Identification of a human T lymphocyte surface protein associated with the E-rosette receptor.

Kamoun, Malek; Martin, Paul J.; Hansen, John A.; Brown, Melissa A.; Siadak, Anthony W.; Nowinski, Robert C.

doi:10.1084/jem.153.1.207

Cited by 377 publications

(70 citation statements)

References 20 publications

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“…In some experiments, PBMC were fractionated into E + and E-cell fractions by rosetting with 2-aminoethylisothiouronium bromide-treated sheep erythrocytes (33) and reconstituted at the indicated E+:E-cell ratios. T cells and NK cells both surface express CD2, which binds to sheep erythrocytes (34)(35)(36)(37), so the E + cell fractions predominantly contained T cells and NK cells.…”

Section: Patients and Controlsmentioning

confidence: 99%

Impaired recovery and cytolytic function of CD56+T and non—T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease‐discordant twins

Stohl¹,

Elliott²,

Hamilton³

et al. 1996

Arthritis & Rheumatism

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Objective. To determine whether there is impaired generation and cytolytic function of CD56+ T cells and non-T cells in human systemic lupus erythematosus (SLE).Methods. Peripheral blood mononuclear cells (PBMC) were obtained from 73 patients with SLE, 39 normal controls, and 9 pairs of monozygotic (MZ) twins discordant for SLE. PBMC were stimulated with anti-CD3 monoclonal antibody, maintained in interleukin-2, and assayed for percentages of total CD56+ cells and CD56+ T cells by flow cytometry, and for cytolytic activity against "Cr-labeled Daudi target cells.Results. Despite normal total cell expansion, the percentages of recovered CD56+ T cells and total CD56+ cells were 1.6-fold and 1.8-fold lower, respectively, in patients with SLE compared with normal controls (P = 0.011 and P < 0.001, respectively).Cytolytic activities of isolated total CD56+ cells and CD56+ T cells and were also reduced in patients with SLE compared with normal controls (P = 0.033). These defects associated with SLE were independent of disease activity and immunosuppressive medications, and they reflected impaired maturation of cytolytic effector cells rather than a deficiency in precursor cell number. In Submitted for publication March 29, 1996; accepted in revised form May 22, 1996. MZ twins discordant for SLE, recovered percentages of CD56+ cells and cytolytic responses were very low in 4 of 8 and 6 of 9 co-twins with SLE, respectively. Cellmixing experiments with the PBMC of the MZ twins demonstrated that the E+ cell fractions (containing all T cells and CD56+ non-T cells) from the co-twins with SLE had decreased ability to generate cytolytic activity compared with the corresponding E+ cell fractions from the healthy co-twins. However, recovered percentages of CD56+ cells and non-T cells and cytolytic responses were also depressed in 4 of 8 and 4 of 9 healthy co-twins, respectively.

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Section: Patients and Controlsmentioning

confidence: 99%

Impaired recovery and cytolytic function of CD56+T and non—T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease‐discordant twins

Stohl¹,

Elliott²,

Hamilton³

et al. 1996

Arthritis & Rheumatism

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“…It was first described as the sheep erythrocyte receptor because it induces T and NK cells to form rosettes upon incubation with sheep red cells (5). Engagement of the CD2 antigen with its ligands induces T-cell activation and proliferation (6).…”

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confidence: 99%

CD2 is expressed by a subpopulation of normal B cells and is frequently present in mature B‐cell neoplasms

et al. 2002

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“…The current experiments demonstrated that the expression of the CD2 molecule, i.e., the E-rosette receptor (13,15,17,43), was elevated 10-to 20-fold on human T lymphocytes after activation. The elevation occurred on T cells that were activated either with PHA, which may directly interact with CD2, or with antibody to the CD3-Ti complex (Figs.…”

