Identification, characterization, and expression analysis of calmodulin and calmodulin-like genes in grapevine (Vitis vinifera) reveal likely roles in stress responses

Vandelle, Elodie; Vannozzi, Alessandro; Wong, Darren Chern Jan; Danzi, Davide; Digby, Anne-Marie; Santo, Silvia Dal; Astegno, Alessandra

doi:10.1016/j.plaphy.2018.06.003

Cited by 58 publications

(45 citation statements)

References 93 publications

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“…We compared our results with the computational analysis of CaMs and CMLs of V. vinifera published by Vandelle et al [25]. In addition to the three VviCaMs and 62 VviCMLs described previously for V. vinifera, our analysis of the V. vinifera CaMs and CMLs sequences available at the CRIBI and NCBI databases identified seven additional VviCMLs, including VviCML48, VviCML105, VviCML106, VviCML107, VviCML108, VviCML109, and VviCML110 (Table 1; Figure 1).…”

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 72%

“…The grapevine genome was not an exception. Recently, based on the analysis of the available grapevine genome sequencing data, Vandelle et al [25] determined the presence of three CaM and 62 CML genes in the genome of the commonly cultivated grapevine V. vinifera. Using the publicly available gene expression datasets of different organs and tissues of V. vinifera cv.…”

Section: Discussionmentioning

confidence: 99%

“…V. vinifera is a close species to V. amurensis, and its genome was sequenced [26,27]. To design the specific primers, we retrieved the predicted CaMs and CMLs of V. vinifera PN20024 genotype (V2 proteome prediction) present in the Grapevine Genome Centro Di Ricerca Interdipartimentale Per Le Biotecnologie Innovative (CRIBI) Biotech Centre database using the CaM and CML protein sequences of A. thaliana downloaded from the Arabidopsis Information Resource (TAIR) database as queries as described [25]. In addition, we downloaded all CaM and CML protein sequences of V. vinifera predicted by an automated computational analysis and deposited to the National Centre for Biotechnology Information (NCBI) GenBank.…”

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

“…In addition to the three VviCaMs and 62 VviCMLs described previously for V. vinifera, our analysis of the V. vinifera CaMs and CMLs sequences available at the CRIBI and NCBI databases identified seven additional VviCMLs, including VviCML48, VviCML105, VviCML106, VviCML107, VviCML108, VviCML109, and VviCML110 (Table 1; Figure 1). The seven newly identified VviCMLs were named according to the procedure described by Vandelle et al [25] following the instructions of the international Super-Nomenclature Committee for Grape Gene Annotation [28]. The deduced amino acid sequences of the newly identified VviCMLs did not contain functional domains other than EF-hands (Table 1).…”

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

“…A phylogenetic analysis was performed on the full-length amino acid sequences of the CaMs and CMLs from V. vinifera, V. amurensis, and A. thaliana (Figure 1). The proteins were categorized into nine subgroups based on sequence similarities and relationships of CaMs and CMLs of the analyzed plant species and taking into attention the described classification for V. vinifera proteins [25]. The deduced amino acid sequences of the newly identified VviCMLs clustered into the subgroup 2 (VviCML107, VviCML108), subgroup 4 (VviCML105, VviCML109), subgroup 6 (VviCML106, VviCML110), and subgroup 7 (VviCML48).…”

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

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The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

Dubrovina

Aleynova

Ogneva

et al. 2019

Plants

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Plant calmodulins (CaMs) and calmodulin-like proteins (CMLs) are important plant Ca2+-binding proteins that sense and decode changes in the intracellular Ca2+ concentration arising in response to environmental stimuli. Protein Ca2+ sensors are presented by complex gene families in plants and perform diverse biological functions. In this study, we cloned, sequenced, and characterized three CaM and 54 CML mRNA transcripts of Vitis amurensis Rupr., a wild-growing grapevine with a remarkable stress tolerance. Using real-time quantitative RT-PCR, we analyzed transcript abundance of the identified VaCaMs and VaCMLs in response to water deficit, high salinity, high mannitol, cold and heat stresses. Expression of VaCaMs and 32 VaCMLs actively responded to the abiotic stresses and exhibited both positive and negative regulation patterns. Other VaCML members showed slight transcriptional regulation, remained essentially unresponsive or responded only after one time interval of the treatments. The substantial alterations in the VaCaM and VaCML transcript levels revealed their involvement in the adaptation of wild-growing grapevine to environmental stresses.

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Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

Section: Isolation Molecular Cloning and Sequencing Of Vacam And Vamentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

Dubrovina

Aleynova

Ogneva

et al. 2019

Plants

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Ultralong‐lasting plasma‐activated water inhibits pathogens and improves plant disease resistance in soft rot‐infected hydroponic lettuce

Ran,

Zhou,

Dong

et al. 2024

Plasma Processes & Polymers

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To address the short lifetime of plasma‐activated water (PAW), this work reports on the application of ultralong‐lasting PAW for plant disease treatment. Compared with lettuce without ultralong‐lasting PAW treatment, the treated lettuce solution showed c. 1 log10 colony forming units mL−1 decrease in pathogens, as well as improvements in leaf area, leaf weight, root length, and root weight by 75%, 20%, 108.33%, and 150%, respectively. The leaf withering/root mortality rate of PAW‐treated plants was significantly lower than those of non‐PAW‐treated plants. Besides, disease defense‐related enzymes, such as catalase and β−1,3‐glucanase, were respectively enhanced by 112.5% and 5.13% in PAW‐treated lettuce, while the plant defense‐related genes were upregulated to respond to the pathogen's invasion.

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Genome-wide Identification and Expression Analysis of CaM/CML Gene Family in Sacred Lotus (Nelumbo nucifera)

Gao

Damaris

et al. 2022

Plant Mol Biol Rep

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Identification, characterization, and expression analysis of calmodulin and calmodulin-like genes in grapevine (Vitis vinifera) reveal likely roles in stress responses

Cited by 58 publications

References 93 publications

The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

Ultralong‐lasting plasma‐activated water inhibits pathogens and improves plant disease resistance in soft rot‐infected hydroponic lettuce

Genome-wide Identification and Expression Analysis of CaM/CML Gene Family in Sacred Lotus (Nelumbo nucifera)

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