Identification and regulation of host genes related to <i>Rice stripe virus</i> symptom production

Shi, Bingbin; Lin, Lin; Wang, Shihui; Guo, Qin; Zhou, Hong; Rong, Lingling; Li, Junmin; Peng, Jiejun; Lu, Yuwen; Zheng, Hongying; Yang, Yong; Chen, Zhuo; Zhao, Jinping; Jiang, Tong; Song, Baoan; Chen, Jianping; Yan, Fei

doi:10.1111/nph.13699

Cited by 72 publications

(90 citation statements)

References 53 publications

(79 reference statements)

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“…Among these DEGs, chloroplast‐ and photosynthesis‐related genes were overrepresented. Several studies have reported that chloroplast‐related or photosynthesis genes are significantly changed during plant virus infection, and that the abnormal expression of these genesis is directly related to symptom development in plant leaves (Bhat et al ., ; Cheng et al ., ; Reuveni et al ., ; Shi et al ., ). As shown in Table S4, photosynthesis‐related antenna proteins, Chl a‐b binding protein and carbon fixation were markedly down‐regulated after virus infection.…”

Section: Discussionmentioning

confidence: 97%

Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice

Zhang

Sun³

et al. 2016

New Phytologist

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129

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Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. We used a high-throughput-sequencing approach to compare the global gene expression of Rice black-streaked dwarf virus (RBSDV)-infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid (JA) pathway was induced while the brassinosteroid (BR) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole (BRZ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide (BL) treatment increased RBSDV infection. Infection studies using coi1-13 and Go mutants demonstrated JA-mediated resistance and BR-mediated susceptibility to RBSDV infection. A mixture of MeJA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1. Collectively, our results reveal that JA-mediated defense can suppress the BR-mediated susceptibility to RBSDV infection.

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Section: Discussionmentioning

confidence: 97%

Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice

Zhang

Sun³

et al. 2016

New Phytologist

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129

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“…The downregulation of CHLI protein, which is involved in chlorophyll biosynthesis, leads to the loss of chlorophyll production and severer chlorosis in a host-specific manner, which is dependent on the CHLI gene sequence [26,27]. Besides the chlorosis, leaf twisting symptom has been attributed to the v-siRNA-mediated silencing of eIF4A in RSV-infected N. benthamiana [25].…”

Section: Introductionmentioning

confidence: 99%

Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 90

et al. 2017

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Chlorosis is one of the most common symptoms of plant diseases, including those caused by viruses and viroids. Recently, a study has shown that Peach latent mosaic viroid (PLMVd) exploits host RNA silencing machinery to modulate the virus disease symptoms through the silencing of chloroplast-targeted heat shock protein 90 (Hsp90C). To understand the molecular mechanisms of chlorosis in this viroid disease, we established an experimental system suitable for studying the mechanism underlying the chlorosis induced by the RNA silencing of Hsp90C in transgenic tobacco. Hairpin RNA of the Hsp90C-specific region was expressed under the control of a dexamethasone-inducible promoter, resulted in the silencing of Hsp90C gene in 2 days and the chlorosis along with growth suppression phenotypes. Time course study suggests that a sign of chlorosis can be monitored as early as 2 days, suggesting that this experimental model is suitable for studying the molecular events taken place before and after the onset of chlorosis. During the early phase of chlorosis development, the chloroplast-and photosynthesis-related genes were downregulated. It should be noted that some pathogenesis related genes were upregulated during the early phase of chlorosis in spite of the absence of any pathogen-derived molecules in this system.

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“…The first report of this phenomenon was the targeting of the chlorophyll biosynthetic gene (CHLI) of Nicotiana by siRNAs derived from CMV Y-satellite, resulting in the yellowing of the plant (Shimura et al, 2011; Smith et al, 2011). It has also recently been shown that the eukaryotic translation initiation factor 4A (eIF4A) of Nicotiana benthamiana can be targeted by siRNA derived from Rice stripe virus (RSV), resulting in leaf-twisting and stunting (Shi et al, 2016). These results indicate the complicated function of vsiRNAs during virus-host interaction.…”

Section: Introductionmentioning

confidence: 99%

Different Virus-Derived siRNAs Profiles between Leaves and Fruits in Cucumber Green Mottle Mosaic Virus-Infected Lagenaria siceraria Plants

Zheng

Zhang

et al. 2016

Front. Microbiol.

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RNA silencing is an evolutionarily conserved antiviral mechanism, through which virus-derived small interfering RNAs (vsiRNAs) playing roles in host antiviral defense are produced in virus-infected plant. Deep sequencing technology has revolutionized the study on the interaction between virus and plant host through the analysis of vsiRNAs profile. However, comparison of vsiRNA profiles in different tissues from a same host plant has been rarely reported. In this study, the profiles of vsiRNAs from leaves and fruits of Lagenaria siceraria plants infected with Cucumber green mottle mosaic virus (CGMMV) were comprehensively characterized and compared. Many more vsiRNAs were present in infected leaves than in fruits. vsiRNAs from both leaves and fruits were mostly 21- and 22-nt in size as previously described in other virus-infected plants. Interestingly, vsiRNAs were predominantly produced from the viral positive strand RNAs in infected leaves, whereas in infected fruits they were derived equally from the positive and negative strands. Many leaf-specific positive vsiRNAs with lengths of 21-nt (2058) or 22-nt (3996) were identified but only six (21-nt) and one (22-nt) positive vsiRNAs were found to be specific to fruits. vsiRNAs hotspots were only present in the 5′-terminal and 3′-terminal of viral positive strand in fruits, while multiple hotspots were identified in leaves. Differences in GC content and 5′-terminal nucleotide of vsiRNAs were also observed in the two organs. To our knowledge, this provides the first high-resolution comparison of vsiRNA profiles between different tissues of the same host plant.

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Identification and regulation of host genes related to Rice stripe virus symptom production

Cited by 72 publications

References 53 publications

Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice

Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice

Inducible transgenic tobacco system to study the mechanisms underlying chlorosis mediated by the silencing of chloroplast heat shock protein 90

Different Virus-Derived siRNAs Profiles between Leaves and Fruits in Cucumber Green Mottle Mosaic Virus-Infected Lagenaria siceraria Plants

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