Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

Liao, Wenbin; Yang, Yiling; Li, Yayun; Wang, Gan; Peng, Ming

doi:10.1038/srep32006

Cited by 34 publications

(46 citation statements)

References 44 publications

(111 reference statements)

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“…Since their identification, they have been found to be extensively dispersed in plants and communicate with additional transcription factors [36]. MYB transcription factors are involved in the regulation of plant growth and development in various species like in soybean, they are involved in regulation of flower color [37] and regulation of signal transduction pathways in Arabidopsis, rice and cassava [38]. Biosynthesis of secondary metabolites is regulated in Arabidopsis and Medicago [36].…”

Section: Regulatory Roles Of Transcription Factors In Plantsmentioning

confidence: 99%

Transcription Factors and MicroRNA Interplay: A New Strategy for Crop Improvement

Jangra¹,

Chaudhary²,

Yadav³

2018

Transcriptional and Post-Transcriptional Regulation

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MicroRNAs (miRNAs) and transcription factors are master regulators of the cellular system. Plant genomes contain thousands of protein-coding and non-coding RNA genes; which are differentially expressed in different tissues at different times during growth and development. Complex regulatory networks that are controlled by transcription factors and microRNAs, which coordinate gene expression. Transcription factors, the key regulators of plant growth and development, are the targets of the miRNAs families. The combinatorial regulation of transcription factors and miRNAs guides the appropriate implementation of biological events and developmental processes. The resources on the regulatory cascades of transcription factors and miRNAs are available in the context of human diseases, but these resources are meager in case of plant diseases. On the other hand, it is also important to understand the cellular and physiological events needed to operate the miRNAs networks. The relationship between transcription factors and miRNA in different plant species described in this chapter will be of great interest to plant scientists, providing better insights into the mechanism of action and interactions among transcription factors (TFs) and miRNA networks culminating in improving key agronomic traits for crop improvement to meet the future global food demands.

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Section: Regulatory Roles Of Transcription Factors In Plantsmentioning

confidence: 99%

Transcription Factors and MicroRNA Interplay: A New Strategy for Crop Improvement

Jangra¹,

Chaudhary²,

Yadav³

2018

Transcriptional and Post-Transcriptional Regulation

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“…Transcription factors play an important role in regulation the physiological mechanism of plant drought tolerance. Several members of the MYB family in cassava have been reported to be involved in drought stress and chilling signal transduction pathways (Liao et al 2016b;Li et al 2017). The MeMYB2 is a typical member of the MYB family and includes two MYB family DNA binding domains in its protein amino acid sequence.…”

Section: Discussionmentioning

confidence: 99%

“…The MeMYB2 is a typical member of the MYB family and includes two MYB family DNA binding domains in its protein amino acid sequence. The MeMYB2 gene is classi ed into the R2R3-MYB subclass (Liao et al 2016b). The Arabidopsis homologous gene AtMYB2 of MeMYB2, together with AtMYC2, is involved in the regulation of the drought stress response gene rd22 in the ABA-dependent signaling pathway (Abe et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies, we con rmed that the cassava leaf abscission zones were extremely easy to shed under drought conditions and the cassava abscission zones can be used as a modle tissue to evaluate and screen the drought tolerance genes (Liao et al 2016a;Liao et al 2016b). Therefore, in order to understand the response of MeMYB2 to drought in cassava, we used cassava leaf abscission zones to evaluate the MeMYB2 response under drought.…”

Section: Memyb2 Responds To Drought Stress In Cassavamentioning

confidence: 99%

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Natural variations of MeMYB2 in cassava varieties covering a wide range of the reactive oxygen scavenging enzymes under drought

