Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to <i>Rice black streaked dwarf virus</i> infection in rice

He, Yuqing; Zhang, Hehong; Sun, Zongtao; Li, Junmin; Hong, Gaojie; Zhu, Qisong; XueBiao, Zhou; MacFarlane, Stuart A.; Yan, Fei; Chen, Jianping

doi:10.1111/nph.14376

Cited by 138 publications

(155 citation statements)

References 55 publications

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“…Our previous results showed that the JA and BR pathways were altered in opposite directions in response to RBSDV infection (He et al, ). Further investigations of the high‐throughput sequencing data showed that ABA biosynthetic genes OsNCED3 and OsABA8ox were significantly reduced in RBSDV‐infected rice in comparison to that in mock plants (Table S2).…”

Section: Resultsmentioning

confidence: 94%

“…Previous reports indicated that the JA pathway and ROS levels play important roles in resistance to RBSDV (He et al, ; Wu et al, ; Zhang et al, ). We therefore examined the interaction between ABA, the JA pathway, and ROS levels.…”

Section: Resultsmentioning

confidence: 96%

“…RBSDV was inoculated as described previously (He et al, ; Zhou et al, ). Briefly, there were at least three biological replicates of each treatment, and for each replicate, at least 40 seedlings of the wild type or mutant rice were sown.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study of RNA transcripts using high‐throughput sequencing, the jasmonate (JA) and brassinosteroid (BR) pathways were inversely regulated in response to RBSDV infection, and JA‐mediated plant defence was shown to suppress BR‐mediated susceptibility to RBSDV (He et al, ). The key ABA biosynthetic genes OsNCED3 and OsABA8ox were significantly reduced in response to viral infection, but our understanding of the interaction between ABA and RBSDV is limited.…”

Section: Introductionmentioning

confidence: 99%

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Abscisic acid negatively modulates plant defence against rice black‐streaked dwarf virus infection by suppressing the jasmonate pathway and regulating reactive oxygen species levels in rice

Xie

Zhang

et al. 2018

Plant Cell & Environment

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Abscisic acid (ABA) plays a multifaceted role in plant immunity and can either increase resistance or increase susceptibility to some bacterial and fungal pathogens depending on the pathosystem. ABA is also known to mediate plant defence to some viruses. In this study, the relationship between the ABA pathway and rice black-streaked dwarf virus (RBSDV) was investigated in rice. The expression of ABA pathway genes was significantly reduced upon RBSDV infection. Application of exogenous hormones and various ABA pathway mutants revealed that the ABA pathway plays a negative role in rice defence against RBSDV. Exogenous hormone treatment and virus inoculation showed that ABA inhibits the jasmonate-mediated resistance to RBSDV. ABA treatment also suppressed accumulation of reactive oxygen species by inducing the expression of superoxidase dismutases and catalases. Thus, ABA modulates the rice-RBSDV interaction by suppressing the jasmonate pathway and regulating reactive oxygen species levels. This is the first example of ABA increasing susceptibility to a plant virus.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Abscisic acid negatively modulates plant defence against rice black‐streaked dwarf virus infection by suppressing the jasmonate pathway and regulating reactive oxygen species levels in rice

Xie

Zhang

et al. 2018

Plant Cell & Environment

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“…In pathogens defense, plants produce a complicated mixture of SA, JA, and ABA to modulate plant defense response to invading pathogens (Kunkel and Brooks, 2002; Chen et al, 2013; He et al, 2017). CaRDR1 was response to multiple plant hormones in this study (Figure 3), especially induced by SA like other RDR1 orthologs (Yang et al, 2004; Hunter et al, 2013; Liao et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

CaRDR1, an RNA-Dependent RNA Polymerase Plays a Positive Role in Pepper Resistance against TMV

Qin

Zhang

et al. 2017

Front. Plant Sci.

