Identification of a gene, ccr-1 (sll1242), required for chill-light tolerance and growth at 15 °C in Synechocystis sp. PCC 6803

Yin, Chuntao; Li, Weizhi; Du, Yiqing; Kong, Renqiu; Xu, Xudong

doi:10.1099/mic.0.2006/005074-0

Cited by 24 publications

(41 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Synechocystis, chill-light tolerance is rapidly increased within 24 h of preconditioning (26). ccr1 (sll1242, previously called ccr-1) is a gene required for growth at 15°C (27). When Synechocystis cells are directly transferred from 30°C to a chill (5°C)-light stress, the gene is also required for the ability to reinitiate growth.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

et al. 2011

View full text Add to dashboard Cite

). Hypothetically, certain factors induced during preconditioning are involved in acquisition of chill-light tolerance. In this study, Rbp1 (RNA-binding protein 1) rather than Rbp2 was found to be accumulated during preconditioning, and the accumulation of Rbp1 was correlated with the increase of chill-light tolerance. Inactivation of its encoding gene rbp1 led to a great reduction in the acquired chill-light tolerance, while ectopic expression of rbp1 enabled the cyanobacterium to survive the chill-light stress without preconditioning. Microarray analyses suggested that the Rbp1-dependent chill-light tolerance may not be based on its influence on mRNA abundance of certain genes. Similarly to that in Synechocystis, the Rbp1 homologue(s) can be accumulated in Microcystis cells collected from a subtropic lake in low-temperature seasons. Rbp1 is the first factor shown to be both accumulated early during preconditioning and directly involved in development of chill-light tolerance in Synechocystis. Its accumulation may greatly enhance the overwintering capability in certain groups of cyanobacteria.

show abstract

mentioning

confidence: 99%

“…Measurements of the ability to reinitiate growth (ARG), the relative acquired chill-light tolerance (RACLT), and fatty acid composition were performed as previously described (26,27). For growth of transformants, kanamycin (Km), erythromycin (Em), or spectinomycin (Sp) was added to the medium at 30 g ml…”

mentioning

confidence: 99%

Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Yin 等通过对 PCC6803 的研究发现, 该藻株对低温光照条件敏感, 不利于藻株存活 [17] ; Yang 等进一步研究发现藻株内 α-维生素 E 含量增加对其存活率提升有较大影响 [18] ; 而 Wan 等的最新研究结果揭示了微囊藻在为期 31d 的底泥环境中, 显示出了一定的抗逆性, 且 PS II 活性能较快恢复 [19] . 泊富营养化调查规范》 (第二版) [20] ; 同时使用血球计数板对微囊藻细胞进行计数, 微囊藻细胞净复苏率定义为每天装置内收集到的微囊藻细胞数除以装置的截面积(相当于底泥面积).…”

Section: 长在世界范围内也属罕见其水华优势种一年中大部分时间主要为铜绿微囊藻(Microcystis Aeruginosa)unclassified

Simulation on the sediments affecting <i>Microcystis</i> recruitment in north bay of Lake Dianchi

Wan

Tang²,

Лин³

et al. 2009

Journal of Lake Sciences

View full text Add to dashboard Cite

Fubao Bay is located in the north of Lake Dianchi, which is one of the most seriously polluted bays in the lake. Two main rivers, Haihe River and Daqinghe River flow into Fubao Bay from the north. Four sampling sites were settled in this bay, and the sediment samples were collected by using Petersen grab. For the first time, the study of recruitment ability of Microcystis in different sediment environments was carried out in simulation devices. The results in the present study showed that the recruitment ability of Microcystis was quite different depended on its habitat environments. The Microcystis recovery was inhibited in lacustrine sediments near estuary, and the maximum biomass (calculated as chlorophyll-a) was pretty lower than other sampling sites. Compared among East Coast Area, West Coast Area and Hydraulic Mud Fill Area, the Microcystis biomass were only 4.7%, 6.6% and 11.9%, respectively. And Microcystis biomass (calculated as Microcystis cell numbers) were about 5.2%, 10.3% and 19.4%, corresponding.All the results indicated that sediments in estuary of Fubao Bay were not appropriate for Microcystis recruitment. The contribution of "seeds bank" recruiting to form HABs in this bay could be much less than the wind-induced external loading. All these results could be helpful in Microcystis blooms forecasting, preventing and controlling in the future.

show abstract

“…PCC 6803 from J. Zhao at Institute of Hydrobiology (CAS) was cultured in BG11 mixotrophically at 30°C in the light of 30 μE m − 2 s − 1 as previously described (Yin et al, 2007). Spectinomycin at 10 μg ml − 1 was added to the medium as needed.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp. NJ-18

Wang

2012

Gene

View full text Add to dashboard Cite

Identification of a gene, ccr-1 (sll1242), required for chill-light tolerance and growth at 15 °C in Synechocystis sp. PCC 6803

Cited by 24 publications

References 26 publications

Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

Simulation on the sediments affecting <i>Microcystis</i> recruitment in north bay of Lake Dianchi

Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp. NJ-18

Contact Info

Product

Resources

About