Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

Tan, Xiaoming; Zhu, Tao; Shen, Shuai; Yin, Chuntao; Gao, Hong; Xu, Xudong

doi:10.1128/jb.01454-10

Cited by 25 publications

(43 citation statements)

References 24 publications

(43 reference statements)

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“…Total P (TP) and dissolved inorganic P (DIP) in the lake were determined each time the samples were collected, using the molybdenum blue colorimetric method (Murphy and Riley 1962). Single colonies of typical morphotypes were isolated by light microscopy and purified on a series of BG-11 (Rippka et al 1979) agar plates until the cultures were axenic, as verified by sequencing of the 16S rRNA gene (Davis et al 2009) and gvpA-gvpC intergenic sequences (Tan et al 2011). These colonies were then maintained aseptically in BG-11 medium at 25±1°C at a continuous irradiance of 30 μmol photons m −2 s −1…”

Section: Methodsmentioning

confidence: 99%

“…The significant bloom in the summer of 2007 affected Wuxi City and its local residents, thereby prompting urgent resolution of current environmental problems by preventing and controlling blue-green algal blooms (Liu et al 2011b). Field investigations and other studies showed that the dominant species Microcystis flosaquae, Microcystis aeruginosa, Microcystis wesenbergii, and Microcystis ichthyoblabe represent 85.7 to 90 % of Microcystis (Tan et al 2009;Liu et al 2011a;Otten and Paerl 2011;Tan et al 2011). M. flos-aquae is the predominant species among different long-term sampling sites in May, June, July, and November, whereas M. wesenbergii is predominant between August and October (Chen et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Comparative studies on phosphate utilization of two bloom-forming Microcystis spp. (cyanobacteria) isolated from Lake Taihu (China)

Tao

Zhang

2013

J Appl Phycol

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Microcystis is a common freshwater bloomdominating cyanobacterial genus. However, the crucial factors that affect the seasonal succession and extent of dominance of different species remain largely unknown. This study investigated inorganic phosphorus (P) uptake, growth, and P utilization of two dominant Microcystis species from Lake Taihu (China) at different P concentrations and temperatures. Compared with Microcystis wesenbergii, Microcystis flosaquae had higher maximum uptake rate and luxury storage coefficient. However, M. flos-aquae also had a higher P demand for growth. Under P-rich conditions (64.5 to 174.5 μM), M. flos-aquae had a higher growth rate (0.16 to 0.21 d ) at 22 to 30°C. M. wesenbergii, with higher phosphate affinity, had a lower P demand to sustain its growth, and yielded a higher growth rate of 0.10 d −1 at low P concentration (6.5 μM) at 34°C, whereas M. flos-aquae had a lower growth rate of 0.03 d −1. Therefore, M. flos-aquae was dominant in late spring under P-rich conditions, whereas M. wesenbergii prevailed in hot summer after M. flos-aquae decreased under P-limited conditions. These results agree well with their succession patterns in the field. Overall, succession and dominance of M. flos-aquae and M. wesenbergii in Lake Taihu are regulated by P concentration and water temperature.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative studies on phosphate utilization of two bloom-forming Microcystis spp. (cyanobacteria) isolated from Lake Taihu (China)

Tao

Zhang

2013

J Appl Phycol

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“…The DNA extraction protocol was based on previously described procedures for extracting total DNA from lake cyanobacterial samples [31]. A volume of 15 mL of cyanobacterial cells in the exponential phase was collected by centrifugation, washed with 10 mL of TE (10 mmol/L Tris.Cl, 1 mmol/L EDTA, pH 8.0) and then resuspended in 1 mL of TE.…”

Section: Dna Extractionmentioning

confidence: 99%

Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Liu¹,

Zhu²,

Lü³

et al. 2013

Applied Energy

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“…In previous reports, we showed that ␣-tocopherol, which is membrane localized, is essential for the acquired chill-light tolerance (ACLT) of cyanobacteria (7) and that accumulation of RNAbinding protein 1 (Rbp1) leads to the formation of overwintering capability of cyanobacteria (8,12). Nonetheless, information about the biology of overwintering cyanobacteria is still very limited.…”

mentioning

confidence: 99%

“…Studies on how cyanobacteria adapt to low temperatures can be roughly divided into two categories: (i) cold acclimation of cells exposed to a suboptimal growth temperature, such as 22 to 24°C (9,10) or 15°C (11) and (ii) survival under long-term (8 to 10 days) chill (5°C)-light stress as a simulation of overwintering (7,8,12). In previous reports, we showed that ␣-tocopherol, which is membrane localized, is essential for the acquired chill-light tolerance (ACLT) of cyanobacteria (7) and that accumulation of RNAbinding protein 1 (Rbp1) leads to the formation of overwintering capability of cyanobacteria (8,12).…”

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Role of Spermidine in Overwintering of Cyanobacteria

Zhu

Yin

et al. 2015

J Bacteriol

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Polyamines are found in all groups of cyanobacteria, but their role in environmental adaptation has been barely investigated. In Synechocystis sp. strain PCC 6803, inactivation of spermidine synthesis genes significantly reduced the survivability under chill (5°C)-light stress, and the survivability could be restored by addition of spermidine. To analyze the effects of spermidine on gene expression at 5°C, lacZ was expressed from the promoter of carboxy(nor)spermidine decarboxylase gene (CASDC) in Synechocystis. Synechocystis 6803::P CASDC -lacZ pretreated at 15°C showed a high level of LacZ activity for a long period of time at 5°C; without the pretreatment or with protein synthesis inhibited at 5°C, the enzyme activity gradually decreased. In a spermidine-minus mutant harboring P CASDC -lacZ, lacZ showed an expression pattern as if protein synthesis were inhibited at 5°C, even though the stability of its mRNA increased. Four other genes, including rpoA that encodes the ␣ subunit of RNA polymerase, showed similar expression patterns. The chill-light stress led to a rapid increase of protein carbonylation in Synechocystis. The protein carbonylation then quickly returned to the background level in the wild type but continued to slowly increase in the spermidine-minus mutant. Our results indicate that spermidine promotes gene expression and replacement of damaged proteins in cyanobacteria under the chill-light stress in winter. IMPORTANCEOutbreak of cyanobacterial blooms in freshwater lakes is a worldwide environmental problem. In the annual cycle of bloomforming cyanobacteria, overwintering is the least understood stage. Survival of Synechocystis sp. strain PCC 6803 under longterm chill (5°C)-light stress has been established as a model for molecular studies on overwintering of cyanobacteria. Here, we show that spermidine, the most common polyamine in cyanobacteria, promotes the survivability of Synechocystis under longterm chill-light stress and that the physiological function is based on its effects on gene expression and recovery from protein damage. This is the first report on the role of polyamines in survival of overwintering cyanobacteria. We also analyzed spermidine synthesis pathways in cyanobacteria on the basis of bioinformatic and experimental data.

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Role of Rbp1 in the Acquired Chill-Light Tolerance of Cyanobacteria

Cited by 25 publications

References 24 publications

Comparative studies on phosphate utilization of two bloom-forming Microcystis spp. (cyanobacteria) isolated from Lake Taihu (China)

Comparative studies on phosphate utilization of two bloom-forming Microcystis spp. (cyanobacteria) isolated from Lake Taihu (China)

Hydrocarbon profiles and phylogenetic analyses of diversified cyanobacterial species

Role of Spermidine in Overwintering of Cyanobacteria

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