Identification of a human ribosomal protein mRNA with increased expression in colorectal tumours

Chester, Kerry; Robson, L; Begent, R. H. J.; Talbot, Ian C.; Pringle, James H.; Primrose, L; Macpherson, Andrew J.; Boxer, G. M.; Southall, Peter; Malcolm, Alan D. B.

doi:10.1016/0167-4781(89)90119-x

Cited by 57 publications

(30 citation statements)

References 20 publications

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“…Reports have also been published that observed the altered expression of some ribosomal proteins in human cancers (21)(22)(23)(24)(25). The present study is the first to compare L13 mRNA expression levels in tumor versus normal tissue; it demonstrated that the level of L13 mRNA was higher in 28% of gastric, 41% of colorectal and 20% of liver cancer tissues.…”

Section: Discussionmentioning

confidence: 62%

“…For example, the gene encoding ribosomal protein S19 is mutated in approximately 25% of patients with DiamondBlackfan anemia, which is a rare congenital erythroblastopenia (20). Also, a differential expression of specific ribosomal protein genes was reported earlier as general characteristics of various tumor cell types (21)(22)(23)(24)(25).…”

Section: Introductionmentioning

confidence: 99%

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Activation of the ribosomal protein L13 gene in human gastrointestinal cancer

Kobayashi

Sasaki²,

Oshima³

et al. 2006

Int J Mol Med

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Abstract. Although ribosomal proteins are major components of ribosomes, recent data have shown them to have extraribosomal functions apart from ribosome and protein biosynthesis. In our earlier study, we showed that ribosomal protein L13 mRNA was up-regulated in response to DNA damage in hamster cells. We report here that L13 expression is up-regulated in human gastrointestinal cancers. We also examined the biological role of L13 on human cancer cells. Knocking down L13 expression using small interfering RNA (siRNA) resulted in drastic attenuation of cancer cell growth with significant G1 and G2/M arrest of the cell cycle. Moreover, L13 siRNA significantly enhanced the cellular sensitivity to certain DNA damaging agents and, concordantly, L13-overexpressing cells demonstrated greater chemoresistance compared to parent cells, suggesting an inverse correlation between L13 expression and chemosensitivity. By using semiquantitative RT-PCR, we analyzed expression of L13 in freshly resected cancer tissue of the stomach, colorectum and liver. Up-regulation of L13 mRNA expression was observed in 10 (28%) of 36 gastric, 19 (41%) of 46 colorectal and 5 (20%) of 25 liver cancer tissue samples compared to adjacent normal tissue samples. We also found that increased expression of the L13 gene correlated with clinical staging in gastric cancers. The results of this study suggest that L13 plays an essential role in the progression of some gastrointestinal malignancies.

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Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Activation of the ribosomal protein L13 gene in human gastrointestinal cancer

Kobayashi

Sasaki²,

Oshima³

et al. 2006

Int J Mol Med

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“…A sequence equivalent to 10 amino acids preceding the initiation codon AUG in the sequences of clone 12, the cDNA clone from colon cDNA library [331, and LLRep3 is identical to the corresponding region of SUP44. Furthermore, another AUG initiation codon that immediately precedes these 10 amino acids is present in clone 12 and the cDNA clone from colon cDNA library [33] Overexpression of another human ribosomal protein, L31, in 23 of 23 colorectal tumors has also been reported [47].Theexpressionof L31 gene, which has72% homology with the protein L44 from the large ribosomal subunit of yeast, increases coordinately fivefold to sixfold during estrogen-stimulated cell growth [@I. Pogue-Geile et al have shown that other ribosomal proteins, i.e., S6,58, S12, L5, and PO, are overexpressed in colon cancers and adenomatous polyps compared with normal mucosa 1491.…”

Section: Discussionmentioning

confidence: 93%

Elevated expression of the ribosomal protein S2 gene in human tumors

Chiao

Shin

Sacks

et al. 1992

Molecular Carcinogenesis

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Differential screening of a cDNA library was used to isolate genes differentially expressed in a nontumorigenic clone and a ras-transformed variant of the human teratocarcinoma cell line PA-1. The RNA transcript for one of the cDNA clones that we identified was expressed at a 25-fold higher level in the ras-transformed PA-1 cells than in the nontumorigenic PA-1 cells. DNA sequence analysis of this clone showed that it had 86% nucleic acid homology to the mouse LLRep3 gene and only differed at a single amino acid codon (codon 198), which is changed from serine in LLRep3 to threonine in this cDNA clone. The rat ribosomal S2 protein is closely related to the yeast omnipotent informational suppressor SUP44, which encodes the yeast ribosomal protein S4; to the mouse protein LLRep3; and to the human cDNA clone we describe in this report. We therefore concluded that this clone codes for the human ribosomal S2 protein. In situ hybridization experiments revealed that expression of this gene was elevated in cultured human head and neck squamous cell carcinomas compared with normal keratinocytes. In situ hybridization experiments also demonstrated that expression of this gene was elevated in histological sections of human premalignant leukoplakia, head and neck squamous cell carcinomas, and colon and breast cancers compared with the adjacent normal tissues. S2 expression may be a useful diagnostic or prognostic marker for grading human tumors.

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“…The mRNA was found overexpressed in all 23 CRC by slot-blot hybridization. In situ hybridization results illustrated that RPL31 was more abundant in CRC than normal colonic epithelium or stromal tissues suggesting its role in proliferation and neoplasia [16]. In 1991, Northern blot analysis showed RPS3, S6, S8, S12, L5, and P0 increased in CRC and adenomatous polyps except RPL26 and RPL35.…”

Section: Ribosomal Proteins and Colorectal Car-cinomamentioning

confidence: 97%

Ribosomal Proteins and Colorectal Cancer

Lai

Xu²

2007

123

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Abstract:The ribosome is essential for protein synthesis. The composition and structure of ribosomes from several organisms have been determined, and it is well documented that ribosomal RNAs (rRNAs) and ribosomal proteins (RPs) constitute this important organelle. Many RPs also fill various roles that are independent of protein biosynthesis, called extraribosomal functions. These functions include DNA replication, transcription and repair, RNA splicing and modification, cell growth and proliferation, regulation of apoptosis and development, and cellular transformation. Previous investigations have revealed that RP regulation in colorectal carcinomas (CRC) differs from that found in colorectal adenoma or normal mucosa, with some RPs being up-regulated while others are down-regulated. The expression patterns of RPs are associated with the differentiation, progression or metastasis of CRC. Additionally, the recent literature has shown that the perturbation of specific RPs may promote certain genetic diseases and tumorigenesis. Because of the implications of RPs in disease, especially malignancy, our review sought to address several questions. Why do expression levels or categories of RPs differ in different diseases, most notably in CRC? Is this a cause or consequence of the diseases? What are their possible roles in the diseases? We review the known extraribosomal functions of RPs and associated changes in colorectal cancer and attempt to clarify the possible roles of RPs in colonic malignancy.

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Identification of a human ribosomal protein mRNA with increased expression in colorectal tumours

Cited by 57 publications

References 20 publications

Activation of the ribosomal protein L13 gene in human gastrointestinal cancer

Activation of the ribosomal protein L13 gene in human gastrointestinal cancer

Elevated expression of the ribosomal protein S2 gene in human tumors

Ribosomal Proteins and Colorectal Cancer

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