Identification of a cytochrome P450 hydroxylase, CYP81A6, as the candidate for the bentazon and sulfonylurea herbicide resistance gene, Bel, in rice

Leaf proteome analysis provides insights into the molecular mechanisms of bentazon detoxification in rice

“…Rice develops resistance to this herbicide due to the ability of bentazon to induce expression of the P450 gene CYP81A6. Sensitivity of CYP81A6 mutants to bentazon further confirmed that CYP81A6 is a key mediator of bentazon resistance [8,9]. It has also been shown that unknown protein factor(s) can rapidly induce CYP81A6 transcription within 2 h of bentazon exposure [10].…”

Generation of tribenuron‐methyl herbicide‐resistant OsCYP81A6‐expressing rapeseed (Brassica napus L.) plants for hybrid seed production using chemical‐induced male sterility

“…, Zhang et al. ). To investigate the response to bentazon in the OsCYP81A6 ‐expressing transgenic rapeseed plants, T 1 plants were sprayed with 0, 0.06, 0.12, 1.6, 4.8 and 6.0 g/l of bentazon.…”

“…Oilseed rape hybrids display heterosis and CIMS systems are used to exploit this heterosis (Guan andStringam 1998, Yu et al 2006). Previous studies have demonstrated that expression of OsCYP81A6 confers resistance to bentazon and sulphonylurea herbicides in rice, Arabidopsis, tobacco and cotton through herbicide detoxification (Pan et al 2006, Zhang et al 2007, Ke et al 2012). In the present study, expression of OsCYP81A6 was also found to confer resistance to both bentazon and TBM in rapeseed plants (Figs 2,3 and 5 (m) Wild-type plants and T 2 generation CYP81A6 #11 transgenic plants were sprayed with 5 mg/l of TBM.…”

“…The OsCYP81A6 has been also identified to confer resistance to bentazon in rice (Pan et al 2006, Zhang et al 2007). To investi-gate the response to bentazon in the OsCYP81A6-expressing transgenic rapeseed plants, T 1 plants were sprayed with 0, 0.06, 0.12, 1.6, 4.8 and 6.0 g/l of bentazon.…”

The other, ignored HIV — highly invasive vegetation

“…In other words, a crop (rice) is transgenically modified to make it highly susceptible to a herbicide that otherwise is not harmful to the crop. The gene of choice CYP81A6 encodes a natural resistance to the herbicide bentazon in rice (Zhang et al 2007). Using the RNAi-type technology described in the section on "Molecular solutions to Striga", rice plants susceptible to bentazon were generated by suppressing the expression of the detoxification gene.…”