Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development

Gomes, Dayane Santos; Silva, Edson Mário de Andrade; Rosa, Emilly Caroline de Andrade; Gualberto, Nina Gabriela Silva; Souza, Monaliza Átila de Jesus; Santos, Gesivaldo; Pirovani, Carlos Priminho; Micheli, Fabienne

doi:10.1016/j.fgb.2021.103635

Cited by 3 publications

(10 citation statements)

References 79 publications

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“…Two proteomic studies of the necrotrophic phase of the mycelium were identified. One compared the S and C biotypes [ 20 ] and the other compared six developmental stages of the fungus according to mycelium color and development (white, yellow, pink, dark pink; primordium and basidiocarp) [ 23 ]. In the first study, proteins involved in virulence, pathogenicity and stress response were identified abundantly in all cultures [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the second one, proteins related to IAA metabolism, cell proliferation and cytoskeleton activity were identified most abundantly in the white mycelium phase, suggesting they are required for mycelial development. In the dark pink phase, proteins were identified related to vesicular transport processes mediated by small GTPase signaling, cell wall biogenesis, stress response and response to nutrient deficiency, which were also associated with the primordium phase [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

“…Since then, several other studies have been published analyzing how biotrophy is maintained for so long in M. perniciosa , what the molecular mechanisms involved in the necrotrophic phase are, and the participation of hosts in signaling the end of these fungal phases [ 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Biochemical modifications of hosts when infected by M. perniciosa have also been investigated [ 27 ]. Cultivation protocols have been established, such as the artificial cultivation in cookies that allow the production of basidiocarps [ 28 ], followed by molecular studies of the fungus’ necrotrophic phase based on this type of cultivation [ 23 , 29 ]. With technological advances, improvements in the cultivation and manipulation techniques of the fungus have been achieved [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…The challenge of in vitro production of the fungus in the biotrophic phase has been overcome [ 9 ]. Fungal protein profiles have been traced and key proteins related to pathogenicity and necrosis have been identified [ 21 , 22 , 23 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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Significant scientific advances to elucidate the Moniliophthora perniciosa pathosystem have been achieved in recent years, but the molecular biology of this pathogen-host interaction is still a field with many unanswered questions. In order to present insights at the molecular level, we present the first systematic review on the theme. All told, 1118 studies were extracted from public databases. Of these, 109 were eligible for the review, based on the inclusion and exclusion criteria. The results indicated that understanding the transition from the biotrophic-necrotrophic phase of the fungus is crucial for control of the disease. Proteins with strong biotechnological potential or that can be targets for pathosystem intervention were identified, but studies regarding possible applications are still limited. The studies identified revealed important genes in the M. perniciosa-host interaction and efficient molecular markers in the search for genetic variability and sources of resistance, with Theobroma cacao being the most common host. An arsenal of effectors already identified and not explored in the pathosystem were highlighted. This systematic review contributes to the understanding of the pathosystem at the molecular level, offering new insights and proposing different paths for the development of new strategies to control witches’ broom disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Santos

Mora-Ocampo

Novais

et al. 2023

IJMS

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Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

Zugaib

Gramacho

Mares

et al. 2023

Journal of Phytopathology

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Moniliasis caused by the fungus Moniliophthora roreri is one of the most destructive cacao diseases in tropical America. In 2021, the presence of the pathogen was confirmed in cacao plantations in Brazil. This is the first proteomic study of the M. roreri protein profile of ungerminated spores and mycelium compared by 2D SDS‐PAGE (Two‐dimensional gel electrophoresis) associated with mass spectrometry. A total of 446 spots were detected on ungerminated spores gels and 402 spots on M. roreri mycelial gels. A total of 29 proteins were identified from the ungerminated spores, 21 of them were exclusive, and 53 were identified in the mycelium, 16 of them were exclusive. Most of the identified proteins at both development stages were categorized as being involved in metabolic processes, reduction/oxidation processes, and protein synthesis and folding. The interaction networks observed expand the known interactions of these proteins, resulting in eight functional clusters for proteins identified in ungerminated spores and seven clusters for mycelial proteins. In addition, a biological model of ungerminated spores and mycelium with cell location of proteins was constructed. The results contribute to a better understanding of the metabolic mechanisms of M. roreri during dormancy and vegetative development, which can support future studies for strategies to control moniliasis.

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Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

et al. 2023

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A set of diseases caused by fungi and oomycetes are responsible for large losses in annual world cocoa production. Managing the impact caused by these diseases is very complex because a common solution has yet to be found for different pathogens. In this context, the systematic knowledge of Theobroma cacao L. pathogens’ molecular characteristics may help researchers understand the possibilities and limitations of cocoa disease management strategies. This work systematically organized and summarized the main findings of omics studies of T. cacao eukaryotic pathogens, focusing on the plant–pathogen interaction and production dynamics. Using the PRISMA protocol and a semiautomated process, we selected papers from the Scopus and Web of Science databases and collected data from the selected papers. From the initial 3169 studies, 149 were selected. The first author’s affiliations were mostly from two countries, Brazil (55%) and the USA (22%). The most frequent genera were Moniliophthora (105 studies), Phytophthora (59 studies) and Ceratocystis (13 studies). The systematic review database includes papers reporting the whole-genome sequence from six cocoa pathogens and evidence of some necrosis-inducing-like proteins, which are common in T. cacao pathogen genomes. This review contributes to the knowledge about T. cacao diseases, providing an integrated discussion of T. cacao pathogens’ molecular characteristics, common mechanisms of pathogenicity and how this knowledge is produced worldwide.

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Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development

Cited by 3 publications

References 79 publications

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

State of the Art of the Molecular Biology of the Interaction between Cocoa and Witches’ Broom Disease: A Systematic Review

Comparative proteome profile of ungerminated spores and mycelium of the fungus Moniliophthora roreri, causal agent of frosty pod rot disease in cacao

Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

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