Section: Discussionmentioning

confidence: 58%

“…The E-rosette receptor (CD2) (13,15,17,43) is a differentiation antigen that occurs on virtually all T cells, whereas CD4 and CD8 identify T cell subsets (31). In addition to these differentiation antigens, there are also activation antigens that are expressed only on stimulated cells.…”

mentioning

confidence: 99%

Simultaneous increased expression of E‐rosette receptor (CD2, T11) and T cell growth factor receptor on human T lymphocytes during activation

Redelman¹

1987

Cytometry

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The E-rosette receptor (CD2, T11) is a differentiation antigen expressed on immature and mature human T lymphocytes. Activation of T cells from human peripheral blood with phytohemagglutinin (FHA) or with monoclonal antibody to the 0 3 -T i complex (anti-Leu-4) caused the expression of 0 2 to increase 10-to 20-fold. Dual parameter correlated analyses with antibody to the T cell growth factor ('I'CGF) receptor (anti-Tac) and anti-CD2 antibody demonstrated that the increase in CD2 expression occurred at the same time and on the same cells that expressed the TCGF receptor after stimulation with PHA. The increased expression of CD2 and the initial expression of Tac were totally inhibited by cycloheximide, but were not affected by sufficient actinomycin-D to block the T cell proliferative response. The expression of CD2 was compared with the expression of CD4 and CD8, i.e., T cell differentiation antigens on cytotoxic/suppressor or helper T cells, respectively. Although virtually all of the small percentage of freshly isolated Tac+ peripheral blood cells belonged to the CD4+, CD8-subset, both CD4+ and CD8+ T cells were equivalently activated by PHA to express Tac. By 20-30 hr after activation, the expression of CD4 or CD8 was initially decreased 10-50%. Subsequently, the expression of CD4 and CD8 returned to the levels on resting T cells but did not increase further. Therefore, the increase in CD2 expression does not reflect a universal property of cell surface antigens on activated T lymphocytes.Key terms: E-rosette receptor (CD2, T11); T cell growth factor or Interleukin-2 ('I'CGF, IL-2); Tar antigen; T cell differentiation antigens CD3-Ti, CD4, CD8; Monoclonal antibodies to Leu-Za, Leu-3a, Leu-4, Leu-5b, T11T cell precursors undergo differentiation and maturation in the thymus to be released as immunocompetent T cells that are found in lymph nodes and peripheral blood, These mature T cells express unique surface antigens that allow phenotypic identification of lymphocytes and lymphocyte subsets (3). The E-rosette receptor (CD2) (13,15,17,43) is a differentiation antigen that occurs on virtually all T cells, whereas CD4 and CD8 identify T cell subsets (31). In addition to these differentiation antigens, there are also activation antigens that are expressed only on stimulated cells. The receptor (Tac antigen) (45) for T cell growth factor (TCGF, also known as Interleukin-2, IL-2) is one such T cell activation antigen. The current data show that CD2 is an activation antigen as well, since its expression is increased by 10-to 20-fold on activated T cells.The initiation of a n immune response involves the stimulation of CD4+ and CD8+ T lymphocytes that ultimately express helper or cytotoxic/suppressor functions, respectively. TCGF is a polypeptide growth hormone initially produced early in T cell activation (38,44). It is essential for the proliferation of T cells stimulated with specific antigens or with polyclonal activators, e.g., antibody to the CD3-Ti complex (18,42) or lectins such as concanavalin-A (Con-A) or ph...

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Identification of a human T lymphocyte surface protein associated with the E-rosette receptor.

Cited by 377 publications

References 20 publications

Impaired recovery and cytolytic function of CD56+T and non—T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease‐discordant twins

Impaired recovery and cytolytic function of CD56+T and non—T cells in systemic lupus erythematosus following in vitro polyclonal T cell stimulation. Studies in unselected patients and monozygotic disease‐discordant twins

CD2 is expressed by a subpopulation of normal B cells and is frequently present in mature B‐cell neoplasms

Simultaneous increased expression of E‐rosette receptor (CD2, T11) and T cell growth factor receptor on human T lymphocytes during activation

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