Wang

Guo

et al. 2020

Preprint

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Background Cassava ( Manihot esculenta Crantz) is an important tropical crop with excellent stress tolerance and stress resistance genes. The antioxidant enzymes play an important role in regulating the cassava plants respond to stress. To date, little is known the relationship between natural variations of MYB genes that have been proven to regulate stress tolerance in model plants and the diversity of reactive oxygen scavenging enzymes under drought in cassava varieties. Therefore, we conducted this study to discover natural variations in MYB2 gene in association with the reactive oxygen scavenging enzymes. Results Here, the relationship between the natural variation of MYB2 gene and the reactive oxygen scavenging enzymes under drought in cassava were analyzed. Natural variation of antioxidant enzymes in leaf and roots of 97 cassava varieties under drought analyses indicated that the variation of the various antioxidant enzymes is very different among the cassava varieties. MeMYB2 expression profiling analysis indicated that MeMYB2 was up-regulated obviously when induced by drought stress. Re-sequencing analysis of the 97 cassava varieties indicated that the MeMYB2 gene region included 87 single nucleotide variants (SNPs) and two insertion/deletion variants (Insert/ Deletion, Indel), the SNP locus of the coding region of MeMYB2 gene can be divided into 29 haplotypes, including 7 major haplotypes. These 7 major haplotypes can be classified into two categories, one of which is more closely related to wild cassava genotype W14, which indicates that MeMYB2 is positively selected during cassava breeding. Correlation analysis between MeMYB2 and drought tolerance showed that 12 functional SNPs in the MeMYB2 coding region were significantly associated with CAT activity, proline content, SOD activity and other traits, and the prediction of interaction with key genes of drought tolerance was further confirmed that MeMYB2 and key genes involved in CAT, proline and SOD can interact through intermediate metabolic pathways. Conclusions Some natural variations exist in the MeMYB2 , which might be involved in regulating the reactive oxygen scavenging enzymes (SOD and CAT) changes under stress. This study will facilitate the understanding of the roles of MYB s in regulating the reactive oxygen scavenging enzymes.

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“…During drought stress, reactive oxygen species (ROS) and proline play the important roles in the regulation of cassava drought stress, effective ROS clearance mechanism can delay leaf senescence and slow the formation abscission zones in leaf petioles to control leaf abscission (Liao et al 2016a). Active oxygen scavenging enzymes such as proline, catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX) play the important roles in cassava drought tolerance (Liao et al 2016a;Liao et al 2016b;Xu et al 2013a). Xu et al overexpressed two ROS scavenging enzyme MeCu/ZnSOD and MeCAT1 in cassava, which greatly enhanced the ability of cassava to scavenge reactive oxygen species, resisting drought stress, leaf greening, senescence in transgenic cassava when compared with the wild-type controls (Xu et al 2013a, Xu et al 2013b).…”

Section: Introductionmentioning

confidence: 99%

Natural variations of MeMYB2 in cassava varieties covering a wide range of the reactive oxygen scavenging enzymes under drought

Wang¹,

Guo²,

Li³

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

Background Cassava (Manihot esculenta Crantz) is an important tropical crop with excellent stress tolerance and stress resistance genes. The antioxidant enzymes play an important role in regulating the cassava plants respond to stress. To date, little is known the relationship between natural variations of MYB genes that have been proven to regulate stress tolerance in model plants and the diversity of reactive oxygen scavenging enzymes under drought in cassava varieties. Therefore, we conducted this study to discover natural variations in MYB2 gene in association with the reactive oxygen scavenging enzymes.Results Here, the relationship between the natural variation of MYB2 gene and the reactive oxygen scavenging enzymes under drought in cassava were analyzed. Natural variation of antioxidant enzymes in leaf and roots of 97 cassava varieties under drought analyses indicated that the variation of the various antioxidant enzymes is very different among the cassava varieties. MeMYB2 expression profiling analysis indicated that MeMYB2 was up-regulated obviously when induced by drought stress. Re-sequencing analysis of the 97 cassava varieties indicated that the MeMYB2 gene region included 87 single nucleotide variants (SNPs) and two insertion/deletion variants (Insert/ Deletion, Indel), the SNP locus of the coding region of MeMYB2 gene can be divided into 29 haplotypes, including 7 major haplotypes. These 7 major haplotypes can be classified into two categories, one of which is more closely related to wild cassava genotype W14, which indicates that MeMYB2 is positively selected during cassava breeding. Correlation analysis between MeMYB2 and drought tolerance showed that 12 functional SNPs in the MeMYB2 coding region were significantly associated with CAT activity, proline content, SOD activity and other traits, and the prediction of interaction with key genes of drought tolerance was further confirmed that MeMYB2 and key genes involved in CAT, proline and SOD can interact through intermediate metabolic pathways.Conclusions Some natural variations exist in the MeMYB2, which might be involved in regulating the reactive oxygen scavenging enzymes (SOD and CAT) changes under stress. This study will facilitate the understanding of the roles of MYBs in regulating the reactive oxygen scavenging enzymes.

show abstract

Genome-wide identification of cassava R2R3 MYB family genes related to abscission zone separation after environmental-stress-induced abscission

Cited by 34 publications

References 44 publications

Transcription Factors and MicroRNA Interplay: A New Strategy for Crop Improvement

Transcription Factors and MicroRNA Interplay: A New Strategy for Crop Improvement

Natural variations of MeMYB2 in cassava varieties covering a wide range of the reactive oxygen scavenging enzymes under drought

Natural variations of MeMYB2 in cassava varieties covering a wide range of the reactive oxygen scavenging enzymes under drought

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