Identification of a differential expression signature associated with tumorigenesis and metastasis of laryngeal carcinoma

Çoşkunpınar, Ender; Oltulu, Yasemin Müşteri; Orhan, Kadir Serkan; Tiryakioglu, Necip Ozan; Kanliada, Deniz; Akbaş, Fahri

doi:10.1016/j.gene.2013.10.063

Cited by 11 publications

(11 citation statements)

References 49 publications

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“…Caspase-3 is called the death protease because it is a critical executive protease whose activation leads inevitably to apoptosis [25,26]. The C-Myc transcription factor is found constitutively expressed in many cancers; including lung, stomach, breast and colon cancer and also in cervical carcinoma [27][28][29][30]. C-Myc stimulates the expression of many genes and it promotes cell proliferation and inhibits apoptosis [31] [32].…”

Section: Discussionmentioning

confidence: 99%

Over-expression of MEOX2 promotes apoptosis through inhibiting the PI3K/Akt pathway in laryngeal cancer cells

Tian

Tao

et al. 2018

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The early-stage diagnosis and treatment for the recurrence of larynx carcinoma needs further investigation. Mesenchyme homeobox 2 (MEOX2) was speculated as a novel suppressor gene in larynx carcinoma in our study, the molecular mechanism was studied. Real-time quantitative PCR (RT-qPCR) and Western blot were used to detect mRNA and protein levels of MEOX2 in laryngeal cancer tissues and cells (Hep-2, TU212, AMC-NH-8 and TU686 cells), and also apoptosis and phosphoinositide 3-kinase (PI3K)/protein kinase (Akt) related factors in TU212 cells transfected with MEOX2. Cell counting kit-8 (CCK8) assay and Annexin-Ⅴ/PI staining assay were conducted to determine cell viability and apoptosis rates respectively.46 patients with larynx carcinoma were involved in this study. The expression of MEOX2 was lower in larynx carcinoma tissues than normal tissues, correlated with clinical stages, differentiated degrees, and survival times. The expression of MEOX2 was the lowest among those laryngeal cancer cells, and was chosen to be transfected with MEOX2 in the following study. Over-expression of MEOX2 inhibited cell viability and promoted apoptosis of TU212 cells, via increasing the expression levels of Caspase-3, and decreasing levels of C-Myc, XIAP, PI3K p110α, PI3K p110β, PI3K class III and p-Akt. In summary, the expression levels of MEOX2 were inhibited in larynx carcinoma than normal tissues, correlated with the progression of the cancer. Over-expression of MEOX2 in laryngeal cancer cells inhibited cell viability and promoted apoptosis, via regulating apoptosis and PI3K/Akt pathway related factors. It would provide evidence for MEOX2 to be used as a therapeutical gene in larynx carcinoma.

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Section: Discussionmentioning

confidence: 99%

Over-expression of MEOX2 promotes apoptosis through inhibiting the PI3K/Akt pathway in laryngeal cancer cells

Tian

Tao

et al. 2018

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“…In addition, human papillomavirus (HPV) infections have been suggested to play a significant role in the progression of laryngeal cancer [16,40]. There is some evidence that positive family history of laryngeal cancer or other HNSCC (head and neck squamous cell carcinoma) may increase the risk of LCSS development [3,32]. The male/female ratio for the incidence of LCSS is significantly higher than in other tumours in this group [32].…”

Section: Introductionmentioning

confidence: 99%

New miRNA expression abnormalities in laryngeal squamous cell carcinoma

Cybula

Wieteska

Józefowicz-Korczyńska

et al. 2016

CBM

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Abstract. BACKGROUND:Although the development of novel diagnostic and treatment strategies concerning laryngeal cancer is highly intensive, the survival rate remains virtually unchanged. Small non-coding RNAs appear to be very promising biomarkers -and so remain the focus of extensive investigation in laryngeal cancer. OBJECTIVE: We examined the expression of five miRNA and five genes related to cancer whether they could be potential laryngeal cancer biomarkers. METHODS:We performed an analysis in 47 patients diagnosed with laryngeal cancer. The qPCR technique was used to investigate the expression profile. RESULTS: While miR-21-3p and miR-525-5p were found to be significantly up-regulated, miR-139-3p and miR-885-5p expression is lower in laryngeal cancer. Moreover, PIK3R1 and HACE1 were found to be also down-regulated. CONCLUSIONS: The change in miRNA expression is frequent than the expression of other tested genes. The expression of passenger strands such as miR-21-3p and miR-139-3p, which are rarely investigated, is also significantly affected in laryngeal cancer. While PIK3R1, HACE1, miR-139-3p, and miR-885-5p may act as tumor suppressor genes in the studied tumour type, miR-21-3p and miR-525-5p seem to have oncogenic properties. Our findings suggest that miR-885-5p and PIK3R1 are the best indicators for the classification of laryngeal cancer tissue and normal mucosa.

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“…12,18,[20][21][22]28,29 Simultaneous analysis of gene expression levels in the tumor and surgical margins has rarely been performed. 23,26,30 Cancer development is the result of the accumulation of genetic and epigenetic alterations. Because changes in certain genes occur in the very early stages of tumorigenesis, the detection of preneoplastic alterations in the surgical margin could facilitate the detection of cancer.…”

Section: Discussionmentioning

confidence: 99%

“…9 Coskunpinar et al analyzed the expression levels of 88 genes in laryngeal carcinoma using a PCR array and showed an altered expression of 16 genes when compared to paired cancer-free tissue. 30 One of the altered genes in which expression was significantly higher in the tumor tissue was the TIMP1 gene. TIMP1 protein expression was significantly higher in laryngeal squamous cell carcinoma than in adjacent non-cancerous tissues.…”

Section: Timp3mentioning

confidence: 99%

Expression profiles of selected genes in tumors and matched surgical margins in oral cavity cancer: Do we have to pay attention to the molecular analysis of the surgical margins?

Strzelczyk¹,

Krakowczyk²,

Gołąbek³

et al. 2018

Adv Clin Exp Med

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Identification of a differential expression signature associated with tumorigenesis and metastasis of laryngeal carcinoma

Cited by 11 publications

References 49 publications

Over-expression of MEOX2 promotes apoptosis through inhibiting the PI3K/Akt pathway in laryngeal cancer cells

Over-expression of MEOX2 promotes apoptosis through inhibiting the PI3K/Akt pathway in laryngeal cancer cells

New miRNA expression abnormalities in laryngeal squamous cell carcinoma

Expression profiles of selected genes in tumors and matched surgical margins in oral cavity cancer: Do we have to pay attention to the molecular analysis of the surgical margins?

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