Identification and sequencing of Date-SRY Gene: A novel tool for sex determination of date palm (Phoenix dactylifera L.)

Abstract

Dioecism has always been an issue in many plant species with its numerous disadvantages, especially in woody trees such as date palms. As one of the most important crops in the Middle Eastern countries, researchers are having problems identifying of sex of the plant in its early stages of development. Hence, proper population stands in the male: female ratio for maintenance is almost impossible in the field for better production. In this study, sex determination of date palm (Phoenix dactilyfera L.) were identified in regions of the Y chromosome (Date-SRY) gene, the pivotal gene that initiates sex determination, using a new technique and thus an economically desirable objective, which will significantly impact profits in seed based cultivations. Partial sequences of the Date-SRY were taken and amplified by nested polymerase chain reaction (PCR). According to the results, the exact sex of date palm was identified in all the tested plants, while amplified regions of the Date-SRY gene closely matched with the human and papaya sequences. In addition, a primer pair was designed to amplify the sequences of the SRY-date gene with confidence that it will identify male date palms. These primer sequences include SRY-date Forward 5′- cggccctctaagtatctgtgcgcaacg-3′ (SRY-date F) and the SRY-date Reverse 5′- gtttgcacttcgaagcagag-3′ (SRY-date R). The complete sequence of the DNA has been registered and deposited in GenBank (BankIt1598036 DPSRY1 KC577225 thenKJ873056).

DNA Fingerprinting of Sex in Jojoba (Simmondsia chinensis) Grown under the Semi-arid Conditions of Sudan

“…Although Sudan is not within the belt where jojoba is grown naturally, the successful establishment and production of seeds from the crop that grown at different parts in Sudan, especially at Arkwakeet, indicates that the country could have a real potential investment possibilities for production of seeds and oil if the problem associated with identification of male and female plants at the early stage of development is solved. DNA markers have been extensively used in sex determination of dioecious plant such as Phoenix dactylifera [20], Carica papaya [21] and Simmondsia chinensis [14,16,17,18]. In the present study, the RAPD primer OPG5 1400 ) was in variance to the result of [14] to amplify a unique male-specific fragment as shown in Figure1.…”

Towards sex identification of Asian Palmyra palm (Borassus flabelliferL.) by DNA fingerprinting, suppression subtractive hybridization andde novotranscriptome sequencing

“…This problem has recently been solved by the availability of date palm genomes (Al-Dous et al, 2011), verification of sex determination loci (Cherif et al, 2013) and construction of genetic map (Mathew et al, 2014), validating that date palm has a homomorphic XY chromosome system. This sex chromosome is currently being used to develop precise sex-specific markers for specific varieties and populations of date palm (Mohei et al, 2017; Ali et al, 2018; Intha & Chaiprasart, 2018). Thus, it is potential that genome sequencing of Asian Palmyra palm could help us verify the sex determination region and, perhaps, the sex determination system in Asian Palmyra palm.…”

Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

“…There is a lack of information regarding the genetics of date palm. Several cytological studies have showed heteromorphic chromosome pair in male plants and different appearance of sexual chromosomes DOI: 10.2478/arls-2019-0005 ARLS, 3(1), 2019, [17][18][19][20][21][22] in date palm [7]. Although the results were useful to distinguish between male and female date palm, and recently DNA fingerprinting techniques have been considered as reliable and quick methods for sex determination in date palm plants [8].…”

“…RAPD . Date palm genome has a region linked to gender which had been identified a few years ago, in this region there are sex determination sequences [21]. Date palm is heterogametic sex (XY) in male over and above there is significant polymorphism in the alleles of male and female which can be helpful in the process of sex determination [22].…”

“…), 2019,[17][18][19][20][21][22] carried out in 20 μl reaction mixture containing 4μl of 5x HOT FIREPol® Blend Master Mix Ready, 20 picmole primers, 2 μl of genomic DNA and 13.2 μl dd H 2 O. The PCR reactions were conducted for an initial denaturation at °C for 2 min, followed by…”