Identification of a leaf-rust resistance gene in species of Aegilops L., synthetic forms, and introgression lines of common wheat

Davoyan, E. R.; Davoyan, R. O.; Bebyakina, I. V.; Davoyan, O. R.; Zubanova, Yu. S.; Kravchenko, A. M.; Зинченко, А.

doi:10.1134/s2079059712040041

Cited by 9 publications

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“…Aegilops L. genus has 5 fundamental genomes, which are C, D, M, S and U (D and S be called Balso). The genomes play an important role in the origin and evolution course of common wheat ( Davoyan et al, 2012 , Li et al, 2011 , Timonova et al, 2013 ). In our research, the material involves 4 species of Aegilops L genus, containing 5 chromosome groups.…”

Section: Discussionmentioning

confidence: 99%

Gene introgression from common wheat into Aegilops L.

Yuan

Cao

Ai-zhi

2017

Saudi Journal of Biological Sciences

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Group of experiments were carried out to verify possibility of gene introgression from common wheat into . The artificial indoor crossbreed was conducted using 7 genotypes from 4 wheat relative species as female, and common wheat as male. The experiment result shows that different species has variable cross ability. Among the 4 Aegilops species, the highest cross rate is from the combination of × (46.49% for genotype Ae42, 22.58% for Y92), the second is from × (14.76% for Y100, 12.11% for Ae23), the third is from × (2.23% for Ae7, 8.50% for Y145), and the lowest is from × (0.19%). Hybrid embryos from different combinations have different ability of callus initiation and germination. The hybrid embryos from/ and / have a higher level of callus initiation and germination. / has a middle level, while the has a lower level. The interspecific hybrids between and common wheat have so low fertility. In back-crosses, the seed-set rate of hybrids of / is 3.71% and 4.36% respectively back-crossed with male and female parents, while for hybrids of /, they were 0 and 0.33% respectively, and for /, 0.33% and 0 respectively. On selfing of the hybrids, the seed-set rate is 0 (no seed set from 9750 florets) for the combination of /, 0.044% (3 selfed seeds out of 6870 florets) for / and 0 (no seed set from 7253 florets) for /. The research suggests that the probability of gene introgression from into species is very low in nature.

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Section: Discussionmentioning

confidence: 99%

Gene introgression from common wheat into Aegilops L.

Yuan

Cao

Ai-zhi

2017

Saudi Journal of Biological Sciences

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“…Характеристи ка образцов Aegilops biuncialis по наличию амплифицированного фрагмента после ПЦР с праймерами J13 и ювенильной устойчивости к листовой ржавчине Urbanovich et al, 2006;Gajnullin et al, 2007;Gultyaeva et al, 2019), хотя достаточно очевидно, что амплификация фрагмента ДНК определенного размера вряд ли может рассматриваться как окончательное доказательство наличия гена устойчивости (вследствие рекомбинаций и мутаций в течение эволюции вида). В нескольких работах отечественных исследователей данный подход был применен для идентификации эффективных генов резистентности к листовой ржавчине и у образцов Aegilops (Dzhenin et al, 2009;Davoyan et al, 2012;Gultyaeva et al, 2014).…”

Section: Discussionunclassified

“…С помощью диагностических молекулярных маркеров, сцепленных с известными генами устойчивости к листовой ржавчине Lr9, Lr35 и Lr47, был проведен скрининг образцов ДНК диких сородичей, синтетических форм и интрогрессивных линий мягкой пшеницы (Davoyan et al, 2012). Ген Lr9 был идентифицирован в геноме всех изученных образцов вида Ae.…”

Section: Discussionunclassified

“…Метод ис пользования ПЦР-маркеров для идентификации генов устойчивости пшеницы и ее родичей, в том числе и представителей рода Aegilops, к листовой ржавчине в последнее время достаточно широко используется в нашей стране (Dzhenin et al, 2009;Davoyan et al, 2012;Gultyaeva et al, 2014).…”

Section: использование молекулярно-генетического и фитопатологического методов для идентификации генов эффективной устойчивости к листовоunclassified

“…Данные праймеры ранее уже были использованы для идентификации этих генов резистентности у образцов рода Aegilops (например, Dzhenin et al, 2009;Davoyan et al, 2012).…”

