Identification, association, and expression analysis of ZmNAC134 gene response to phosphorus deficiency tolerance traits in maize at seedling stage

Sahito, Javed Hussain; Zheng, Fangliang; Tang, Haitao; He, Xuan; Luo, Bowen; Zhou, Xiao; Ma, Ping; Ding, Xin; Liú, Dan; Wu, Long‐Fei

doi:10.1007/s10681-020-02634-6

Cited by 5 publications

(6 citation statements)

References 60 publications

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“…LD decay indicated that ZmNAC9 was less affected by natural variation and it was not under the selection pressure during germplasm and these loci might be functional sites for the response to low phosphorus (Figure 5B). Our results are in agreement with the previous studies that LD decay could be different between 200 and 2000 bp and selective sweep in maize lines [52].…”

Section: Discussionsupporting

confidence: 94%

“…NAC transcription factors (TFs) contained different domains with at least 150 amino acids in the N-terminal region and these are responsible for DNA binding in the nucleus while the C-terminal region is related to transcriptional regulation and diverse functions [48]. In rice, Os04g0477300 (encoded NAC protein) was identified which is responsible for multiple processes including pathogenic diseases, B toxicity tolerance, boron and P deficiency [49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

“…Note: The letters represent nucleotides; letters represent the major alleles and underlined letters minor alleles. T/CK indicate low-P tolerant trait index; T represents P-deficient conditions; CK, normal-P conditions; MLM (Q+K), mixed linear model, population structure and kinship matrix; numerical numbers indicate the significant traits and -indicates data unavailable, ** indicates the -log10(0.01/41), *** indicate the -log10(0.001/41) according to the previously described method for correction test for p-values[52].…”

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confidence: 99%

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Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage

Sahito

Zhang

Zhong

et al. 2020

Plants

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Phosphorus (P) is an essential macroelement supporting maize productivity and low-P stress is a limiting factor of maize growth and yield. Improving maize plant tolerance to low P through molecular breeding is an effective alternative to increase crop productivity. In this study, a total of 111 diverse maize inbred lines were used to identify the favorable alleles and nucleotide diversity of candidate ZmNAC9, which plays an important role in response to low P and regulation in root architecture. A significant difference was found under low- and sufficient-P conditions for each of the 22 seedling traits, and a total of 41 polymorphic sites including 32 single nucleotide polymorphisms (SNPs) and 9 insertion and deletions (InDels) were detected in ZmNAC9 among 111 inbred lines. Among the 41 polymorphic studied sites, a total of 39 polymorphic sites were associated with 20 traits except for the dry weight of shoots and forks, of which six sites were highly significantly associated with a diverse number of low-P tolerant root trait index values by using a mixed linear model (MLM) at −log10 P = 3.61. In addition, 29 polymorphic sites under P-sufficient and 32 polymorphic sites under P-deficient conditions were significantly associated with a diverse number of seedling traits, of which five polymorphic sites (position S327, S513, S514, S520, and S827) were strongly significantly associated with multiple seedling traits under low-P and normal-P conditions. Among highly significant sites, most of the sites were associated with root traits under low-P, normal-P, and low-P trait index values. Linkage disequilibrium (LD) was strong at (r2 > 1.0) in 111 inbred lines. Furthermore, the effect of five significant sites was verified for haplotypes in 111 lines and the favorable allele S520 showed a positive effect on the dry weight of roots under the low-P condition. Furthermore, the expression pattern confirmed that ZmNAC9 was highly induced by low P in the roots of the P-tolerant 178 inbred line. Moreover, the subcellular localization of ZmNAC9 encoded by protein was located in the cytoplasm and nucleus. Haplotypes carrying more favorable alleles exhibited superior effects on phenotypic variation and could be helpful in developing molecular markers in maize molecular breeding programs. Taken together, the finding of this study might lead to further functions of ZmNAC9 and genes that might be involved in responses to low-P stress in maize.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage

Sahito

Zhang

Zhong

et al. 2020

Plants

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“…Moreover, stress tolerance in Pi deficiency is controlled by NAC and MAD TFs [ 72 , 106 ]. In particular, the gene ZmNAC134 has been associated with low Pi tolerance in maize seedlings [ 107 ]. In Arabidopsis, rice, and other plant species, MADS genes are also transcriptional responses to Pi deficiency and are important in mediating plant tolerance to Pi deficiency stress [ 4 , 108 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Profiling Reveals Potential Candidate Genes, Transcription Factors, and Biosynthetic Pathways for Phosphite Response in Potato (Solanum tuberosum L.)

