Identification dehydratase domains from Schizochytrium sp. and Shewanella sp. and distinct functions in biosynthesis of fatty acids

Man, Yanli; Zhang, Yuting; Jiang, Jia-Yi; Zhao, Quanyu; Ren, Lu‐Jing

doi:10.1007/s00449-021-02644-1

Cited by 5 publications

(7 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 8 domains are involved in ARA biosynthesis, including 3-ketoacyl synthase (KS), malonyl-CoA:ACP transacylase (MAT), ketoacyl reductase (KR), chain length factor (CLF), dehydratase (DH), enoyl reductase (ER), and phosphopantetheinyl transferase (PPTase) . Similar to the desaturase/elongase pathway, acetyl-CoA is first carboxylated to malonyl-CoA, which is then attached to an active acyl carrier protein (ACP) by AT to produce acetyl-ACP . Finally, acetyl-ACP enters the cycle of dehydration, condensation, and extension, respectively, catalyzed by DH, KS, and ER.…”

Section: Biosynthetic Pathway Of Ara In Fungimentioning

confidence: 99%

“…24 Similar to the desaturase/elongase pathway, acetyl-CoA is first carboxylated to malonyl-CoA, which is then attached to an active acyl carrier protein (ACP) by AT to produce acetyl-ACP. 25 Finally, acetyl-ACP enters the cycle of dehydration, condensation, and extension, respectively, catalyzed by DH, KS, and ER. In contrast to the desaturase/elongase pathway, the PKS pathway carries many advantages, including a lower NADPH requirement and simpler fatty acid profile.…”

Section: Biosynthetic Pathway Of Ara In Fungimentioning

confidence: 99%

See 1 more Smart Citation

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Guo

Peng

et al. 2022

ACS Synth. Biol.

View full text Add to dashboard Cite

Arachidonic acid is an essential ω-6 polyunsaturated fatty acid, which plays a significant role in cardiovascular health and neurological development, leading to its wide use in the food and pharmaceutical industries. Traditionally, ARA is obtained from deep-sea fish oil. However, this source is limited by season and is depleting the already threatened global fish stocks. With the rapid development of synthetic biology in recent years, oleaginous fungi have gradually attracted increasing attention as promising microbial sources for large-scale ARA production. Numerous advanced technologies including metabolic engineering, dynamic regulation of fermentation conditions, and multiomics analysis were successfully adapted to increase ARA synthesis. This review summarizes recent advances in the bioengineering of oleaginous fungi for ARA production. Finally, perspectives for future engineering approaches are proposed to further improve the titer yield and productivity of ARA.

show abstract

Section: Biosynthetic Pathway Of Ara In Fungimentioning

confidence: 99%

Section: Biosynthetic Pathway Of Ara In Fungimentioning

confidence: 99%

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Guo

Peng

et al. 2022

ACS Synth. Biol.

View full text Add to dashboard Cite

show abstract

“…The DH domains in eukaryotic PUFA synthase may have the same characteristics, which still needs to be confirmed. Man et al showed that heterologous expression of two DH FabA domains can increase the proportion of unsaturated fatty acids, while heterologous expression of DH PKS helps increase saturated fatty acids ( Man et al, 2021 ) in E. coli . Overexpression of DH PKS in A. limacinum OUC168 showed increased contents of both C20:4 and total fatty acids ( Liu et al, 2018 ).…”

Section: Function Of Domains In Eukaryotic Pufa Synthasementioning

confidence: 99%

“…Overexpression of DH PKS in A. limacinum OUC168 showed increased contents of both C20:4 and total fatty acids ( Liu et al, 2018 ). Heterologous expression of DH PKS and DH FabA domains in E. coli increased the biomass and weakened the inhibitory effect of mid-chain fatty acids on cell growth ( Man et al, 2021 ). In addition, Xie et al ( Xie et al, 2020 ) pointed out that there are some differences in terms of the functions of these two DH domains, DH FabA -1 and DH FabA -2.…”

Section: Function Of Domains In Eukaryotic Pufa Synthasementioning

confidence: 99%

“…For example, homology-modeling servers such as SWISS-MODEL ( Waterhouse et al, 2018 ) and Phyre2 ( Kelley et al, 2015 ) can predict the structure of proteins by relying on structures of known proteins with high similarity, and more recently, AlphaFold2 ( Jumper et al, 2021 ) has been used with a machine learning approach for the de novo structure prediction of proteins with atomic accuracy. Several reports mentioned the structures of eukaryotic PUFA synthase domains predicted by homology modeling methods, such as the three DH domains ( Man et al, 2021 ) and the CLF domain ( Li et al, 2018 ). The development of bioinformatics and computational biology studies has provided quick and convenient tools for revealing the catalytic activity and enzymatic modification of PUFA synthases.…”

Section: Challenges and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms

Guo

Dong

Wang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Polyunsaturated fatty acids (PUFAs) are important nutrients that play important roles in human health. In eukaryotes, PUFAs can be de novo synthesized through two independent biosynthetic pathways: the desaturase/elongase pathway and the PUFA synthase pathway. Among them, PUFAs synthesized through the PUFA synthase pathway typically have few byproducts and require fewer reduction equivalents. In the past 2 decades, numerous studies have been carried out to identify, analyze and engineer PUFA synthases from eukaryotes. These studies showed both similarities and differences between the eukaryotic PUFA synthase pathways and those well studied in prokaryotes. For example, eukaryotic PUFA synthases contain the same domain types as those in prokaryotic PUFA synthases, but the number and arrangement of several domains are different; the basic functions of same-type domains are similar, but the properties and catalytic activities of these domains are somewhat different. To further utilize the PUFA synthase pathway in microbial cell factories and improve the productivity of PUFAs, many challenges still need to be addressed, such as incompletely elucidated PUFA synthesis mechanisms and the difficult genetic manipulation of eukaryotic hosts. In this review, we provide an updated introduction to the eukaryotic PUFA synthase pathway, summarize the functions of domains and propose the possible mechanisms of the PUFA synthesis process, and then provide future research directions to further elucidate and engineer the eukaryotic PUFA synthase pathway for the maximal benefits of humans.

show abstract

Nucleic acid-based scaffold systems and application in enzyme cascade catalysis

Peng-chen

Ren

2022

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Identification dehydratase domains from Schizochytrium sp. and Shewanella sp. and distinct functions in biosynthesis of fatty acids

Cited by 5 publications

References 33 publications

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms

Nucleic acid-based scaffold systems and application in enzyme cascade catalysis

Contact Info

Product

Resources

About