Identification, Characterization, Immunolocalization, and Biological Activity of Lucilin Peptide

Téllez, Germán Alberto; Zapata, Jesica Alejandra; Toro, Lily Johanna; Henao, Diana Carolina Toro; Bedoya, Juan Pablo; Rivera, Juan David; Rivas-Santiago, Bruno; Hoyos, Richard; Castaño, Jhon Carlos

doi:10.1016/j.actatropica.2018.06.003

Cited by 15 publications

(16 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its pathological manifestations are diverse, including diarrhea, abdominal pain, and tenesmus etc. In recent research, a cecropin-like peptide from maggot had antimicrobial activity against Gram-negative bacteria and immunomodulatory activities [ 26 ]. We speculated that one peptide responsible for antibacterial activity [ 34 ] had anti-inflammatory effects on IBD.…”

Section: Discussionmentioning

confidence: 99%

“…Consistently, the anti-inflammatory cytokine IL-10 is found to be down-regulated in UC models [ 24 , 25 ]. Recent research found that a cecropin-like peptide from maggot had antimicrobial activity against Gram-negative bacteria and immunomodulatory activities, which decreased the TNF-α production in human peripheral blood mononuclear cells (PBMCs) and induced cellular migration in human keratinocytes [ 26 ]. Our present study aimed to evaluate whether the addition of the maggot protein could protect mice with DSS-induced acute colitis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Wang

Luo

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a common chronic remitting disease but without satisfactory treatment. Maggots are known as a traditional Chinese medicine named as ‘wu gu chong’. The aim of the present study was to investigate the therapeutic effect of the maggot protein on dextran sulphate sodium (DSS)-induced colitis in C57BL/6 mice. In the present study, female C57BL/6 mice were given sterile water containing 3% DSS to establish the model of UC. Mice were randomly divided into five groups: control group (sterile water), model group (DSS), treatment group (DSS + maggot protein), mesalazine group (DSS + mesalazine), and maggot protein group (sterile water + maggot protein). The mental state, defecate traits, and changes in body weights were recorded daily. The disease activity index (DAI) as a disease severity criterion was calculated based on body weights and stool consistency and bleeding. All the mice were killed on the 12th day. Colon length, colon histological changes, and other inflammatory factors were analyzed and evaluated. The results showed that colitis models of mice were established successfully. Administration of maggot protein markedly suppressed the severity of UC compared with the DSS model group. Furthermore, maggot protein potently ameliorated DSS-induced weight loss, colon shortening, and colon histological injury. Moreover, the maggot protein exerted anti-inflammatory effects via inhibition of the activation of the nuclear factor κB (NFκB) signaling pathway. In summary, treatment by maggot protein was able to improve not only the symptoms of colitis, but also the microscopic inflammation in mice with DSS-induced colitis. The present study may have implications for developing an effective therapeutic strategy for inflammatory bowel diseases (IBDs).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Wang

Luo

et al. 2018

Bioscience Reports

View full text Add to dashboard Cite

show abstract

“…2 The mean hydrophobicity (H) is the total hydrophobicity (sum of all residue hydrophobicity indices) divided by the number of residues. 3 The relative hydrophobic moment of a peptide is its hydrophobic moment relative to that of a perfectly amphiphilic peptide. 4 Lowercase letters indicate the d-enantiomer of the proline.…”

Section: Peptide Design and Characteristicsmentioning

confidence: 99%

“…As an important part of innate immunity, antimicrobial peptides (AMPs) form the first line of defense against antimicrobial infections, as found in many microorganisms including insects, plants, and animals [2]. Furthermore, AMPs are known for their anti-inflammatory properties, anti-biofilm formation, promotion of tissue and injury repair, and anti-cancer effects [2][3][4]. Compared to traditional antibiotics, AMPs exert an antibacterial effect through non-receptor-mediated membrane permeability.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Cell Selectivity and Bactericidal Mechanism of Symmetric Peptides Centered on d-Pro–Gly Pairs

Jia

Wang

Zhang

et al. 2020

IJMS

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) have a unique action mechanism that can help to solve global problems in antibiotic resistance. However, their low therapeutic index and poor stability seriously hamper their development as therapeutic agents. In order to overcome these problems, we designed peptides based on the sequence template XXRXXRRzzRRXXRXX-NH 2 , where X represents a hydrophobic amino acid like Phe (F), Ile (I), and Leu (L), while zz represents Gly-Gly (GG) or d-Pro-Gly (pG). Showing effective antimicrobial activity against Gram-negative bacteria and low toxicity, designed peptides had a tendency to form an α-helical structure in membrane-mimetic environments. Among them, peptide LR pG (X: L, zz: pG) showed the highest geometric mean average treatment index (GM TI = 73.1), better salt, temperature and pH stability, and an additive effect with conventional antibiotics. Peptide LR pG played the role of anti-Gram-negative bacteria through destroying the cell membrane. In addition, peptide LR pG also exhibited an anti-inflammatory activity by effectively neutralizing endotoxin. Briefly, peptide LR pG has the potential to serve as a therapeutic agent to reduce antibiotic resistance owing to its high therapeutic index and great stability.

show abstract

Antioxidant enzyme regulating and intracellular ROS scavenging capacities of two novel bioactive peptides from white grub larvae (Polyphylla adstpersa) hydrolysate in A549 cells

2020

View full text Add to dashboard Cite

Identification, Characterization, Immunolocalization, and Biological Activity of Lucilin Peptide

Cited by 15 publications

References 54 publications

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

High Cell Selectivity and Bactericidal Mechanism of Symmetric Peptides Centered on d-Pro–Gly Pairs

Antioxidant enzyme regulating and intracellular ROS scavenging capacities of two novel bioactive peptides from white grub larvae (Polyphylla adstpersa) hydrolysate in A549 cells

Contact Info

Product

Resources

About