Identification by Flagellum Display of an Epithelial Cell- and Fibronectin-Binding Function in the SlpA Surface Protein of Lactobacillus brevis

Effect of Environmental Stresses on S-Layer Production in Lactobacillus acidophilus ATCC 4356

“…It has been proposed that S-layer plays a role as a protective sheath in Lactobacillus acidophilus ATCC 4356. In addition, some studies identified that the S-layer proteins of Lactobacilli were important for hydrophobicity, autoaggregation and adherence of this bacteria to different host surfaces (Frece et al, 2005;Greene & Klaenhammer, 1994;Hynonen et al, 2002;Kos et al, 2003;Pelletier et al, 1997;Sillanpaa et al, 2000;Toba et al, 1995;Vadillo-Rodriguez et al, 2004Van der Mei et al, 2003).…”

“…Several reports have appeared in which functions of S-layer are described or assumed (Boot et al, 1993;Jakava-Viljanen et al, 2002;Sara & Sleytr, 2000). However, no general function has been identified for S-layer proteins, but several lactobacillar S-layers have been identified as putative adhesions with affinity for intestinal epithelial cells, extracellular matrices and/ or to lipoteichoic acid (LTA) of other bacterial species (Avall-Jaaskelainen & Palva, 2005;Buck et al, 2005;Frece et al, 2005;Garrote et al, 2004;Hynonen et al, 2002;Kos et al, 2003;Velez et al, 2007;Vidgren et al, 1992). Of the various roles proposed for the bacterial S-layer, it is a protective sheath against hostile environment (Avall-Jaaskelainen & Palva, 2005;Frece et al, 2005;Khaleghi et al, 2010Khaleghi et al, , 2011Kos et al, 2003;Schar-Zammaretti et al, 2005).…”

Development of New Products: Probiotics and Probiotic Foods

“…The S-layer proteins act as adhesins in many bacteria such as lactobacilli and some representatives of the genus Bacillus, so they determine their adhesion to epithelial cells or extracellular matrix proteins [44,45,46,47].…”

Adhesive Properties of Lactobacillus brevis FSB-1 In Vivo

“…Furthermore, L. brevis PEL1 has been found to have a high binding affinity to human colonic mucin (Ouwehand et al, 2001). Additionally Hynonen et al (2002) reported that the S-layer protein of L. brevis ATCC 8287 affected the binding affinity of human Caco-2 and Intestine 407 cells, the endothelial cell line, EA-hy 926, and the urinary bladder cell line, T 24, and that it also immobilized fibronectin. In addition the S-layer protein of L. brevis is known to contribute to adhesion properties in gut epithelial cells (Kahala et al, 1997).…”