Identification of a gene on human chromosome 8q11 that is differentially expressed during prostate-cancer progression

Chang, Glenn T.G.; Tapsi, Nita; Steenbeek, Martine; Blok, Leen J.; Weerden, Wytske M. van; Alewijk, Dirk C.J.G. van; Eussen, Bert; Steenbrugge, G.J. van; Brinkmann, Albert O.

doi:10.1002/(sici)1097-0215(19991112)83:4<506::aid-ijc12>3.0.co;2-0

Cited by 20 publications

(34 citation statements)

References 15 publications

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“…Clone JC19 was isolated as a transcript expressed at a five-fold higher level in an androgen-dependent prostate cancer cell line (LNCaP-FGC), as compared to three androgen-independent prostate cancer cell lines (LNCaP-LNO, PC3 and DU145). Furthermore, using a panel of androgendependent and androgen-independent human prostate cancer xenografts, a similar change in JC19 expression was observed (Chang et al, 1997). Clone JC19 was identified to encode REPS2 (RALBP1-associated Eps domain containing protein 2).…”

Section: Introductionmentioning

confidence: 62%

“…As indicated in the Introduction, REPS2 expression is significantly reduced in androgen-independent prostate cancer (Chang et al, 1997;Oosterhoff et al, 2003). Based on these findings, and in view of indications that REPS2 is involved in growth factor signaling (Nakashima et al, 1999), it was hypothesized that REPS2 might play a role in the transition of prostate cancer from androgen-dependent towards androgen-independent growth.…”

Section: Discussionmentioning

confidence: 95%

“…However, remaining prostate cancer cells progress from androgen-dependent towards androgen-independent growth, within a few years, and this means transition to uncontrollable cancer. To study molecular and cellular mechanisms involved in this transition, we have conducted a differential display PCR between androgendependent and androgen-independent prostate cancer cells (Chang et al, 1997). Clone JC19 was isolated as a transcript expressed at a five-fold higher level in an androgen-dependent prostate cancer cell line (LNCaP-FGC), as compared to three androgen-independent prostate cancer cell lines (LNCaP-LNO, PC3 and DU145).…”

Section: Introductionmentioning

confidence: 99%

“…Although REPS2 is differentially expressed between androgen-dependent and androgen-independent prostate cancer cell lines, it is not androgen regulated (Chang et al, 1997). Furthermore, the REPS2 gene is located on the human X chromosome at Xp22, and transcribes into a single mRNA of approximately 7000 base pairs.…”

Section: Introductionmentioning

confidence: 99%

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Identification of REPS2 as a putative modulator of NF-κB activity in prostate cancer cells

2004

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The protein REPS2 is implicated in growth factor receptor-mediated endocytosis and signalling, and its expression is downregulated in androgen-independent prostate cancer cells. Herein, the NF-jB subunit p65 is identified as a human REPS2 protein partner, interacting with the EH domain of REPS2. Using crystal structure data from literature and experimental data from yeast and mammalian two-hybrid analysis, the results indicate that the NPF-motif in p65 acts as binding site for the EH domain in REPS2. However, in cultured prostate cancer cells, the REPS2-p65 interaction is triggered upon stimulation with phorbol ester (PMA). This indicates that PMA-sensitive signalling pathways can affect the interaction between REPS2 and p65. During prostate cancer progression from androgen-dependent to androgen-independent growth, downregulation of REPS2 is accompanied by upregulation of NF-jB activity. This might involve loss of REPS2-p65 interaction, which would lead to increased NF-jB activity. Androgen-deprivation causes apoptosis of prostate cancer cells, and activated NF-jB is a known inhibitor of apoptosis. Hence, decreased expression of REPS2 might be a key factor, causing prostate cancer cells to become resistant to induction of apoptosis by androgen deprivation.

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Section: Introductionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of REPS2 as a putative modulator of NF-κB activity in prostate cancer cells

2004

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“…A widely used approach has been the analysis of expression patterns by di erential display (Liang and Pardee, 1992), which has allowed the identi®cation of genes and proteins that could be relevant for these processes, such as PTI-1 (Shen et al, 1995), tropomyosin, a subunit of cytochrome c (Wang et al, 1996), ®bronectin, UEV-1 (Stubbs et al, 1999), or previously unknown genes (Blok et al, 1995;Chang et al, 1997;Lucas et al, 1998;Ronidelli and Tricoll, 1999). More recently, di erential hybridization on cDNA microarrays has been used for similar purposes (Lin et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks

et al. 2001

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In a search for molecular markers of progression in prostate cancer by means of di erential display, we have identi®ed a new gene, which we have designated PTOV1. Semiquantitative RT ± PCR has established that nine out of 11 tumors overexpress PTOV1 at levels signi®cantly higher than benign prostatic hyperplasia or normal prostate tissue. The human PTOV1 protein consists almost entirely of two repeated blocks of homology of 151 and 147 amino acids, joined by a short linker peptide, and is encoded by a 12-exon gene localized in chromosome 19q13.3. A Drosophila melanogaster PTOV1 homolog also contains two tandemly arranged PTOV blocks. A second gene, PTOV2, was identi®ed in humans and Drosophila, coding for proteins with a single PTOV homology block and unrelated amino-and carboxyl-terminal extensions. A 1.8-Kb PTOV1 transcript was detected abundantly in normal human brain, heart, skeletal muscle, kidney and liver, and at low levels in normal prostate. Immunocytochemical analysis and expression of chimeric GFP-PTOV1 proteins in cultured cells showed a predominantly perinuclear localization of PTOV1. In normal prostate tissue and in prostate adenomas, PTOV1 was undetectable or expressed at low levels, whereas nine out of 11 prostate adenocarcinomas showed a strong immunoreactivity, with a focal distribution in areas of carcinoma and prostatic intraepithelial neoplasia. Therefore, PTOV1 is a previously unknown gene, overexpressed in early and late stages of prostate cancer. The PTOV homology block represents a new class of conserved sequence blocks present in human, rodent and¯y proteins. Oncogene (2001) 20, 1455 ± 1464.

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Use of nude mouse xenograft models in prostate cancer research

2000

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Identification of a gene on human chromosome 8q11 that is differentially expressed during prostate-cancer progression

Cited by 20 publications

References 15 publications

Identification of REPS2 as a putative modulator of NF-κB activity in prostate cancer cells

Identification of REPS2 as a putative modulator of NF-κB activity in prostate cancer cells

PTOV1, a novel protein overexpressed in prostate cancer containing a new class of protein homology blocks

Use of nude mouse xenograft models in prostate cancer research

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