Jojoba cultivation and production face the challenge of establishing ways to identify the sex at early stage of plant growth. The present study was carried out to identify sex of jojoba at the seedling stage under Sudan condition. Two DNA markers, ISSR (UBC807) and RAPD (OPG-5), were used for sex identification of jojoba genotypes: two known male and females genotypes and four unknown genotypes. ISSR marker, UBC807 was successfully amplified a unique male-specific band at 1200 bp, while RAPD marker, OPG-5 could not amplify a unique band within jojoba sex. The result clearly indicates that ISSR-UBC807 marker can be used for sex identification of jojoba at seedlings stage, a finding that could make the commercial cultivation and production of jojoba possible in Sudan.
The knowledge of genetic diversity of a crop like Jojoba, a commercially grown plant to produce unique liquid oil from its seed, is essential in a breeding program aiming to the development of elite genotypesfor large-scale production. This study was carried out to evaluate the genetic diversity of 25 female jojoba genotypes growing in Arkaweet Area, East Sudan, using ISSR Markers. Genomic DNA was extracted from leaves of thestudied genotypes and quantified for PCR amplification. Five ISSR primers were used for PCR amplification to investigate the genetic diversity among the female jojoba genotypes. ISSR primers showed high polymorphic pattern and produced a total of 54 polymorphic bands with an average of 10.8 polymorphic bands per primer. The average of polymorphic information content (PIC) value of 0.37 indicates moderate polymorphism. The geneticdiversity ranged from 0.01 to 0.85. The results indicate the effectiveness of using ISSR molecular markers to reveal genetic variability among the studied female jojoba genotypes, a finding that could be used for development ofelite jojoba genotypes, which provide potential for large-scale jojoba production in Sudan.
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