Identification of a Calmodulin-binding NAC Protein as a Transcriptional Repressor in Arabidopsis

Kim, Ho Soo; Park, Byung Ouk; Yoo, Jae Hyuk; Jung, Myun Sook; Lee, Sang Min; Han, Hay Ju; Kim, Kyung Eun; Kim, Sun Ho; Lim, Chae Oh; Yun, Dae‐Jin; Lee, Sang Yeol; Chung, Woo Sik

doi:10.1074/jbc.m705217200

Cited by 118 publications

(110 citation statements)

References 57 publications

(82 reference statements)

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“…These proteins have a conserved N-terminal NAC domain (originally found in no apical meristem, ATAFs [Arabidopsis transcription activation factor] and cup-shaped cotyledon), whereas the C-terminal domain is highly variable (Figure 2). The CaM binding NAC protein (CBNAC) is a Ca 2+ -dependent CaM binding transcriptional repressor, and its repressor activity is enhanced by binding to Ca 2+ /CaM (Kim et al, 2007;Yoon et al, 2008). Its DNA cis-element is a GCTT core sequence flanked on both sides by other frequently repeating sequences (TTGCTTANNNNNNAAG) (Kim et al, 2007).…”

Section: Impacts Of Cellular Ca 2+ Changes On Gene Expressionmentioning

confidence: 99%

“…The CaM binding NAC protein (CBNAC) is a Ca 2+ -dependent CaM binding transcriptional repressor, and its repressor activity is enhanced by binding to Ca 2+ /CaM (Kim et al, 2007;Yoon et al, 2008). Its DNA cis-element is a GCTT core sequence flanked on both sides by other frequently repeating sequences (TTGCTTANNNNNNAAG) (Kim et al, 2007).…”

Section: Impacts Of Cellular Ca 2+ Changes On Gene Expressionmentioning

confidence: 99%

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Coping with Stresses: Roles of Calcium- and Calcium/Calmodulin-Regulated Gene Expression

Ali²,

et al. 2011

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Abiotic and biotic stresses are major limiting factors of crop yields and cause billions of dollars of losses annually around the world. It is hoped that understanding at the molecular level how plants respond to adverse conditions and adapt to a changing environment will help in developing plants that can better cope with stresses. Acquisition of stress tolerance requires orchestration of a multitude of biochemical and physiological changes, and most of these depend on changes in gene expression. Research during the last two decades has established that different stresses cause signal-specific changes in cellular Ca 2+ level, which functions as a messenger in modulating diverse physiological processes that are important for stress adaptation. In recent years, many Ca 2+ and Ca 2+ /calmodulin (CaM) binding transcription factors (TFs) have been identified in plants. Functional analyses of some of these TFs indicate that they play key roles in stress signaling pathways. Here, we review recent progress in this area with emphasis on the roles of Ca 2+ -and Ca 2+ /CaMregulated transcription in stress responses. We will discuss emerging paradigms in the field, highlight the areas that need further investigation, and present some promising novel high-throughput tools to address Ca 2+ -regulated transcriptional networks.

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Section: Impacts Of Cellular Ca 2+ Changes On Gene Expressionmentioning

confidence: 99%

Section: Impacts Of Cellular Ca 2+ Changes On Gene Expressionmentioning

confidence: 99%

Coping with Stresses: Roles of Calcium- and Calcium/Calmodulin-Regulated Gene Expression

Ali²,

et al. 2011

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“…Although most NAC TFs are transcriptional activators (8,37) some, e.g. Arabidopsis CALMODULIN BINDING NAC and VND-INTER-ACTING2 (VNI2), function as repressors (9,45). VNI2 inhibited transcriptional activation by VND TFs involved in xylem vessel formation, possibly by forming inactive heterodimers with the VND proteins, and had intrinsic repression activity typical of an active repressor (9).…”

Section: Discussionmentioning

confidence: 99%

Senescence-associated Barley NAC (NAM, ATAF1,2, CUC) Transcription Factor Interacts with Radical-induced Cell Death 1 through a Disordered Regulatory Domain

Kjaersgaard

Jensen

Christiansen

et al. 2011

Journal of Biological Chemistry

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Background: Plant NAC transcription factors (TF) are important regulators of senescence. Results: Senescence-associated barley HvNAC013 uses intrinsic disorder in transcriptional activation and interactions. Conclusion: Radical induced cell death 1 exploits the intrinsic disorder of HvNAC013 and other TFs for interactions without structure induction in HvNAC013. Significance: This first structural characterization of NAC intrinsic disorder may reveal general features of important TF regulatory interactions.

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“…Yeast Split Ubiquitin Assay-The yeast split ubiquitin assay was performed as described previously (32,33). Saccharomyces cerevisiae strain JD53 was used for all experiments.…”

Section: Construction Of Deletion Mutants Of Atmkp1 and Site-directedmentioning

confidence: 99%

Regulation of MAPK Phosphatase 1 (AtMKP1) by Calmodulin in Arabidopsis

Lee

Song

Kim

et al. 2008

Journal of Biological Chemistry

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The mitogen-activated protein kinases (MAPKs) are key signal transduction molecules, which respond to various external stimuli. The MAPK phosphatases (MKPs) are known to be negative regulators of MAPKs in eukaryotes. We screened an Arabidopsis cDNA library using horseradish peroxidase-conjugated calmodulin (CaM), and isolated AtMKP1 as a CaM-binding protein. (MEKK). The MAPKs are activated by MEK via the phosphorylation of threonine and tyrosine residues within a conserved TXY motif (14, 15). MAPKs are inactivated by protein phosphatases such as tyrosine-specific phosphatase, serine/threonine-specific phosphatase, and dual specificity phosphatase, through specific dephosphorylation of threonine and tyrosine residues within the TXY consensus sequence (16, 17). MAPK phosphatases (MKPs) belong to the dual specificity phosphatase (DsPTP) family and dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues (18). Only five members of the MKPs, including the previously described AtDsPTP1, IBR5, PHS1, and AtMKP1, are predicted to exist in the Arabidopsis genome, and these five MKPs are responsible for the modulation of 20 potential MAPKs (18,19). The MKP/MAPK ratio in Arabidopsis is disproportionately low compared with other eukaryotes, implying that plants may possess integrated and complicated mechanisms for the regulation of MAPK signaling through MKPs.Plant MKPs play important roles in various biotic and abiotic stress responses. AtMKP1 was reported to be involved in genotoxic stress signaling (20) and AtMKP2 in the regulation of the cellular response to oxidative stress in Arabidopsis (21).

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Identification of a Calmodulin-binding NAC Protein as a Transcriptional Repressor in Arabidopsis

Cited by 118 publications

References 57 publications

Coping with Stresses: Roles of Calcium- and Calcium/Calmodulin-Regulated Gene Expression

Coping with Stresses: Roles of Calcium- and Calcium/Calmodulin-Regulated Gene Expression

Senescence-associated Barley NAC (NAM, ATAF1,2, CUC) Transcription Factor Interacts with Radical-induced Cell Death 1 through a Disordered Regulatory Domain

Regulation of MAPK Phosphatase 1 (AtMKP1) by Calmodulin in Arabidopsis

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