The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

Dubrovina, Alexandra S.; Aleynova, Olga A.; Ogneva, Zlata V.; Suprun, Andrey R.; Ananev, Alexey A.; Kiselev, Konstantin V.

doi:10.3390/plants8120602

Cited by 30 publications

(29 citation statements)

References 43 publications

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“…CML protein belongs to calcium-binding EF-hand family proteins, which play an important role in cellular calcium signalling cascades through the regulation of numerous target proteins (Ranty et al 2006;Shi and Du 2020). This protein has a role in salinity tolerance and genes related to CML are shown to be upregulated under salt stress (Zeng et al 2015;Dubrovina et al 2019;Shi and Du 2020) and has improved the salt tolerance of Arabidopsis by affecting abscisic acid mediated pathways (Magnan et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Asif

Garcia

Tilbrook

et al. 2021

Functional Plant Biol.

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Bread wheat (Triticum aestivum L.) is one of the most important food crops, however it is only moderately tolerant to salinity stress. To improve wheat yield under saline conditions, breeding for improved salinity tolerance of wheat is needed. We have identified nine quantitative trail loci (QTL) for different salt tolerance sub-traits in a recombinant inbred line (RIL) population, derived from the bi-parental cross of Excalibur × Kukri. This population was screened for salinity tolerance subtraits using a combination of both destructive and non-destructive phenotyping. Genotyping by sequencing (GBS) was used to construct a high-density genetic linkage map, consisting of 3236 markers, and utilised for mapping QTL. Of the nine mapped QTL, six were detected under salt stress, including QTL for maintenance of shoot growth under salinity (QG(1-5).asl-5A, QG(1-5).asl-7B) sodium accumulation (QNa.asl-2A), chloride accumulation (QCl.asl-2A, QCl.asl-3A) and potassium:sodium ratio (QK:Na.asl-2DS2). Potential candidate genes within these QTL intervals were shortlisted using bioinformatics tools. These findings are expected to facilitate the breeding of new salt tolerant wheat cultivars.

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Section: Discussionmentioning

confidence: 99%

Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Asif

Garcia

Tilbrook

et al. 2021

Functional Plant Biol.

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“…CMLs are a unique class of EF-hand proteins and specifically existed in plants. The CML gene family has been characterized in some plant species, such as Arabidopsis [ 9 ], rice [ 10 ], grapevine ( Vitis amurensis ) [ 11 ], Chinese cabbage (Brassica rapa) [ 12 ] and tomato ( Solanum lycopersicum ) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Calmodulin-Like (CML) Gene Family in Medicago truncatula: Genome-Wide Identification, Characterization and Expression Analysis

Sun

Guo

2020

IJMS

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Calcium is an important second messenger in mediating adaptation responses of plants to abiotic and biotic stresses. Calmodulin-like (CML) protein is an important calcium-signaling protein that can sense and decode Ca2+ signal in plants. Medicago truncatula is a model legume plant; however, investigations of MtCML proteins are limited. Using genome analysis and BLAST database searches, fifty MtCML proteins that possess EF-hand motifs were identified. Phylogenetic analysis showed that CML homologs between M. truncatula, Arabidopsis thaliana and Oryza sativa shared close relationships. Gene structure analysis revealed that these MtCML genes contained one to four conserved EF-hand motifs. All MtCMLs are localized to eight chromosomes and underwent gene duplication. In addition, MtCML genes were differentially expressed in different tissues of M. truncatula. Cis-acting elements in promoter region and expression analysis revealed the potential response of MtCML protein to abiotic stress and hormones. The results provide a basis of further functional research on the MtCML gene family and facilitate their potential use for applications in the genetic improvement on M. truncatula in drought, cold and salt stress environments.

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“…There were 50 and 32 CML genes in Arabidopsis and rice, respectively [ 22 , 42 ]. CML genes were recently also identified in tomato, Medicago, grapevine, and apple [ 33 , 43 , 44 , 45 , 46 , 47 ]. MpCML40 is the first CML gene identified in Pongamia.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to Arabidopsis and rice, several studies showed that CMLs in other plants were also involved in abiotic stress signaling. Expression of 32 CML genes in wild-growing grapevine ( Vitis amurensis ) was shown to be responsive to abiotic stresses, including drought, salt, heat, and cold [ 33 ]. In Glycine soja , GsCML27 participated in salt and osmotic stresses [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene MpCML40 from Semi-Mangrove Millettia pinnata

Zhang

Huang

Hou

et al. 2021

IJMS

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Salt stress is a major increasing threat to global agriculture. Pongamia (Millettia pinnata), a semi-mangrove, is a good model to study the molecular mechanism of plant adaptation to the saline environment. Calcium signaling pathways play critical roles in the model plants such as Arabidopsis in responding to salt stress, but little is known about their function in Pongamia. Here, we have isolated and characterized a salt-responsive MpCML40, a calmodulin-like (CML) gene from Pongamia. MpCML40 protein has 140 amino acids and is homologous with Arabidopsis AtCML40. MpCML40 contains four EF-hand motifs and a bipartite NLS (Nuclear Localization Signal) and localizes both at the plasma membrane and in the nucleus. MpCML40 was highly induced after salt treatment, especially in Pongamia roots. Heterologous expression of MpCML40 in yeast cells improved their salt tolerance. The 35S::MpCML40 transgenic Arabidopsis highly enhanced seed germination rate and root length under salt and osmotic stresses. The transgenic plants had a higher level of proline and a lower level of MDA (malondialdehyde) under normal and stress conditions, which suggested that heterologous expression of MpCML40 contributed to proline accumulation to improve salt tolerance and protect plants from the ROS (reactive oxygen species) destructive effects. Furthermore, we did not observe any measurable discrepancies in the development and growth between the transgenic plants and wild-type plants under normal growth conditions. Our results suggest that MpCML40 is an important positive regulator in response to salt stress and of potential application in producing salt-tolerant crops.

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The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis

Cited by 30 publications

References 43 publications

Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Identification of salt tolerance QTL in a wheat RIL mapping population using destructive and non-destructive phenotyping

Calmodulin-Like (CML) Gene Family in Medicago truncatula: Genome-Wide Identification, Characterization and Expression Analysis

Isolation and Functional Characterization of a Salt-Responsive Calmodulin-Like Gene MpCML40 from Semi-Mangrove Millettia pinnata

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