Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey

Kiliç, Ayşe; Kalender, Hakan; Eroksuz, Hatice; Muz, Adile; Tasdemir, Bülent

doi:10.1007/s11250-013-0394-3

Cited by 17 publications

(9 citation statements)

References 32 publications

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“…Ackermann and Brogden (2) state that the brochus, bronchulus, alveoli and glands perform many tasks, such as antimicrobial activity, inflammatory response and detoxification of toxins. In previous studies, Mannheimia, Pasteurella and Mycoplasma agents were most commonly detected by immunohistochemical examinations in bronchial, bronchiolar, alveolar, epithelial and inflammatory cells (3,8,16,24,27,30). In this study, the expression of COX-2, HMGB-1 and CD68 was found in the above-mentioned structures by immunohistochemical analysis.…”

Section: B High Magnification Of Immunopositive Macrophages In Peribsupporting

confidence: 58%

Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia

Yavuz¹,

Dinçel²

2020

Medycyna Weterynaryjna

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This study aimed to determine the expression of some cytokines, such as cyclooxygenase-2 (COX-2), High Mobility Group Box-1 (HMGB-1) and CD68, in the lung tissue of sheep with fibrinous bronchopneumonia by immunohistochemical methods. Forty sheep which had suffered from respiratory problems were brought for necropsy to the Faculty of Veterinary Medicine, Department of Pathology, Aksaray University, between November 2014 and December 2017. After necropsy, lung tissues grossly diagnosed with fibrinous bronchopneumonia were processed histologically, and stained histopathologically and immunohistochemically. Bacteriological culture was also applied to the lung tissues to isolate the agents. In histopathological examinations, congestion, red consolidation and grey consolidation stages were detected in the lung tissues. In such cases, we observed fibrin masses accumulated in some alveolar lumens, as well as inflammatory cell infiltrations of various extent in alveolar and bronchiolar lumens. In the interalveolar septum, a thickening was observed due to a fibrin mass and thrombotic vessels. Immunohistochemically, it was determined that COX-2 and HMGB-1 cytokines showed positive reactions, especially in bronchial, bronchiolar and alveolar epithelia, as well as goblet cells and macrophages. CD68 was found to be expressed in alveolar macrophages. COX-2 and HMGB-1, which have been implicated in the inflammatory response, were also shown to be expressed in fibrinous bronchopneumonia in sheep for the first time. Thus, these cytokines are thought to play a role in the pathogenesis of the disease. Moreover, their increased expression suggests that it may be helpful in the diagnosis of the disease.

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Section: B High Magnification Of Immunopositive Macrophages In Peribsupporting

confidence: 58%

Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia

Yavuz¹,

Dinçel²

2020

Medycyna Weterynaryjna

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“…The rate of detection of Mycoplasma directly from the lungs as per PCR results (87.8%) was higher than the detection rate via culturing technique, suggesting that the detection and diagnosis of Mycoplasma species may be carried out directly from the tissues, especially lungs, without the need for culturing techniques, which take at least 14 days, thus reducing the time needed for the diagnosis of Mycoplasma infections 16,20,22 . The current study used sulphadimidinetrimethoprim plus nystatin for the first time instead of thallous acetate as an inhibitor for the growth of gram-negative bacteria and fungi, and it provided excellent results for isolation without contamination.…”

Section: Discussionmentioning

confidence: 97%

“…DNA extraction was performed from both, lung tissue and Mycoplasma colonies (19)(20)(21)(22). Mycoplasma genomic DNA was extracted from lung tissue and Mycoplasma colonies using the gSYNC™ Geneaid kit (Korea) following the manufacturer's instructions.…”

Section: Dna Extractionmentioning

confidence: 99%

Diagnosis of Mycoplasma from Starlings Lungs

Hamad¹,

Al-AAlim²,

Al-Chalaby³

2019

J Pure Appl Microbiol

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Starlings have tremendous economic and environmental impacts because they can spread pathogensto livestock and poultry. These birds act as mechanical and biological carriers for different types of pathogens from and to their original habitat. The goal of this study was to ascertain the presence of Mycoplasma gallisepticum (MG) in the starlings' lungs and confirm their diagnosis using the PCR technique. We altered the supplements that were added to Mycoplasma culture media by using calf serum instead of horse serum and sulphadimidine plus trimethoprim with nystatin instead of thallous acetate. Eighty-five starlings were bought from hunters in the spring of 2019, and their lungs were harvested and divided into two portions, one for Mycoplasma cultivation and the other for DNA extraction. Fifty-nine (69.4%) samples were positive for Mycoplasma colonies, thereby yielding accurate results using alternative supplements in the culture media. PCR revealed the presence of Mycoplasma in 78.8% lung samples, while MG was detected in only 43.3% of the positive samples, indicating the presence of other species of Mycoplasma too. The current study is the first of its kind not only in Iraq but also in the world, investigating the presence of MG in the lungs of starling birds. This study revealed that MG is significantly prevalent in starlings and also suggests that other Mycoplasma species may be present in starlings.

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“…Süt örneklerinin bakteriyolojik ve Ma spesifik PCR ile incelenmesi neticesinde %81.7'sinde M. agalactiae saptamışlardır [13] . Özdemir ve Türkaslan [14] ise yaptıkları çalışmada, Bulaşıcı Agalaksi'den şüpheli 22 [10,[20][21][22] . Türkiye'de Bulaşıcı Agalaksi hastalığına yöne-lik spesifik çalışma sayısı çok az olmakla birlikte mevcut çalışmalar ile karşılaştırıldığında çalışmamızdaki etken izolasyon oranının düşük olduğu görülmektedir.…”

Section: Tartişma Ve Sonuçunclassified

“…Türkiye'de mikoplazmaların enfekte hayvanlardan izolasyonu ile ilgili birçok çalışma mevcut olmasına rağmen [8][9][10][11][12] , Bulaşıcı Agalaksi Hastalığı üzerindeki çalışma sayısı sınır-lıdır [13][14][15] . Çetinkaya ve ark.…”

Section: Introductionunclassified

Investigation of Contagious Agalactia by Bacteriological and PCR Methods in Sheep and Goats

Göçmen¹,

Ülgen²,

Çarlı³

et al. 2015

Kafkas Univ Vet Fak Derg

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Investigation of Contagious Agalactia by Bacteriological and PCR Methods in Sheep and Goats AbstractThe aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balıkesir, Çanakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples,147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.

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Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey

Cited by 17 publications

References 32 publications

Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia

Expression of COX-2, HMGB-1 and CD68 in lung tissue in sheep fibrinous bronchopneumonia

Diagnosis of Mycoplasma from Starlings Lungs

Investigation of Contagious Agalactia by Bacteriological and PCR Methods in Sheep and Goats

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