Identification, Ligninolytic Enzyme Activity and Decolorization Potential of Two Fungi Isolated from a Distillery Effluent Contaminated Site

Pant, Deepak; Adholeya, Alok

doi:10.1007/s11270-007-9366-4

Cited by 50 publications

(19 citation statements)

References 45 publications

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“…Both 18S rRNA gene and ITS region are extensively used to study the fungal community structure in various ecosystems (Roose-Amsaleg et al 2004;Lai et al 2007;Pant and Adholeya 2007b;Curlevski et al 2010). However, there are still controversial opinions on the sensitivity and accuracy of the two regions.…”

Section: Discussionmentioning

confidence: 99%

“…Both the internal transcribed spacer (ITS) region and 18S ribosomal RNA genes were applied to uncover the fungal diversity in DGGE fingerprinting (Lord et al 2002;Anderson et al 2003a, b;Pant and Adholeya 2007b;Weber et al 2009;Liu et al 2015). Fragments of 18S rDNA are considered as practical markers in molecular genotyping analyses (Vainio and Hantula 2000;Green et al 2004;Duong et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR–DGGE

Liu

Cai

et al. 2015

World J Microbiol Biotechnol

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Both the internal transcribed spacer (ITS) region and 18S rRNA genes are broadly applied in molecular fingerprinting studies of fungi. However, the differences in those two ribosomal RNA regions are still largely unknown. In the current study, three sets of most suitable subunit ribosomes in ITS and 18S rRNA were compared using denaturing gradient gel electrophoresis (DGGE) under the optimum experimental conditions. Ten samples from both aquatic and soil environments were tested. The results revealed that the ITS region produced range-weighted richness in the range 36-361, which was significantly higher than that produced by 18S rDNA. There was a similar tendency in terms of the Shannon-Weaver diversity index and community dynamics in both water and soil samples. Samples from water and soil were better separated using ITS than 18S rDNA in principal component analysis of DGGE bands. Our study suggests that the ITS region is more precise and has more potential than 18S rRNA genes in fungal community analysis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR–DGGE

Liu

Cai

et al. 2015

World J Microbiol Biotechnol

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“…Lac activity was determined using the 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulfonic acid as substrate by monitoring absorbance intensification at 420 nm (ε = 36000/Mcm). The reaction mixture contained 800 µL of supernatant sample and 200 µL of 1.0 mM 2,2 -azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) in 0.1 M sodium acetate pH 5.0 (Pant & Adholeya 2007).…”

Section: P Chrysosporium Immobilizationmentioning

confidence: 99%

“…Ligninolytic processes from P. chrysosporium have been applied to industrial effluent detoxification (Pant & Adholeya 2007), mostly coming from textile and petrochemical industries, bleaching and delignification processes in the paper and pulp industries (Wesenberg et al 2003;Gomathi et al 2012). The capacities of this fungus to remove xenobiotic substances and produce polymeric products make up a useful tool for bioremediation purposes.…”

Section: Introductionmentioning

confidence: 99%

Enzyme production by immobilized Phanerochaete chrysosporium using airlift reactor

et al. 2014

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Enzyme production by immobilized Phanerochaete chrysosporium was evaluated in airlift bioreactor and agitated cultures. Free mycelium and immobilized mycelium on alginate beads were tested in the decolourization of 50 and 500 mg/L of Remazol Brilliant Blue R. Dye concentration did not inhibit the fungi development in all tests. In addition, high decolourization percentage of dye was found with free mycelium (99%) in agitated flasks and with immobilized mycelium in airlift (98%). However, decolourization period by immobilized mycelium (120 h) was greater than that by the free mycelium (14 h). Important manganese peroxidase, lignine peroxidase and laccase activities were identified in decolourization process. Manganese peroxidase appeared to be promoted by high dye concentrations during the treatment with immobilized mycelium, but this enzyme was not detected with free mycelium in airlift. Bioreactor prompted also laccase and lignine peroxidase actions in both tests; free mycelium registered a maximum laccase action of 31.569 × 10 3 U/L in 70 h, whereas immobilized mycelium registered 1.680 × 10 3 U/L in 170 h, while lignine peroxidase secretion by free P. chrysosporium was higher (1.300 × 10 3 U/L) than immobilized mycelium (1.250 × 10 3 U/L). Maximum laccase activity coincided with the maximum percentage of decolourization, however, high peroxidase activity was identified from the start of dye treatment.

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“…DNA extraction, PCR Amplification and sequencing were used to identify the fungi as described by Pant and Adholeya (2007).…”

Section: Morphologicalmentioning

confidence: 99%

Potency of <i>Trichoderma aureoviride</i> UPM 09 and <i>Fusarium equiseti UPM 09 in the pretreatment and hydrolysis of lignocelluolosic biomass

Farouq¹,

Yerima²,

Shehu³

et al. 2017

Bayero J. Pure App. Sci.

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Two new strains of fungi, namely, Trichoderma aureoviride UPM 09 and Fusarium equiseti UPM 09 were isolated from elephant dung and identified morphologically and through the use of molecular assay. Their genomic DNA was extracted using Epicenter kit ® . PCR amplification of their genomic DNA was successfully conducted with BIOMETRA Tpersonal/Tprofessional Thermocycler (Germany) using ITS-1 forward primer (5' TCC GTA GGT GAA CCT GCG G3') and the ITS-4 reverse primer (5' GCT GCG TTC TTC TTG ATC GAT GC 3'). The sequences of the fungal strains were deposited in the NCBI (USA) Gen Bank Database and were assigned accession numbers (in parenthesis) and were identified as Trichoderma aureoviride strain UPM 09 (JN811063) and Fusarium equiseti strain UPM 09 (JN811061). The two fungal strains individually and in consortium were then used for the pretreatment of rice husk (RH), rubber wood saw dust (RW) and oil palm empty fruit bunch (EFB) using solid state cultivation (SSC) and submerged cultivation (SMC). The amount of glucose, reducing sugars and protein from the pretreated lignocellulose biomass was determined using glucose analyzer, DNS reagent and Biorad assay, respectively. The result of this study, therefore, shows that native fungi possess potentials for use in the pretreatment of lignocelluloses biomass.

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Identification, Ligninolytic Enzyme Activity and Decolorization Potential of Two Fungi Isolated from a Distillery Effluent Contaminated Site

Cited by 50 publications

References 45 publications

Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR–DGGE

Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR–DGGE

Enzyme production by immobilized Phanerochaete chrysosporium using airlift reactor

Potency of <i>Trichoderma aureoviride</i> UPM 09 and <i>Fusarium equiseti UPM 09 in the pretreatment and hydrolysis of lignocelluolosic biomass

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