Identification of a broad-spectrum recessive gene in Brassica rapa and molecular analysis of the eIF4E gene family to develop molecular markers

Kim, Jinhee; Kang, Won-Hee; Yang, Heng; Park, Suhyoung; Jang, Chang-Hwan; Yu, Hee‐Ju; Kang, Byoung-Cheorl

doi:10.1007/s11032-013-9878-0

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…TuRB03, a monodominant gene controlling pathotype 4 resistance, was localized to a 7.9 cM region on N6 in B. napus 24 . retr01 represents the first mapped recessive gene in Brassica species 25 ; other recessive genes from B. rapa include rnt1 and trs, which were mapped to R6 and A4, respectively 26,27 . Using bulked segregant analysis by sequencing (BSA-seq), Shopan et al 28 identified another recessive gene, retr03, in B. juncea.…”

Section: Turnip Mosaic Virusmentioning

confidence: 99%

“…This eIF4E-mediated mode of resistance is generally strong and broad spectrum 197,198 . In Brassica, the recessive R genes to TuMV, including retr01, retr02, and trs, have been identified to encode eIF (iso)4E 25,27,199 . Furthermore, researchers induced different key amino acid mutations in eIF(iso)4E by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and host eIF(iso)4E, and transgenic plants with eIF(iso)4E variants display high and broad-spectrum resistance 31 .…”

Section: Pyramiding Of Qualitative and Quantitative Loci To Acquire Dmentioning

confidence: 99%

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An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

Fang

Yang

et al. 2020

Hortic Res

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Brassica species include many economically important crops that provide nutrition and health-promoting substances to humans worldwide. However, as with all crops, their production is constantly threatened by emerging viral, bacterial, and fungal diseases, whose incidence has increased in recent years. Traditional methods of control are often costly, present limited effectiveness, and cause environmental damage; instead, the ideal approach is to mine and utilize the resistance genes of the Brassica crop hosts themselves. Fortunately, the development of genomics, molecular genetics, and biological techniques enables us to rapidly discover and apply resistance (R) genes. Herein, the R genes identified in Brassica crops are summarized, including their mapping and cloning, possible molecular mechanisms, and application in resistance breeding. Future perspectives concerning how to accurately discover additional R gene resources and efficiently utilize these genes in the genomic era are also discussed.

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Section: Turnip Mosaic Virusmentioning

confidence: 99%

Section: Pyramiding Of Qualitative and Quantitative Loci To Acquire Dmentioning

confidence: 99%

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

Fang

Yang

et al. 2020

Hortic Res

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“…Kim et al . () mapped a gene, trs , located on A4 that conferred resistance to TuMV isolates CHN2/CHN3/CHN4/CHN5 and which may be another recessive gene tightly linked to retr02 or another allele. In general, recessive genes can impart resistance to multiple TuMV isolates.…”

Section: Inheritance Mapping and Cloning Of Resistance Genesmentioning

confidence: 99%

TuMV management for brassica crops through host resistance: retrospect and prospects

Zhang

et al. 2019

Plant Pathology

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Turnip mosaic virus (TuMV), the only potyvirus known to infect brassicas, is a devastating virus threatening many economically important brassica crops, including cabbage, Chinese cabbage, oilseed rape and mustard. TuMV disease, which was first discovered in the United States, is now found worldwide, especially in Europe, Asia and North America. TuMV results in a yield loss of up to 70% and has a wide host range, infecting most cruciferous plants, as well as many non-cruciferous species. This virus is also characterized by high pathotype diversity because of its highly variable genome structure and has been divided into 12 pathotypes. These characteristics, as well as its nonpersistent transmission mode by as many as 89 aphid species, mean the disease is difficult to prevent through traditional methods such as the application of chemicals, prompting researchers to seek host resistance for effective control. During the last decade, extensive studies have been conducted to investigate inheritance, mapping and cloning of the TuMV resistance genes, and several NB-LRR-or eIF-encoding loci with divergent molecular mechanisms have been uncovered. These studies have greatly facilitated resistance breeding for brassica crops and have advanced our understanding of virusÀhost interactions.

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“…Host factors are essential in the viral infection cycle and therefore recessive resistance against viruses can be induced if one or more host factors are absent or mutated via a mechanism known as resistance by loss of susceptibility (Charron et al ., ; Bastet et al ., ). Natural recessive resistance genes involved in the plant–virus pathosystem have been successfully exploited in diverse crop species, including pepper ( Capsicum annuum ), lettuce ( Lactuca sativa ), pea ( Pisum sativum ), common bean ( Phaseolus vulgaris ), barley ( Hordeum vulgare ), tomato ( Solanum lycopersicum ), melon ( Cucumis melo ), Chinese cabbage ( Brassica rapa ), and rice ( Oryza sativa ) (Ruffel et al ., ; Nicaise et al ., ; Gao et al ., ; Kanyuka et al ., ; Albar et al ., ; Nieto et al ., ; Naderpour et al ., ; Kim et al ., ; Gauffier et al ., ), and the majority of these genes are associated with the eukaryotic translation initiation factor 4E (eIF4E) or its isoform, eIF(iso)4E.…”

Section: Introductionmentioning

confidence: 98%

mentioning

confidence: 99%

Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance

Gao

Luo

Ding

et al. 2019

Molecular Plant Pathology

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Soybean mosaic virus (SMV), a potyvirus, is the most prevalent and destructive viral pathogen in soybean‐planting regions of China. Moreover, other potyviruses, including bean common mosaic virus (BCMV) and watermelon mosaic virus (WMV), also threaten soybean farming. The eukaryotic translation initiation factor 4E (eIF4E) plays a critical role in controlling resistance/susceptibility to potyviruses in plants. In the present study, much higher SMV‐induced eIF4E1 expression levels were detected in a susceptible soybean cultivar when compared with a resistant cultivar, suggesting the involvement of eIF4E1 in the response to SMV by the susceptible cultivar. Yeast two‐hybrid and bimolecular fluorescence complementation assays showed that soybean eIF4E1 interacted with SMV VPg in the nucleus and with SMV NIa‐Pro/NIb in the cytoplasm, revealing the involvement of VPg, NIa‐Pro, and NIb in SMV infection and multiplication. Furthermore, transgenic soybeans silenced for eIF4E were produced using an RNA interference approach. Through monitoring for viral symptoms and viral titers, robust and broad‐spectrum resistance was confirmed against five SMV strains (SC3/7/15/18 and SMV‐R), BCMV, and WMV in the transgenic plants. Our findings represent fresh insights for investigating the mechanism underlying eIF4E‐mediated resistance in soybean and also suggest an effective alternative for breeding soybean with broad‐spectrum viral resistance.

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Identification of a broad-spectrum recessive gene in Brassica rapa and molecular analysis of the eIF4E gene family to develop molecular markers

Cited by 23 publications

References 37 publications

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

An update on the arsenal: mining resistance genes for disease management of Brassica crops in the genomic era

TuMV management for brassica crops through host resistance: retrospect and prospects

Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance

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