Identification by Combined Gas–Liquid Chromatography – Mass Spectrometry of Sterols Isolated from Scenedesmus obliquus Grown in Light and Heavy Water

Abstract

The sterol fraction from Scet~edesmus obliquus, grown in heavy water, has been shown by combined g.1.c. -mass spectrometry to contain A7~Z2-ergostadiene-3P-ol, A7-ergostenol, chondrillasterol, and A7-chondrillastenol. The same algae, grown in light water, contain these four sterols and, i n addition, ergosterol and corbisterol.La fraction des sttrols obtenus de Scenedesnl~ts obliqurts, une Algue croissant dans I'eau lourde, a t t t analyste par la combinaison c.p.v. et spectromttrie de masse. I1 est montrte que les sttrols isolts sont le A7.22-ergostadiene-3p-ol, le A'-ergostenol, le chondrillasterol et le A7-chondrillastenol. La msme algue croissant dans I'eau ordinaire contient aussi I'ergosttrol et le corbisttrol en plus des quatres autres sttrols d t j i mentionnts.

Absence of sterols constrains food quality of cyanobacteria for an invasive freshwater bivalve

“…Mass spectra were recorded between 50 and 600 amu in the El ionization mode. Sterols were identified by comparison with mass spectra of reference substances purchased from Sigma or Steraloids and/or mass spectra found in a self-generated spectra library or in the literature (e.g., Belanger et al 1973;Goad and Akihisa 1997;Toyama et al 19S2). The C-24 stereochemistry and the cis-trans isomery of sterols could not be identified with certainty, and thus, if procurable, was adopted from the literature.…”