Identification and transcriptional analysis of SNARE vesicle fusion regulators in tomato (Solanum lycopersicum) during plant development and a comparative analysis of the response to salt stress with wild relatives

Salinas-Cornejo, Josselyn; Madrid-Espinoza, José; Ruíz-Lara, Simón

doi:10.1016/j.jplph.2019.153018

Cited by 24 publications

(28 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SNARE genes have been identified in the genome of the commercial tomato S. lycopersicum . Salinas-Cornejo and colleagues [ 18 ] identified 63 putative SNARE genes that segregate into five phylogenetic clades. Sl SNAP33.2 is predicted to localize on the plasma membrane as its paralogs do [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

“…Salinas-Cornejo and colleagues [ 18 ] identified 63 putative SNARE genes that segregate into five phylogenetic clades. Sl SNAP33.2 is predicted to localize on the plasma membrane as its paralogs do [ 18 ]. To confirm this location, the Sl SNAP33.2 CDS was cloned and fused to the GFP in an expression vector ( p35S::SlSNAP33.2::GFP ).…”

Section: Resultsmentioning

confidence: 99%

“…Three Qbc-SNAREs have been identified in the genomes of Solanum lycopersicum and its relative wild species ( S. chilense , S. pimpinellifolium , S. habrochaites , and S. pennellii ), namely SlSNAP33.1 , SlSNAP33.2 , and SlSNAP30 [ 18 ]. The transcripts accumulation of the three AtSNAP33 orthologs SlSNAP33.2, SchSNAP33.2 , and GsSNAP33 from Glycine soja increase during salt stress [ 18 , 19 ], suggest that SNAP33 orthologues play a role in the plant adaptation to abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

Salinas-Cornejo

Madrid-Espinoza

Verdugo

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

In plants, vesicular trafficking is crucial for the response and survival to environmental challenges. The active trafficking of vesicles is essential to maintain cell homeostasis during salt stress. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are regulatory proteins of vesicular trafficking. They mediate membrane fusion and guarantee cargo delivery to the correct cellular compartments. SNAREs from the Qbc subfamily are the best-characterized plasma membrane SNAREs, where they control exocytosis during cell division and defense response. The Solanum lycopersicum gene SlSNAP33.2 encodes a Qbc-SNARE protein and is induced under salt stress conditions. SlSNAP33.2 localizes on the plasma membrane of root cells of Arabidopsis thaliana. In order to study its role in endocytosis and salt stress response, we overexpressed the SlSNAP33.2 cDNA in a tomato cultivar. Constitutive overexpression promoted endocytosis along with the accumulation of sodium (Na+) in the vacuoles. It also protected the plant from cell damage by decreasing the accumulation of hydrogen peroxide (H2O2) in the cytoplasm of stressed root cells. Subsequently, the higher level of SlSNAP33.2 conferred tolerance to salt stress in tomato plants. The analysis of physiological and biochemical parameters such as relative water content, the efficiency of the photosystem II, performance index, chlorophyll, and MDA contents showed that tomato plants overexpressing SlSNAP33.2 displayed a better performance under salt stress than wild type plants. These results reveal a role for SlSNAP33.2 in the endocytosis pathway involved in plant response to salt stress. This research shows that SlSNAP33.2 can be an effective tool for the genetic improvement of crop plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

Salinas-Cornejo

Madrid-Espinoza

Verdugo

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…We identified several Syntaxin family proteins (ClCG02G015160, ClCG07G004070, ClCG08G012870, ClCG09G005420, ClCG10G004910, ClCG10G018250) and Golgi SNAP receptor complex member 1 , target SNARE coiled-coil domain , vesicle-trafficking SEC22b , and vesicle transport v-SNARE 11-like genes that were up-regulated. Several studies [ 69 , 70 , 71 , 72 ] have reported the functional role of SNARE interactions in salt stress, justifying the significance of SNARE proteins in salt stress-induced responses in watermelon.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Short-Term Salt Stress Response in Watermelon Seedlings