Section: материалы и методыunclassified

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The use of molecular-genetic and phytopathological methods to identify genes for effective leaf rust resistance in Aegilops accessions

Тырышкин

Kolesova

2020

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Identification of effective genes for disease resistance in resistant plant samples is the most important step toward recommending them for breeding. There are three main methods for such identification: hybridological analysis, phytopathological test, and DNA marking. The method of PCR markers is widely used in Russia to identify resistance genes in wheat relatives, including the genus Aegi lops L. for resistance to leaf rust. From a theoretical viewpoint, the presence of a certain amplification fragment can hardly be interpreted as a definite proof of the presence of a resistance gene: during the species evolution, recombinations and mutations could occur, resulting in disturbance of the fragment’s presence and phenotypic expression of its connection with resistance. The purpose of this work was a comparison between molecular-genetic and phytopathological methods to identify leaf rust resistance genes Lr9 and Lr41 in three Aegilops species.Materials and methods. We identified leaf rust resistance genes Lr9 and Lr41 in forty Aegilops accessions using PCR with J13 and GDM35 primers, respectively. In the phytopathological test, the seedlings were infected with the pathogen population (avirulent to Lr9 and Lr41 genes) and the fungus clones virulent to the wheat line with the Lr9 gene.Results and conclusions. According to the data of molecular marking, the Lr41 gene was present in twelve Ae. tauschii Coss. accessions; Lr9 in four Ae umbellulata Zhuk. accessions and four of Ae. biuncialis Vis. All accessions of Ae. tauschii, two of Ae. umbellulata, and three of Ae. biuncialis, possessing effective resistance genes according to the molecular testing, were susceptible to the pathogen population. For three Ae. umbellulata accessions resistant to the population, where DNA marking failed to identify an Lr9 gene, the presence of this gene was shown by a phytopathological test. Thus, there were significant differences in the postulation of effective Lr9 and Lr41 leaf rust resistance genes in Aegilops accessions after a phytopatological test and the use of DNA markers.

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Phenotypic and genetic diversity of leaf rust resistance in wheat wild relatives

Sadeghabad

Dadkhodaie

Heidari

2020

Journal of Phytopathology

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Puccinia triticina (Pt), the causal agent of leaf rust evolves through forming new pathotypes that adversely affect the growth and yield of wheat cultivars. Therefore, continued production of resistant varieties through exploring novel sources of resistance in wild relatives which are abundantly found in Iran and the neighbouring regions is a major task in wheat breeding programs. The aim of the present study was to explore 60 wild wheat genotypes selected from the species Triticum monococcum, Aegilops tauschii, Ae. neglecta, Ae. cylindrica, Ae. triuncialis, Ae. umbellulata, Ae. speltoides, Ae. columnaris, Ae. crassa and Ae. ventricosa for resistance to leaf rust. The cultivar ‘Boolani’ and Thatcher near‐isogenic lines were used as controls. Two‐week‐old seedlings were inoculated using 10 Pt pathotypes, and the infection types were recorded. The genotypes were also analysed for polymorphism using six sequence‐tagged sites (STS) and sequence characterized amplified region (SCAR) markers. Forty‐eight genotypes produced high infection types (3+) for two pathotypes, but the remaining genotypes produced low infection types of ‘0; =’ to ‘1+CN’ to all pathotypes. The latter included three accessions of Ae. tauschii, two accessions of each Ae. umbellulata, Ae. columnaris and Triticum monococcum, and one accession from each Ae. triuncialis, Ae. ventricosa and Ae. neglecta. Analysis for STS and SCAR markers suggested several genotypes could carry the genes Lr9, Lr10, Lr19, Lr24, Lr26 and Lr37 or their potential orthologs in addition to unknown resistance genes. In conclusion, the identified resistant genotypes could be further characterized and used in wheat breeding programs for leaf rust resistance.

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Identification of a leaf-rust resistance gene in species of Aegilops L., synthetic forms, and introgression lines of common wheat

Cited by 9 publications

References 5 publications

Gene introgression from common wheat into Aegilops L.

Gene introgression from common wheat into Aegilops L.

The use of molecular-genetic and phytopathological methods to identify genes for effective leaf rust resistance in Aegilops accessions

Phenotypic and genetic diversity of leaf rust resistance in wheat wild relatives

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