Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

Cao, Heping; Zhang, Lin; Tan, Xiaofeng; Long, Hongxu; Shockey, Jay

doi:10.1371/journal.pone.0088409

Cited by 24 publications

(23 citation statements)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FAD2 and FADx were highly expressed in mid-late stages of developing seeds; whereas FAD3 was expressed higher in early stages of seed, also in agreement with published results [14]. All five OLE s were highly expressed in mid-late stage of developing tung seeds, similar results to what we reported previously [26]. Our expression analysis provided novel insights into the potential role of PDAT s in tung oil biosynthesis by showing that PDAT1-1 , 1-4 , and 2-2 were expressed more in mid-late stages of developing seeds but the other three PDAT genes were expressed more in the early stages of developing seeds, which were not reported previously.…”

Section: Discussionsupporting

confidence: 92%

“…OLEs are the major proteins in plant oil bodies. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that the five tung OLE genes represented the five OLE subfamilies and all contained the “proline knot” motif (PX5SPX3P) shared among 65 OLE from 19 tree species [26]. We confirmed the five tung tree OLE genes coding for small hydrophobic proteins.…”

Section: Resultssupporting

confidence: 63%

See 1 more Smart Citation

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

Zhang

Liu

Long

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Resultssupporting

confidence: 63%

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

Zhang

Liu

Long

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Inhibition of the major oleosin gene (18 kD) expression in Arabidopsis resulted in unusually larger oil bodies, disruption of storage organelles and significant decrease in the amount of lipids (Siloto et al, 2006). On the contrary, overexpression of soybean oleosin gene (24 kD) in transgenic rice seed significantly improved the lipid content with massive smaller oil bodies (Cao et al, 2014). In our work, oleosins were identified from both Vernicia and Jatropha, each with 6 distinctive members.…”

Section: Discussionmentioning

confidence: 54%

Differential Gene Regulation of Lipid Synthesis in the Developing Seeds of Two Biodiesel Tree Species, Jatropha and Vernicia

Yang¹,

Huai-yun²,

Peng³

et al. 2016

IJAB

View full text Add to dashboard Cite

The fatty acid compositions of Jatropha oil and Vernicia oil are strikingly different, which leads to a great difference in combustion performance, low temperature performance and oxidation stability. A comparative transcriptomic study was made in Vernicia and Jatropha, with a focus on the gene regulation of differential oil accumulation process. Transcriptome sequencing was conducted with seeds at the initial-and fast-stage of oil accumulation from both. More than 24 billion bases of cDNA sequence were obtained, with 49,583 and 45,414 high-quality unigenes identified for Vernicia and Jatropha seeds, respectively. Multiple comparative transcriptome approaches revealed a number of species-specific fatty acid desaturases (FAD2, FADX, FAH12 etc.) contributing to their differentiated fatty acid compositions in seeds of Vernicia and Jatropha. Meanwhile, the results suggested that DGAT majorly regulates TAG synthesis than PDAT in Vernicia seed, and PDAT may have more important role regulating TAG synthesis in Jatropha seed than in Vernicia seed. It was also implied that specific oleosins involving in oil bodies may have member bias and may affect lipid contents in seeds of Vernicia and Jatropha, as some of which were 30-50 fold up-regulated (with their RPKM values over 10,000 at fast-stage). Some important factors were identified and can differentially regulate lipid pathways in seeds of Vernicia and Jatropha.

show abstract

“…They provide fatty acids for membrane biogenesis and lead to obesity if excessive amounts of fats accumulate in adipose tissues (Chapman et Walther, 2009;Somerville et al, 2000). Plant TAGs are both for human consumption and for industrial applications (Cahoon et al, 2007;Dyer et al, 2008) including biofuel production (Carvalho et al, 2008;Chen et al, 2010b;Tai and Stephanopoulos, 2013;Winichayakul et al, 2013). Many trees contain up to 50%-80% of oil in the seeds and fruits (Cao et al, 2013;Chen et al, 2008;Zeng et al, 2014;Zhang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

Cao

2015

OMICS: A Journal of Integrative Biology

Self Cite

View full text Add to dashboard Cite

Trees contribute to enormous plant oil reserves because many trees contain 50%-80% of oil (triacylglycerols, TAGs) in the fruits and kernels. TAGs accumulate in subcellular structures called oil bodies/droplets, in which TAGs are covered by low-molecular-mass hydrophobic proteins called oleosins (OLEs). The OLEs/TAGs ratio determines the size and shape of intracellular oil bodies. There is a lack of comprehensive sequence analysis and structural information of OLEs among diverse trees. The objectives of this study were to identify OLEs from 22 tree species (e.g., tung tree, tea-oil tree, castor bean), perform genome-wide analysis of OLEs, classify OLEs, identify conserved sequence motifs and amino acid residues, and predict secondary and threedimensional structures in tree OLEs and OLE subfamilies. Data mining identified 65 OLEs with perfect conservation of the ''proline knot'' motif (PX5SPX3P) from 19 trees. These OLEs contained >40% hydrophobic amino acid residues. They displayed similar properties and amino acid composition. Genome-wide phylogenetic analysis and multiple sequence alignment demonstrated that these proteins could be classified into five OLE subfamilies. There were distinct patterns of sequence conservation among the OLE subfamilies and within individual tree species. Computational modeling indicated that OLEs were composed of at least three ahelixes connected with short coils without any b-strand and that they exhibited distinct 3D structures and ligand binding sites. These analyses provide fundamental information in the similarity and specificity of diverse OLE isoforms within the same subfamily and among the different species, which should facilitate studying the structurefunction relationship and identify critical amino acid residues in OLEs for metabolic engineering of tree TAGs.

show abstract

Identification, Classification and Differential Expression of Oleosin Genes in Tung Tree (Vernicia fordii)

Cited by 24 publications

References 79 publications

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

The Tung Tree (Vernicia Fordii) Genome Provides A Resource for Understanding Genome Evolution and Oil Improvement

Differential Gene Regulation of Lipid Synthesis in the Developing Seeds of Two Biodiesel Tree Species, Jatropha and Vernicia

Genome-Wide Analysis of Oleosin Gene Family in 22 Tree Species: An Accelerator for Metabolic Engineering of BioFuel Crops and Agrigenomics Industrial Applications?

Contact Info

Product

Resources

About