Identification, biological evaluation, and improved biotransformation of a phenazine antioxidant using Streptomyces lomondensis S015 whole cells

Deng, Ru-Xiang; Yue, Sheng‐Jie; Wang, Wei; Hu, Hongbo; Zhang, Xuehong

doi:10.1016/j.procbio.2022.12.017

Cited by 4 publications

(3 citation statements)

References 47 publications

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“…Phenazine-1-carboxylic acid dioxygenase is another enzyme derived from Streptomyces, which plays a role in organic pollutants [146] . Finally, Streptomyces is known to produce transglutaminase, an enzyme used in food processing to improve the texture and quality of meat products [147] .…”

Section: Streptomyces Psammoticusmentioning

confidence: 99%

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

Beroigui,

Errachidi

2023

Prog Micobes Mol Biol

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This article emphasizes the various contributions of actinobacteria to the well-being of human life on our planet. Agrarian virtues begin with the substitution of chemical fertilizers with bio-fertilizing microorganisms which have a versatile potential for the benefit of soil and plants by playing complementary and interconnected roles. Thus, these microorganisms improve soil health through bioremediation phenomena and the elimination of several non-beneficial microorganisms. In agriculture, these microorganisms can be added to compost as inoculants to speed up the composting process and provide an additional source of beneficial microorganisms for the compost-treated soil. Streptomyces and other actinobacteria can also be used as biotechnological sources of herbicides and insecticides. In the medical and therapeutic sectors, this paper emphasizes the potential of actinobacteria, in particular Streptomyces species, in the production of antibiotics, antioxidant, and anticancer agents, opening up avenues for the creation of molecules with high benefits. In biotechnology, these totipotent microorganisms produce enzymes widely used in several industries, generating considerable revenue. Sporadic data accumulated on these types of microorganisms opens up many new avenues for exploiting these natural biocatalytic resources. Papers published in the last decade have exploded the amount of information that can be put to practical use. To maximize the value of these microorganisms, it would be advisable to create common threads between the themes that bring together the areas of expertise where these microorganisms could potentially be exploited. This bibliographical synthesis is a contribution to the development of a targeted database.

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Section: Streptomyces Psammoticusmentioning

confidence: 99%

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

Beroigui,

Errachidi

2023

Prog Micobes Mol Biol

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“…A stock culture of a newly isolated fungus strain (Aspergillus sclerotiorum) was maintained on a yeast peptone dextrose (YPD) medium. The YPD medium contained (g L −1 ): glucose 30.0, peptone 10.0, and yeast extract 5.0 [29]. Phenazine-1-carboxylic acid (purity ≥ 98%) was purchased from Nonglebio Co., Ltd., Shanghai, China.…”

Section: Selection Of Chemicals and Mediamentioning

confidence: 99%

“…Currently, various genera of microorganisms capable of biotransformation of PCA have been reported, such as Nocardia, Mycobacterium, Sphingomonas, and Streptomyces [18][19][20][21]29]. In addition to bacteria, some studies have demonstrated PCA modification by growing cells of filamentous fungi [22,23].…”

Section: Whole-cell Biocatalysis Of Phenazine 1-carboxylic Acid By As...mentioning

confidence: 99%

Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid

et al. 2023

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In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products, such as phenazine compounds. The precursor metabolite of most phenazine derivatives in Pseudomonas spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain Aspergillus sclerotiorum was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was designed by screening optimal reaction conditions and carbon sources. Hence, Aspergillus sclerotiorum exhibited desirable adaptation by the consumption of different carbon sources and maximum whole-cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including a PCA concentration of 1120 mg/L, a pH of 7.0, a temperature of 25 °C, a rotation rate of 200 rpm, and dry cell weight of 15 g/L for 60 h; thus, 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of the repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle, but remained stable in all five cycles with the provision of a glucose supplement. These findings present the prospect of using filamentous fungi for the whole-cell biocatalysis of phenazine in enormous amounts and the efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic-based mechanism of the strain responsible for PCA biotransformation.

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Constructing a novel “turn-on” fluorescent sensor based on phenazine macrocycle through halogen bond interaction for the naked-eye detection of phenylenediamine isomers

Che

Tao

et al. 2023

Sensors and Actuators B: Chemical

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Identification, biological evaluation, and improved biotransformation of a phenazine antioxidant using Streptomyces lomondensis S015 whole cells

Cited by 4 publications

References 47 publications

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid

Constructing a novel “turn-on” fluorescent sensor based on phenazine macrocycle through halogen bond interaction for the naked-eye detection of phenylenediamine isomers

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