Identification, distribution pattern of IS<i>231</i>elements in<i>Bacillus thuringiensis</i>and their phylogenetic analysis

Huang, Tianpei; Liu, Jingjing; Song, Fuping; Shu, Changlong; Qiu, Jian; Guan, Xiong; Huang, Dafang; Zhang, Jie

doi:10.1016/j.femsle.2004.09.037

Cited by 6 publications

(4 citation statements)

References 30 publications

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“…The potential adverse effects of chemical pesticides on human health and the environment have prompted researchers to find alternative pest control strategies based on natural products that are environmentally friendly and less harmful to humans and non‐target organisms (Huang et al, ). We reported here the efficacy of the application of commercial formulations of S. feltiae , Azadirachtin , B. thuringiensis and B. bassiana against T. absoluta .…”

Section: Discussionmentioning

confidence: 99%

“…Biopesticides based on entomopathoges and botanical products represent important pest control options for farmers to manage T. absoluta, especially in organic production, where economic, environmental or human health risks may entirely preclude the use of chemical pesticides (Abd El-Ghanny, Abdel-Razek, Djelouah, & Moussa, 2018;Amizadeh, Hejazi, Niknam, & Arzanlou, 2015). These natural products offer a number of advantages over chemical pesticides because they have a reduced risk of pest resistance, are environmentally friendly, and harmless to humans and non-target organisms (Huang et al, 2004).The entomopathogenic bacterium Bacillus thuringiensis (Bt) is widely used for controlling various agricultural pests. More than 3,000 species, belonging to 16 orders of insects including T. absoluta, have been reported as susceptible to B. thuringiensis (Abd El-Ghany, Abdel-Razek, Ebadah, & Mahmoud, 2016;Melo, Soccol, & Soccol, 2016 Van-Damme et al, 2016), and the entomopathogenic fungi Beauveria bassiana (Abd El-Ghanny et al, 2018;Rodriguez, Gerding, & France, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of some biorational insecticides against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory and greenhouse conditions in Kuwait

Jallow

Dahab

Albaho

et al. 2018

J Applied Entomology

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The tomato leafminer, Tuta absoluta, is one of the most destructive pests of tomato worldwide. Management of the pest is mainly based on chemical insecticides. Reliance on insecticides is difficult to sustain because of unintended long‐term adverse effects on the environment and human health. Consequently, there is a need to develop pest management strategies that ensure the production of high‐quality products, while at the same time ensuring environmental sustainability and maximum consumer protection. We evaluated the efficacy the biopesticides: Azadirachtin, Bacillus thuringiensis, Steinernema feltiae and Beauveria bassiana individually and in combination against T. absoluta under laboratory and greenhouse conditions. When second instar larvae were exposed to tomato leaf discs treated with Azadirachtin (3 g/L), B. thuringiensis (0.5 g/L) or B. bassiana (1.5 g/L), 70%–86%, 55%–65%, and 45.5%–58.5% mortality was observed, respectively. Steinernema feltiae (1,000 IJs/ml) was the least effective biopesticide, with 26%–42% mortality. In the greenhouse trials on tomato, pest infestation (mines/10 leaves/plant) and fruits damaged were significantly lower on plants treated with Azadirachtin + B. thuringiensis or Azadirachtin + B. bassiana compared to plants treated with Azadirachtin, B. thuringiensi, B. bassiana or S. feltiae alone. Azadirachtin + B. thuringiensis and Azadirachtin + B. bassiana resulted in 90% and 81% reduction in fruits damaged in the summer experiments, respectively, and 96% and 91% in winter. The most severe pest infestation was observed on plants treated with S. feltia. The results indicate that the biopesticides, except S. feltia, can contribute to T. absoluta control in greenhouse tomato crops. In particular, the combined use of Azadirachtin with B. thuringiensis or B. bassiana provided the highest level of control of the pest. The potential for including these biopesticides in an overall sustainable integrated pest management programme for T. absoluta is discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficacy of some biorational insecticides against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory and greenhouse conditions in Kuwait