Dormatey

Qin

Wang

et al. 2022

Genes

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The study was conducted with C31 and C80 genotypes of the potato (Solanum tuberosum L.), which are tolerant and susceptible to phosphite (Phi, H2PO3), respectively. To decipher the molecular mechanisms underlying tolerance and susceptibility to Phi in the potato, RNA sequencing was used to study the global transcriptional patterns of the two genotypes. Media were prepared with 0.25 and 0.50 mM Phi, No-phosphorus (P), and 1.25 mM (phosphate, Pi as control). The values of fragments per kilobase of exon per million mapped fragments of the samples were also subjected to a principal component analysis, grouping the biological replicates of each sample. Using stringent criteria, a minimum of 819 differential (DEGs) were detected in both C80-Phi-0.25_vs_C80-Phi-0.50 (comprising 517 upregulated and 302 downregulated) and C80-Phi-0.50_vs_C80-Phi-0.25 (comprising 302 upregulated and 517 downregulated) and a maximum of 5214 DEGs in both C31-Con_vs_C31-Phi-0.25 (comprising 1947 upregulated and 3267 downregulated) and C31-Phi-0.25_vs_C31-Con (comprising 3267 upregulated and 1947 downregulated). DEGs related to the ribosome, plant hormone signal transduction, photosynthesis, and plant–pathogen interaction performed important functions under Phi stress, as shown by the Kyoto Encyclopedia of Genes and Genomes annotation. The expressions of transcription factors increased significantly in C31 compared with C80. For example, the expressions of Soltu.DM.01G047240, Soltu.DM.08G015900, Soltu.DM.06G012130, and Soltu.DM.08G012710 increased under P deficiency conditions (Phi-0.25, Phi-0.50, and No-P) relative to the control (P sufficiency) in C31. This study adds to the growing body of transcriptome data on Phi stress and provides important clues to the Phi tolerance response of the C31 genotype.

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“…Phosphorus (P) is one of the essential nutrient elements for maize growth and has a vital influence on maize metabolism [1][2][3], because P is not only an energy carrier but also affects hormone synthesis, transportation, and metabolism in plants [4][5][6]. At present, the low P fertilizer utilization efficiency has become a global problem for high-yield crop production [7,8].…”

Section: Introductionmentioning

confidence: 99%

Content Patterns of Maize Endogenous Hormones and Grain Yield Influenced by Field Phosphorus Application

et al. 2023

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The content of plant endogenous hormones could be influenced by fertilization, which play an important regulatory role in plant growth and yield formation. However, the effects of phosphate (P) application rates on the dynamical changes in the exogenous hormone content in maize plants to support high-yield production are still unclear. An experiment with six P application rates was designed (P1, 0 kg ha−1; P2, 20 kg ha−1; P3, 40 kg ha−1; P4, 60 kg ha−1; P5, 80 kg ha−1; and P6, 100 kg ha−1), aimed at determining the distribution or biosynthesis of phytohormones in maize roots and leaves, and clarifying the role of P fertilization in the formation of phytohormones. The results showed that P fertilization significantly increased the content of IAA, by 51.57%, and significantly decreased the contents of ABA and GA3, by 18.92% and 19.13% on average compared to P1. The highest increase in IAA was 99.02%, and the highest decreases in ABA and GA3 were 32.30% and 26.85%, respectively, in the P6 treatment in maize roots. Meanwhile, an increase in the IAA (41.34%), SA (27.58%), and GA3 (37.36%) contents and a decrease in the ABA (19.18%) content in maize leaves were observed. The highest increases in IAA, GA3, and SA in the P6 treatment in maize leaves were 57.5%, 62.50%, and 48.57%, respectively, and the highest decrease in ABA was 28.05%. Meanwhile, different contents of endogenous hormones in maize roots and leaves were observed at various maize growth stages. The maize phenotypes and soil available P content were increased with P application as well. A Pearson’s correlation analysis showed that endogenous hormones were significantly correlated with maize phenotypes and yield. Our results confirmed that P fertilization could change the content of endogenous hormones in maize roots and leaves; P6 was the best treatment for improving the endogenous hormone contents in maize plants, P3 was the best for improving the maize grain yield. Overall, 40 kg ha−1 is recommended as the best P application rate under the experimental conditions, considering the economic cost and environmental effects.

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Identification, association, and expression analysis of ZmNAC134 gene response to phosphorus deficiency tolerance traits in maize at seedling stage

Cited by 5 publications

References 60 publications

Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage

Identification, Association of Natural Variation and Expression Analysis of ZmNAC9 Gene Response to Low Phosphorus in Maize Seedling Stage

Comparative Transcriptome Profiling Reveals Potential Candidate Genes, Transcription Factors, and Biosynthetic Pathways for Phosphite Response in Potato (Solanum tuberosum L.)

Content Patterns of Maize Endogenous Hormones and Grain Yield Influenced by Field Phosphorus Application

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