Abstract:Two new strains of fungi, namely, Trichoderma aureoviride UPM 09 and Fusarium equiseti UPM 09 were isolated from elephant dung and identified morphologically and through the use of molecular assay. Their genomic DNA was extracted using Epicenter kit ® . PCR amplification of their genomic DNA was successfully conducted with BIOMETRA Tpersonal/Tprofessional Thermocycler (Germany) using ITS-1 forward primer (5' TCC GTA GGT GAA CCT GCG G3') and the ITS-4 reverse primer (5' GCT GCG TTC TTC TTG ATC GAT GC 3'). The s… Show more
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