Jallow

Dahab

Albaho

et al. 2018

J Applied Entomology

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“…pBMB0228 is mobilizable and can resolve into its two separate plasmids after conjugative transfer; those plasmids can later fuse back together. In addition to often being located on conjugative plasmids, the dispersion of cry genes is probably assisted by their association with various IS elements, including IS231, IS232, IS240, ISBt1, and ISBt2, and transposons, including Tn4430 and Tn5401 (159,160,170).…”

Section: Virulence Plasmids Of B Cereus and B Thuringiensismentioning

confidence: 99%

Virulence Plasmids of Spore-Forming Bacteria

Adams

Wisniewski

et al. 2014

Microbiol Spectr

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Plasmid-encoded virulence factors are important in the pathogenesis of diseases caused by spore-forming bacteria. Unlike many other bacteria, the most common virulence factors encoded by plasmids in Clostridium and Bacillus species are protein toxins. Clostridium perfringens causes several histotoxic and enterotoxin diseases in both humans and animals and produces a broad range of toxins, including many pore-forming toxins such as C. perfringens enterotoxin, epsilon-toxin, beta-toxin, and NetB. Genetic studies have led to the determination of the role of these toxins in disease pathogenesis. The genes for these toxins are generally carried on large conjugative plasmids that have common core replication, maintenance, and conjugation regions. There is considerable functional information available about the unique tcp conjugation locus carried by these plasmids, but less is known about plasmid maintenance. The latter is intriguing because many C. perfringens isolates stably maintain up to four different, but closely related, toxin plasmids. Toxin genes may also be plasmid-encoded in the neurotoxic clostridia. The tetanus toxin gene is located on a plasmid in Clostridium tetani, but the botulinum toxin genes may be chromosomal, plasmid-determined, or located on bacteriophages in Clostridium botulinum. In Bacillus anthracis it is well established that virulence is plasmid determined, with anthrax toxin genes located on pXO1 and capsule genes on a separate plasmid, pXO2. Orthologs of these plasmids are also found in other members of the Bacillus cereus group such as B. cereus and Bacillus thuringiensis. In B. thuringiensis these plasmids may carry genes encoding one or more insecticidal toxins.

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“…ISs played an important role in genome reshuffling and evolution by facilitating horizontal gene transfer and mediating homologous recombination between multiple copies present in a given genome (Mahillon et al, 1999). The diversity and distribution of some well-known ISs that are generally structurally associated with genes coding for parasporal crystal proteins in B. thuringiensis have been widely studied Leonard et al, 1997;Rosso & Delecluse, 1997;Joung & Cote, 2003;Huang et al, 2004). However, the entire ISs content of the B. thuringiensis genome has never been reported.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and diversity of insertion sequences in the genome of Bacillus thuringiensis YBT-1520 and comparison with other Bacillus cereus group members

Qiu

Wang

et al. 2010

FEMS Microbiology Letters

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Members of the Bacillus cereus group are closely related bacteria that exhibit highly divergent pathogenic properties. Sequencing of Bacillus thuringiensis ssp. kurstaki strain YBT-1520 revealed an increased number of insertion sequences (ISs) compared with those of the published B. cereus group genomes. Although some of these ISs have been observed and summarized in B. thuringiensis previously, a genomic characterization of their content is required to reveal their distribution and evolution. The result shows that the larger number of transposase coding genes on YBT-1520 chromosome is mainly caused by the amplification of IS231C, IS232A and ISBth166. Some functional genes have been disrupted through the insertion of ISs, preferentially IS231C. By comparing the Southern hybridization profiles of different B. thuringiensis strains, the existence of ISBth166 was mainly found in serovar kurstaki and the recent expansion of IS231C between different kurstaki isolates was suggested. In addition to revealing the ISs profile in YBT-1520 as well as the comparison in the B. cereus group, this study will contribute to further comparative analyses of multiple B. thuringiensis strains aimed at understanding the IS-mediated genomic rearrangements among them.

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Identification, distribution pattern of IS231elements inBacillus thuringiensisand their phylogenetic analysis

Cited by 6 publications

References 30 publications

Efficacy of some biorational insecticides against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory and greenhouse conditions in Kuwait

Efficacy of some biorational insecticides against Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) under laboratory and greenhouse conditions in Kuwait

Virulence Plasmids of Spore-Forming Bacteria

Prevalence and diversity of insertion sequences in the genome of Bacillus thuringiensis YBT-1520 and comparison with other Bacillus cereus group members

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