation in an oligopeptide permease transporter (opp) gene, oppF 1 [9]. OppF is essential in establishment of systemic infection by M. bovis and its persistence in lower respiratory tract of calves [10].…”

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confidence: 99%

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Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

et al. 2020

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Background Genomic comparison of Mycoplasma synoviae vaccine strain MS-H and the MS-H parental strain 86,079/7NS established a preliminary profile of genes related to attenuation of MS-H. In this study we aimed to identify the stability of mutations found in MS-H after passage in experimental or field chickens, and to evaluate if any reverse mutation may be associated with changes in characteristics of MS-H in vitro or in vivo. Results Whole genome sequence analysis of 5 selected MS-H field reisolates revealed that out of 32 mutations reported previously in MS-H, 28 remained stable, while four found to be reversible to the wild-type. Each isolate possessed mutations in one to three of the genes obg, oppF1 and gap and/or a non-coding region. Examination of the 4 reversible mutations by protein modeling predicted that only two of them (in obg and oppF1 genes) could potentially restore the function of the respective protein to that of the wild-type. Conclusions These results suggest that the majority of the MS-H mutations are stable after passage in vaccinated chickens. Characterisation of stable mutations found in MS-H could be utilised to develop rapid diagnostic techniques for differentiation of vaccine from field strains or ts- MS-H reisolates.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

et al. 2020

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“…This would have time and cost advantages and represents an important area of future work. The direct use of the HRM assay on clinical samples has been successfully applied to several pathogens in the past [ 46 – 49 ]. The potential of HRM in identifying mixed specimens has been reported to be primarily dependent on the quantity and proportion of the target DNAs in the mixture [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny

et al. 2018

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Live attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombination events between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classify ILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generated after co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also to viruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification of ILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro . This method can be used to classify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understand recombination in these viruses. Electronic supplementary material The online version of this article (10.1007/s00705-018-4086-1) contains supplementary material, which is available to authorized users.

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“…Th is technique allows the presence of an adenine in a nucleotide at position 468 of the oppF-1 gene of the vaccine strain of M. synoviae-H. Indeed, the above-mentioned authors show the exclusivity of this mutation in the vaccine strain, compared to wild M. synoviae strains isolated in Australia (Zhu et al, 2017). Recently, diff erentiation between M. synoviae vaccine and fi eld strains was performed with indirect ELISA based on OppF-C gene (Kordafshari et al, 2019).…”

Section: Diagnosismentioning

confidence: 99%

Mycoplasma Synoviae Infection in Layers: Diagnosis and Control Measures – A Review

Kaboudi

Jbenyeni

2019

AVM

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Mycoplasmas are widespread bacteria in domestic and wild birds. Among the important species in laying hen, Mycoplasma gallisepticum and Mycoplasma synoviae, are considered as an emergent pathogen in the last few years worldwide, causing considerable economic losses as a result of falling eggs and the decrease in egg quality. Transmission of M. synoviae occurs horizontally, more rapidly in multi-age sites, and vertically, leading to a decline in hatchability in breeding farms. The interaction between M. synoviae and the host’s immune system explains the immunosuppression induced by this pathogen. Inside the cell, M. synoviae can escape the immune system by implementing several mechanisms.Subclinical respiratory infection is oft en associated to M. synoviae. However, severe disease may be observed in the presence of other factors (respiratory viruses, stressors). The emergence of a new form of clinical manifestation of disease associated to M. synoviae infection has been described since the 2000s. Eggshell apex abnormalities of the produced eggs, associated to high risk of cracks and breakage, is described.The diagnosis of M. synoviae infection is based on various tests, including serology, culture and biomolecular methods. Control is based on theacquisition of free mycoplasma birds, biosecurity, regular monitoring and vaccination. Management of other risk factors is essential.

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Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains

Cited by 14 publications

References 32 publications

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny

Mycoplasma Synoviae Infection in Layers: Diagnosis and Control Measures – A Review

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