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RNA silencing functions as a major natural antiviral defense mechanism in plants. RNA-dependent RNA polymerases (RDRs) that catalyze the synthesis of double-stranded RNAs, are considered as a fundamental element in RNA silencing pathways. In Arabidopsis thaliana, RDR1, 2 and 6 play important roles in anti-viral RNA silencing. Expression of RDR1 can be elevated following plant treatment with defense hormones and virus infection. RDR1 has been studied in several crop species, but not in pepper (Capsicum annuum L.). Here, a RDR1 gene was isolated from Capsicum annuum L., designated as CaRDR1. The full-length cDNA of CaRDR1 was 3,351 bp, encoding a 1,116-amino acid protein, which contains conserved regions, such as the most remarkable motif DLDGD. The transcripts of CaRDR1 could be induced by salicylic acid (SA), abscisic acid (ABA), H2O2, and tobacco mosaic virus (TMV). Silencing of CaRDR1 in pepper resulted in increased susceptibility to TMV as evident by severe symptom, increased of TMV-CP transcript, higher malondialdehyde (MDA) content and lower antioxidant enzymes activities compared with that of control plants. CaRDR1-overexpressing in Nicotiana benthamiana showed mild disease symptom and reduced TMV-CP transcripts than that of empty vector (EV) following TMV inoculation. The RNA silencing related genes, including NbAGO2, NbDCL2, NbDCL3, and NbDCL4 elevated expression in overexpressed plants. Alternative oxidase (AOX), the terminal oxidase of the cyanide (CN)-resistant alternative respiratory pathway, catalyze oxygen-dependent oxidation of ubiquinol in plants. It has an important function in plant defense against TMV. In addition, CaRDR1 overexpression promoted the expression of NbAOX1a and NbAOX1b. In conclusion, these results suggest that CaRDR1 plays a positive role in TMV resistance by regulating antioxidant enzymes activities and RNA silencing-related genes expression to suppress the replication and movement of TMV.

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Pepper vein yellow virus P0 protein triggers NbHERC3, NbBax, and NbCRR mediated hypersensitive response

OuYang,

Wang,

Luo

et al. 2024

J Basic Microbiol

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P0 proteins encoded by the pepper vein yellow virus (PeVYV) are pathogenic factors that cause hypersensitive response (HR). However, the host gene expression related to PeVYV P0‐induced HR has not been thoroughly studied. Transcriptomic technology was used to investigate the host pathways mediated by the PeVYV P0 protein to explore the molecular mechanisms underlying its function. We found 12,638 differentially expressed genes (DEGs); 6784 and 5854 genes were significantly upregulated and downregulated, respectively. Transcriptomic and reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR) analyses revealed that salicylic acid (SA) and jasmonic acid (JA) synthesis‐related gene expression was upregulated, and ethylene synthesis‐related gene expression was downregulated. Ultrahigh performance liquid chromatography‐tandem mass spectrometry was used to quantify SA and JA concentrations in Nicotiana benthamiana, and the P0 protein induced SA and JA biosynthesis. We then hypothesized that the pathogenic activity of the P0 protein might be owing to proteins related to host hormones in the SA and JA pathways, modulating host resistance at different times. Viral gene silencing suppression technology was used in N. benthamiana to characterize candidate proteins, and downregulating NbHERC3 (Homologous to E6‐AP carboxy‐terminus domain and regulator of choromosome condensation‐1 dmain protein 3) accelerated cell necrosis in the host. The downregulation of NbCRR reduced cell death, while that of NbBax induced necrosis and curled heart leaves. Our findings indicate that NbHERC3, NbBax, and NbCRR are involved in P0 protein‐driven cell necrosis.

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Jasmonic acid‐mediated defense suppresses brassinosteroid‐mediated susceptibility to Rice black streaked dwarf virus infection in rice

Cited by 138 publications

References 55 publications

Abscisic acid negatively modulates plant defence against rice black‐streaked dwarf virus infection by suppressing the jasmonate pathway and regulating reactive oxygen species levels in rice

Abscisic acid negatively modulates plant defence against rice black‐streaked dwarf virus infection by suppressing the jasmonate pathway and regulating reactive oxygen species levels in rice

CaRDR1, an RNA-Dependent RNA Polymerase Plays a Positive Role in Pepper Resistance against TMV

Pepper vein yellow virus P0 protein triggers NbHERC3, NbBax, and NbCRR mediated hypersensitive response

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