Identification of a copper-transporting ATPase involved in biosynthesis of A. flavus conidial pigment

Chang, Perng‐Kuang; Scharfenstein, Leslie L.; Mack, Brian M.; Wei, Qijian; Gilbert, Matthew K.; Lebar, Matthew D.; Cary, Jeffrey W.

doi:10.1007/s00253-019-09820-0

Cited by 20 publications

(15 citation statements)

References 41 publications

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“…Thirteen A. flavus and eleven A. oryzae genome sequences (Table ) were retrieved from the National Center for Biotechnology Information (NCBI) genome database (https://www.ncbi.nlm.nih.gov/pubmed). In this study, toxigenic A. flavus isolates CA14 (Chang et al ), NRRL3357 (Nierman et al ), CS1137, CS0504 and CS05040 (Ohkura et al ) are referred to as L‐type, and toxigenic isolates AF12, AZS and AF70 are referred to as S‐type (Ohkura et al ). Isolates WRRL1519 (Yin et al ) and NRRL35739 (TX13‐5) are atoxigenic L‐type A. flavus.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide nucleotide variation distinguishesAspergillus flavusfromAspergillus oryzaeand helps to reveal origins of atoxigenicA. flavusbiocontrol strains

Chang

2019

J Appl Microbiol

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Aims: To use genome-wide single nucleotide polymorphisms (total SNPs) to develop a molecular method for distinguishing Aspergillus flavus and Aspergillus oryzae. Methods and Results: Thirteen A. flavus and eleven A. oryzae genome sequences were obtained from the National Center for Biotechnology Information. These sequences were analysed by Mauve, a multiple-genome alignment program, to extract total SNPs between isolates of A. flavus, A. oryzae, or the two species. Averages of total SNPs of A. flavus isolates belonging to the same sclerotial morphotype (L-type = 178 952 AE 14 033; S-type = 133 188 AE 16 430) and A. oryzae isolates (152 336 AE 49 124) were consistently lower than those between the morphotypes and between the two species. Averages of total SNPs for L-type vs S-type (300 116 AE 1562) and S-type A. flavus vs A. oryzae (301 797 AE 4123) were similar but were 36% greater than that of L-type A. flavus vs A. oryzae (226 240 AE 10 779). Based on the devised criterion, ATCC 12892, Aspergillus oryzae (Ahlburg) Cohn, which had an averaged total SNPs 10-fold greater than that of other A. oryzae isolates, was determined to be close to Aspergillus parasiticus. Atoxigenic A. flavus field isolates, WRRL1519 and NRRL35739, were shown to more closely resemble A. oryzae than toxigenic L-type A. flavus. Biocontrol strains AF36 and K49 were genetically close to toxigenic L-type A. flavus. NRRL21882, the active agent of the commercialized biocontrol product Afla-Guard â GR, was genetically distant from all other A. flavus isolates. Conclusions: The close genetic relatedness between A. flavus and A. oryzae was confirmed and the evolutionary origins of atoxigenic A. flavus biocontrol strains were revealed. Significance and Impact of the Study: The study provides a greater understanding of genome similarity and dissimilarity between A. flavus and A. oryzae. The method can be an auxiliary technique for identifying A. flavus, A. oryzae.

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Section: Methodsmentioning

confidence: 99%

Genome‐wide nucleotide variation distinguishesAspergillus flavusfromAspergillus oryzaeand helps to reveal origins of atoxigenicA. flavusbiocontrol strains

Chang

2019

J Appl Microbiol

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“… c From reference 6 . d Chang et al ( 12 ), derived from isolate CA14. e Pseudochromosomes inferred from alignment to Aspergillus oryzae strain RIB40.…”

Section: Announcementmentioning

confidence: 99%

Chromosome-Level Aspergillus flavus Strain CA14 Genome Assembly

Weaver¹,

Mack²,

Gilbert³

2021

Microbiol Resour Announc

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“…In A. oryzae, culturing in the presence of the DOPA precursor tyrosine results in the production of DOPA melanin [52]. DOPA pathway inhibitors have prevented pigmentation in A. nidulans [51], A. flavus [48], and A. niger [48], although subsequent work seemed to cast doubt on these results [53]. It has been surmised that the black pigment of A. niger, known as aspergillin, is actually a polymerization of two different pigments, one brown and one green.…”

Section: Plos Onementioning

confidence: 99%

“…Consequently, the pigmentation of A. niger is acknowledged to be complex and is still under investigation [47]. Regardless, what is abundantly clear from the literature is that A. nidulans, A. flavus, and A. niger do not respond to DHN pathway inhibitors [48,51,53].…”

Section: Plos Onementioning

confidence: 99%

Identification of toxic mold species through Raman spectroscopy of fungal conidia

et al. 2020

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We use a 785 nm shifted excitation Raman difference (SERDS) technique to measure the Raman spectra of the conidia of 10 mold species of especial toxicological, medical, and industrial importance, including Stachybotrys chartarum, Penicillium chrysogenum, Aspergillus fumigatus, Aspergillus flavus, Aspergillus oryzae, Aspergillus niger, and others. We find that both the pure Raman and fluorescence signals support the hypothesis that for an excitation wavelength of 785 nm the Raman signal originates from the melanin pigments bound within the cell wall of the conidium. In addition, the major features of the pure Raman spectra group into profiles that we hypothesize may be due to differences in the complex melanin biosynthesis pathways. We then combine the Raman spectral data with neural network models to predict species classification with an accuracy above 99%. Finally, the Raman spectral data of all species investigated is made freely available for download and use.

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Identification of a copper-transporting ATPase involved in biosynthesis of A. flavus conidial pigment

Cited by 20 publications

References 41 publications

Genome‐wide nucleotide variation distinguishesAspergillus flavusfromAspergillus oryzaeand helps to reveal origins of atoxigenicA. flavusbiocontrol strains

Genome‐wide nucleotide variation distinguishesAspergillus flavusfromAspergillus oryzaeand helps to reveal origins of atoxigenicA. flavusbiocontrol strains

Chromosome-Level Aspergillus flavus Strain CA14 Genome Assembly

Identification of toxic mold species through Raman spectroscopy of fungal conidia

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