Identification, cloning and characterization of R2R3-MYB gene family in canola (<i>Brassica napus</i>L.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death

Chen, Bisi; Niu, Fangfang; Liu, Wu-Zhen; Yang, Bo; Zhang, Jingxiao; Ma, Ji; Cheng, Hao; Han, Feng; Jiang, Yuan‐Qing

doi:10.1093/dnares/dsv040

Cited by 53 publications

(29 citation statements)

References 45 publications

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“…CATs acted downstream of both MYB78 and MYB15, which regulate reactive oxygen species (ROS)-dependent cell death in AZ separation825. Two CAT genes were confirmed to express in abscissions in both ethylene and water-deficit stress treatments (Fig.…”

Section: Discussionmentioning

confidence: 90%

Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

Liao

Yang

et al. 2016

Sci Rep

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Cassava plants (Manihot esculenta Crantz) resist environmental stresses by shedding leaves in leaf pulvinus abscission zones (AZs), thus leading to adaptation to new environmental conditions. Little is known about the roles of cassava R2R3 MYB factors in regulating AZ separation. Herein, 166 cassava R2R3 MYB genes were identified. Evolutionary analysis indicated that the 166 R2R3 MYB genes could be divided into 11 subfamilies. Transcriptome analysis indicated that 26 R2R3 MYB genes were expressed in AZs across six time points during both ethylene- and water-deficit stress-induced leaf abscission. Comparative expression profile analysis of similar SOTA (Self Organizing Tree Algorithm) clusters demonstrated that 10 R2R3 MYB genes had similar expression patterns at six time points in response to both treatments. GO (Gene Ontology) annotation confirmed that all 10 R2R3 MYB genes participated in the responses to stress and ethylene and auxin stimuli. Analysis of the putative 10 R2R3 MYB promoter regions showed that those genes primarily contained ethylene- and stress-related cis-elements. The expression profiles of the genes acting downstream of the selected MYBs were confirmed to be involved in cassava abscission zone separation. All these results indicated that R2R3 MYB plays an important regulatory role in AZ separation.

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Section: Discussionmentioning

confidence: 90%

Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

Liao

Yang

et al. 2016

Sci Rep

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“…The term ‘hypersensitive‐like cell death’ or similar is often used simply when cell death is observed without any explicit link to R ‐genes, the defence response or disease resistance (e.g. Chen et al , ; Király et al , ; Kumar and Kirti, ; Na et al , ; Wei et al , ).…”

Section: Conceptsmentioning

confidence: 99%

“…The term 'hypersensitive-like cell death' or similar is often used simply when cell death is observed without any explicit link to R-genes, the defence response or disease resistance (e.g. Chen et al, 2016;Király et al, 2008;Kumar and Kirti, 2015;Na et al, 2015;Wei et al, 2016). This review will take a rather narrow view of HR, confined to the localized rapid cell death and excluding the other associated responses induced by the activation of NLR proteins.…”

Section: How Should Hr Be Defined?mentioning

confidence: 99%

The plant hypersensitive response: concepts, control and consequences

Balint‐Kurti

2019

Molecular Plant Pathology

399

252

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Summary The hypersensitive defence response is found in all higher plants and is characterized by a rapid cell death at the point of pathogen ingress. It is usually associated with pathogen resistance, though, in specific situations, it may have other consequences such as pathogen susceptibility, growth retardation and, over evolutionary timescales, speciation. Due to the potentially severe costs of inappropriate activation, plants employ multiple mechanisms to suppress inappropriate activation of HR and to constrain it after activation. The ubiquity of this response among higher plants despite its costs suggests that it is an extremely effective component of the plant immune system.

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“…Zhou et al [ 8 ] identified 247 BnbZIPs in B. napus and performed detailed structural analyses. Chen et al [ 33 ] identified 76 R2R3 MYBs in the B. napus genome through excavating expressed sequence tags (ESTs), and the transcript abundance level analysis during abiotic conditions and ABA treatment identified a group of MYBs that responded to one or more treatments. Recently, Hajiebrahimi et al [ 34 ] identified 249 R2R3 MYBs in B. napus , and the authors reported that these genes were highly expressed in the roots under high salinity treatment after 24 h. Three MYB genes, BnaMYB21, BnaMYB141, and BnaMYB148 have been suggested improve salt-tolerance in B. napus .…”

Section: Introductionmentioning

confidence: 99%

Exploring transcription factors reveals crucial members and regulatory networks involved in different abiotic stresses in Brassica napus L.

et al. 2018

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BackgroundBrassica napus (B. napus) encompasses diverse transcription factors (TFs), but thorough identification and characterization of TF families, as well as their transcriptional responsiveness to multifarious stresses are still not clear.ResultsTotally 2167 TFs belonging to five families were genome-widely identified in B. napus, including 518 BnAP2/EREBPs, 252 BnbZIPs, 721 BnMYBs, 398 BnNACs and 278 BnWRKYs, which contained some novel members in comparison with existing results. Sub-genome distributions of BnAP2/EREBPs and BnMYBs indicated that the two families might have suffered from duplication and divergence during evolution. Synteny analysis revealed strong co-linearity between B. napus and its two ancestors, although chromosomal rearrangements have occurred and 85 TFs were lost. About 7.6% and 9.4% TFs of the five families in B. napus were novel genes and conserved genes, which both showed preference on the C sub-genome. RNA-Seq revealed that more than 80% TFs were abiotic stress inducible and 315 crucial differentially expressed genes (DEGs) were screened out. Network analysis revealed that the 315 DEGs are highly co-expressed. The homologous gene network in A. thaliana revealed that a considerable amount of TFs could trigger the differential expression of targeted genes, resulting in a complex clustered network with clusters of genes responsible for targeted stress responsiveness.ConclusionsWe identified and characterized five TF families in B. napus. Some crucial members and regulatory networks involved in different abiotic stresses have been explored. The investigations deepen our understanding of TFs for stress tolerance in B. napus.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1417-z) contains supplementary material, which is available to authorized users.

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Identification, cloning and characterization of R2R3-MYB gene family in canola (Brassica napusL.) identify a novel member modulating ROS accumulation and hypersensitive-like cell death

Cited by 53 publications

References 45 publications

Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

The plant hypersensitive response: concepts, control and consequences

Exploring transcription factors reveals crucial members and regulatory networks involved in different abiotic stresses in Brassica napus L.

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