Use of nude mouse xenograft models in prostate cancer research

Weerden, Wytske M. van; Romijn, Johannes C.

doi:10.1002/1097-0045(20000601)43:4<263::aid-pros5>3.0.co;2-i

Cited by 118 publications

(54 citation statements)

References 28 publications

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“…The time required to perform xenotransplant studies in mice ranges from several weeks to months. Moreover, it has been difficult to generate mouse xenotransplant models that exhibit tumor metastasis [21].…”

Section: The Mouse Model For Cancer Researchmentioning

confidence: 99%

Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish

et al. 2006

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In this research, we optimized parameters for xenotransplanting WM-266-4, a metastatic melanoma cell line, including zebrafish site and stage for transplantation, number of cells, injection method, and zebrafish incubation temperature. Melanoma cells proliferated, migrated and formed masses in vivo. We transplanted two additional cancer cell lines, SW620, a colorectal cancer cell line, and FG CAS/Crk, a pancreatic cancer cell line and these human cancers also formed masses in zebrafish. We also transplanted CCD-1092Sk, a human fibroblast cell line established from normal foreskin and this cell line migrated, but did not proliferate or form masses. We quantified the number of proliferating melanoma and normal skin fibroblasts by dissociating xenotransplant zebrafish, dispensing an aliquot of CM-DiI labeled human cells from each zebrafish onto a hemocytometer slide and then visually counting the number of fluorescently labeled cancer cells. Since zebrafish are transparent until approximately 30 dpf, the interaction of labeled melanoma cells and zebrafish endothelial cells (EC) can be visualized by whole-mount immunochemical staining. After staining with Phy-V, a mouse anti-zebrafish monoclonal antibody (mAb) that specifically labels activated EC and angioblasts, using immunohistology and 2-photon microscopy, we observed activated zebrafish EC embedded in human melanoma cell masses. The zebrafish model offers a rapid efficient approach for assessing human cancer cells at various stages of tumorigenesis.

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Section: The Mouse Model For Cancer Researchmentioning

confidence: 99%

Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish

et al. 2006

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“…Several researchers have developed human PCa xenograft models by transplanting clinical specimens of human PCa or cultured human PCa cells into immunodeficient mice [143]. Using these animal models investigators have attempted to validate the in vitro observations on the growth-inhibitory actions of vitamin D compounds in PCa cells [35e45].…”

Section: Studies In Animal Modelsmentioning

confidence: 99%

Vitamin D and Prostate Cancer

Krishnan¹,

Feldman²

2011

Vitamin D

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“…The PC346C cells, established from a human primary tumor via xenograft [45,46] , form an androgen-dependent prostate cancer cell line expressing Wt AR and CK8/ 18 [31] . After long-term androgen ablation, another androgen-independent subline, PC346DCC, with a 95% decrease in AR expression was generated from PC346C cells.…”

Section: Ar Dual Roles In Human Prostate Cancer Cellsmentioning

confidence: 99%

Androgen receptor roles in benign and malignant prostate disease

Wang

Shang

Niu

2011

Clin. Oncol. Cancer Res.

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Androgen deprivation therapy (ADT), which aims to reduce androgen-androgen receptor (AR) signaling, is the normal method of prostate cancer treatment. Despite its early success in suppressing prostate tumor growth, the therapy eventually fails, leading to recurrent hormone-refractory tumor growth. Recent studies have been carried out with stromal cell-specific or fibroblastspecific AR knockout mice or prostate stromal-specific and epithelial-specific AR knockout transgenic mice prostate cancer models and in vitro and in vivo studies of various human prostate cancer cells with knock-in and knock-out of the AR. These have indicated that the AR in prostatic stroma acts as a proliferation stimulator and survival factor, whereas epithelial AR acts as a survival factor for epithelial luminal cells and stromal smooth muscle cell differentiation, and as a suppressor for epithelial basal intermediate cell proliferation. These two opposite roles of the stromal and epithelial AR pose a major challenge for ADT and should be taken into account when developing new therapies targeting AR in selective cells.

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Use of nude mouse xenograft models in prostate cancer research

Cited by 118 publications

References 28 publications

Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish

Human melanoma cells transplanted into zebrafish proliferate, migrate, produce melanin, form masses and stimulate angiogenesis in zebrafish

Vitamin D and Prostate Cancer

Androgen receptor roles in benign and malignant prostate disease

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