Identification of a Burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinant

DeShazer, David; Waag, David M.; Fritz, David L.; Woods, Donald E.

doi:10.1006/mpat.2000.0430

Cited by 163 publications

(160 citation statements)

References 47 publications

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“…Live attenuated tularemia and melioidosis vaccines delivered intradermally or by ip protected against challenge by these routes, but not against an aerosol challenge [33,34]. In addition, DD3008 delivered ip did not protect against a low-dose aerosol challenge with ATCC 23344 [21]. Finally, Atkins et al [13] demonstrated that a live attenuated B. pseudomallei ilvI transposon mutant protected mice against a lethal ip melioidosis challenge, but it did not protect against an ATCC 23344 aerosol challenge with 1.9 × 10 4 bacteria (19 times the LD 50 ).…”

Section: Discussionmentioning

confidence: 99%

“…B. mallei ILV1, ∆(ilvI) (BMAA0437-BMAA0497), is a branched-chain amino acid auxotroph derived from SR1 (see below). B. mallei DD3008, wcbB::pGSV3, is a capsule mutant that is avirulent in animals [18,21]. Bacteria were grown at 37 • C on LB agar (Lennox L agar, Sigma, St. Louis, MO) or in LB broth (Lennox L broth).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…The PCR products were directionally cloned into pGRV2 and the resulting plasmid was designated pDD120. This plasmid was mobilized into B. mallei SR1 via biparental mating with E. coli S17-1 (pDD120), as previously described [21]. Pm was used to counterselect E. coli and Gm was used to select for transconjugants containing pDD120 integrated into the chromosome via homologous recombination.…”

Section: Construction Of B Mallei Ilv1mentioning

confidence: 99%

“…We aerogenically vaccinated mice with two genetically defined B. mallei mutants, ILV1 and DD3008 [21]. ILV1-vaccinated animals received a predicted inhaled dose of 7.3 × 10 4 bacteria and DD3008-vaccinated animals received a predicted inhaled dose of 1.2 × 10 3 bacteria.…”

Section: B Mallei Ilv1 Is Attenuated In Mice and Generates A Th1-likmentioning

confidence: 99%

See 3 more Smart Citations

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Ulrich

Amemiya

Waag

et al. 2005

Vaccine

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Section: Construction Of B Mallei Ilv1mentioning

confidence: 99%

Section: B Mallei Ilv1 Is Attenuated In Mice and Generates A Th1-likmentioning

confidence: 99%

See 2 more Smart Citations

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Ulrich

Amemiya

Waag

et al. 2005

Vaccine

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“…OPSII appears to play a role in resistance to serum killing and virulence in the diabetic rat model of infection [22,23]. The polysaccharide capsule also appears to be an important virulence factor, and a transposon mutant that failed to produce exopolysaccharide was markedly attenuated in Syrian hamsters [24].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Atkins

Prior

Mack

et al. 2002

Journal of Medical Microbiology

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Comparative genome‐wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis

et al. 2016

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Burkholderia contaminans MS14 shows significant antimicrobial activities against plant and animal pathogenic fungi and bacteria. The antifungal agent occidiofungin produced by MS14 has great potential for development of biopesticides and pharmaceutical drugs. However, the use of Burkholderia species as biocontrol agent in agriculture is restricted due to the difficulties in distinguishing between plant growth‐promoting bacteria and the pathogenic bacteria. The complete MS14 genome was sequenced and analyzed to find what beneficial and virulence‐related genes it harbors. The phylogenetic relatedness of B. contaminans MS14 and other 17 Burkholderia species was also analyzed. To research MS14′s potential virulence, the gene regions related to the antibiotic production, antibiotic resistance, and virulence were compared between MS14 and other Burkholderia genomes. The genome of B. contaminans MS14 was sequenced and annotated. The genomic analyses reveal the presence of multiple gene sets for antimicrobial biosynthesis, which contribute to its antimicrobial activities. BLAST results indicate that the MS14 genome harbors a large number of unique regions. MS14 is closely related to another plant growth‐promoting Burkholderia strain B. lata 383 according to the average nucleotide identity data. Moreover, according to the phylogenetic analysis, plant growth‐promoting species isolated from soils and mammalian pathogenic species are clustered together, respectively. MS14 has multiple antimicrobial activity‐related genes identified from the genome, but it lacks key virulence‐related gene loci found in the pathogenic strains. Additionally, plant growth‐promoting Burkholderia species have one or more antimicrobial biosynthesis genes in their genomes as compared with nonplant growth‐promoting soil‐isolated Burkholderia species. On the other hand, pathogenic species harbor multiple virulence‐associated gene loci that are not present in nonpathogenic Burkholderia species. The MS14 genome as well as Burkholderia species genome show considerable diversity. Multiple antimicrobial agent biosynthesis genes were identified in the genome of plant growth‐promoting species of Burkholderia. In addition, by comparing to nonpathogenic Burkholderia species, pathogenic Burkholderia species have more characterized homologs of the gene loci known to contribute to pathogenicity and virulence to plant and animals.

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Identification of a Burkholderia mallei polysaccharide gene cluster by subtractive hybridization and demonstration that the encoded capsule is an essential virulence determinant

Cited by 163 publications

References 47 publications

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Aerogenic vaccination with a Burkholderia mallei auxotroph protects against aerosol-initiated glanders in mice

Characterisation of an acapsular mutant of Burkholderia pseudomallei identified by signature tagged mutagenesis

Comparative genome‐wide analysis reveals that Burkholderia contaminans MS14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis

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