Song

Joshi²,

Joshi³

2020

IJMS

View full text Add to dashboard Cite

Watermelon (Citrullus lanatus L.) is a widely popular vegetable fruit crop for human consumption. Soil salinity is among the most critical problems for agricultural production, food security, and sustainability. The transcriptomic and the primary molecular mechanisms that underlie the salt-induced responses in watermelon plants remain uncertain. In this study, the photosynthetic efficiency of photosystem II, free amino acids, and transcriptome profiles of watermelon seedlings exposed to short-term salt stress (300 mM NaCl) were analyzed to identify the genes and pathways associated with response to salt stress. We observed that the maximal photochemical efficiency of photosystem II decreased in salt-stressed plants. Most free amino acids in the leaves of salt-stressed plants increased many folds, while the percent distribution of glutamate and glutamine relative to the amino acid pool decreased. Transcriptome analysis revealed 7622 differentially expressed genes (DEGs) under salt stress, of which 4055 were up-regulated. The GO analysis showed that the molecular function term “transcription factor (TF) activity” was enriched. The assembled transcriptome demonstrated up-regulation of 240 and down-regulation of 194 differentially expressed TFs, of which the members of ERF, WRKY, NAC bHLH, and MYB-related families were over-represented. The functional significance of DEGs associated with endocytosis, amino acid metabolism, nitrogen metabolism, photosynthesis, and hormonal pathways in response to salt stress are discussed. The findings from this study provide novel insights into the salt tolerance mechanism in watermelon.

show abstract

“…Seed germination is a very important stage during the processes of growth and development in plants, and is accompanied by a series of physiological and molecular changes, including enzyme activities, endogenous phytohormone contents, gene expression levels, and protein types [24]. This process is affected by plant growth regulators, water, and temperature, among others [24][25][26][27]. The plant growth regulators are divided into ve classes, one of them being Gibberellin (GA), which is a diterpenoid compound, and the most active arti cially synthesized hormone [28,29].…”

Section: Discussionmentioning

confidence: 99%

Exogenous Gibberellin Promotes the Germination of Phellodendron Chinense Schneid Seeds by Regulating the Expression of Phytohormone Related Genes and Anabolic Proteins

Sun

Zhang

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Phellodendron chinense Schneid is an important Chinese herb that contains berberine, phellodendrine, palmatine, and medicinal compounds. The germination rate of Phellodendron chinense Schneid seeds is lower after storage, and the exogenous gibberellin3 (GA3) hormone promotes seed germination, but the mechanism is not cleared. Results: Exogenous GA3 hormone promoted germination of Phellodendron chinense Schneid seeds, elevated germination rates. It also increased the levels of activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Moreover, it enhanced the contents of berberine and endogenous GA3, and increased the expression levels of Pc(S)-GA2ox, Pc(S)-GA3ox, and Pc(S)-THBO. However, it reduced the expression level of Pc(S)-ABI5. Furthermore, exogenous GA3 up-regulated the protein levels of DNA guides RNA polymerase β'-subunits, Coffee coenzyme A oxymethyl transferase, and Histone H1. Conclusion: These findings indicated that exogenous GA3 promoted germination of Phellodendron chinense Schneid seeds by regulating the expression levels of phytohormone related genes and anabolic proteins.

show abstract

Identification and transcriptional analysis of SNARE vesicle fusion regulators in tomato (Solanum lycopersicum) during plant development and a comparative analysis of the response to salt stress with wild relatives

Cited by 24 publications

References 68 publications

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

Transcriptomic Analysis of Short-Term Salt Stress Response in Watermelon Seedlings

Exogenous Gibberellin Promotes the Germination of Phellodendron Chinense Schneid Seeds by Regulating the Expression of Phytohormone Related Genes and Anabolic Proteins

Contact Info

Product

Resources

About