Identification and quantification of tylosin in animal feed by liquid chromatography combined with electrospray ionisation mass spectrometry

Przenioslo-Siwczynska, M.; Grelik, A.; Kwiatek, K.

doi:10.2478/jvetres-2020-0031

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…Additionally, antimicrobials, especially those with agronomical applications, are routinely scrutinized in medicated and non-medicated feed (as they are used for prophylaxis, methaphylaxis, and growth promotion) [ 313 , 321 , 322 ], in animal tissue [ 323 ] and other essential commodities (e.g., eggs, vegetables) [ 312 , 324 , 325 , 326 ]. LC-MS has also a widespread application in the determination of pesticide residues in foods, as the accumulation of these substances may cause toxic and allergic effects for health as a result of the consumption of contaminated products [ 327 , 328 ].…”

Section: Selected Instrumental Techniques and Their Applicationsmentioning

confidence: 99%

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Artavia

Cortés-Herrera

Granados-Chinchilla³

2021

Foods

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This review presents an overall glance at selected instrumental analytical techniques and methods used in food analysis, focusing on their primary food science research applications. The methods described represent approaches that have already been developed or are currently being implemented in our laboratories. Some techniques are widespread and well known and hence we will focus only in very specific examples, whilst the relatively less common techniques applied in food science are covered in a wider fashion. We made a particular emphasis on the works published on this topic in the last five years. When appropriate, we referred the reader to specialized reports highlighting each technique’s principle and focused on said technologies’ applications in the food analysis field. Each example forwarded will consider the advantages and limitations of the application. Certain study cases will typify that several of the techniques mentioned are used simultaneously to resolve an issue, support novel data, or gather further information from the food sample.

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Section: Selected Instrumental Techniques and Their Applicationsmentioning

confidence: 99%

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Artavia

Cortés-Herrera

Granados-Chinchilla³

2021

Foods

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“…In scientific publications, methods can be found of quantitative analysis of single substances in medicated feed (e.g. sulfaguanidine, tylosin, tiamulin, amoxicillin, florfenicol and sulfamethazine) (5,10,11,18,20,23,25,26), and of analysis of several compounds belonging to the same chemical group (sulfonamides, phenicols, β-lactams and tetracyclines) (1, 2, 4, 8, 12-17, 21, 22). However, few developed analytical methods exist for several antibacterial substances belonging to different chemical groups (19).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulfonamides, trimethoprim, amoxicillin and tylosin in medicated feed by high performance liquid chromatography with mass spectrometry

Patyra,

Kwiatek

2024

Journal of Veterinary Research

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Introduction The article presents a rapid and simple analytical procedure for determination of four sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine and sulfamethoxazole), trimethoprim, tylosin and amoxicillin in animal medicated feed. Material and Methods Eighteen medicated feed samples were analysed for active substances. The analytical protocol used a mixture of acetonitrile and 0.05 M phosphoric buffer, pH 4.5 for the extraction of seven antibacterial substances. After extraction, the samples were diluted in Milli-Q water and analysed by liquid chromatography with mass spectrometry. The developed procedure was subjected to validation in terms of linearity, selectivity, limits of quantification and determination, repeatability, reproducibility and uncertainty. Results The validation of the method was carried out in accordance with the criteria set out in Commission Implementing Regulation (EU) 2021/808 and ICH guidelines. This method provided average recoveries of 90.8 to 104.5% with coefficients of variation for repeatability and reproducibility in the ranges of 3.2–6.9% and 5.2–8.3%, respectively for all analysed antibacterial substances. The limit of detection and limit of quantification for all seven analytes ranged from 5.4 mg/kg to 48.3 mg/kg and from 10.4 mg/kg to 119.3 mg/kg, respectively. The uncertainty of the method depending on the compound varied from 14.0% to 24.0%. The validated method was successfully applied to the 18 medicated feeds. Conclusion The developed method can be successfully used to routinely control the content and homogeneity of seven antibacterial substances in medicated feed.

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“…The wide use of tylosin antibiotic from the macrolide series makes it crucial to regulate their concentration in both dosage forms and biological liquids [6]. Currently, techniques like liquid chromatography [7], high performance liquid chromatography (HLPC) [8] [9] [10] and spectrophotometric methods [11] are employed to identify macrolide antibiotics. Tylosin (TYS) is White to buff-colored powder with a mild musty odor; its melting point is "128˚C -132˚C" and its solubility in water 5 mg/mL at 25˚C and Freely soluble in methanol.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Physicochemical Studies for Novel Bioactive Metal Complexes of Macrolide Antibiotic Tylosin

Ali¹,

Elasala²,

Mahrous³

et al. 2022

MSA

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Tylosin is a well-established antibiotic that has been widely employed in human and veterinary medicines. It can act as a potential ligand binding metal ions due to various donor atoms in the structure. Our study on the complexation of various metal ions with tylosin ligand revealed that they preferably coordinate with mycaminose fragment to establish Novel trends complexes. Tylosin ligand (TYS) behaves as bidentate for complexation with different metal ions such as Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II) and Zn(II). Various essential metal complexes of tylosin were synthesized and characterized by techniques such as UV, IR, Elemental analysis, magnetic susceptibility and ESR spectra of Cu(II) complex. These techniques are used to know their geometries and mode of bonding, with stoichiometry, 2:2 (M:L). Thermal analysis (TGA and DTA) of ligands and their metal complexes were carried out to distinguish between the coordinate and hydrate solvents and to estimate the stability ranges, peak temperatures. The thermodynamic parameters, such as activation energy (ΔE*), the enthalpy of activation (ΔH*), entropy of activation (ΔS*) and Gibbs free energy (ΔG*) are calculated and discussed. Some tylosin complexes show higher activity than tylosin for some bacterial and fungal strains. Low concentration value of minimum inhibitory concentration (MIC) results is 15.625 µg/ml for both complexes [Zn 2 (TYS) 2 Cl 2 (H 2 O) 4 ]•25H 2 O and [Cu 2 (TYS) 2 Cl 2 (H 2 O) 4 ]•25H 2 O with B. cereus genus maybe a valuable data used to produce novel therapeutic agent. This study constitutes several essential aspects for future research on tylosin metal complexes as antibacterial assessment and as potential medicinal agents.

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Identification and quantification of tylosin in animal feed by liquid chromatography combined with electrospray ionisation mass spectrometry

Cited by 6 publications

References 21 publications

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Simultaneous determination of sulfonamides, trimethoprim, amoxicillin and tylosin in medicated feed by high performance liquid chromatography with mass spectrometry

Synthesis and Physicochemical Studies for Novel Bioactive Metal Complexes of Macrolide Antibiotic Tylosin

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