Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MSn

Fischer, Ulrike A.; Carle, Reinhold; Kammerer, Dietmar R.

doi:10.1016/j.foodchem.2010.12.156

Cited by 720 publications

(673 citation statements)

References 47 publications

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“…Also, the result of this study was parallel with Anahita et al 10 that pomegranate seed + juice had the highest total antioxidant activity (47.00 ± 5.50) than two other parts (seed and juice), separately. On the other hand, Mphahlele et al 11 Akhavan et al 12 and Fischer et al 13 had identified the individual AC in pomegranate extract, and the result was parallel with the present study. However, undetectable AC and its derivatives in the juices among those studies and present study are contrary to each other, and this may lead to several reasons.…”

Section: Discussionsupporting

confidence: 90%

Quantitative and Optimization of Anthocyanin Extracted from Pomegranate (Punica Granatum) Extract by High-Performance Liquid Chromatography (HPLC)

Ridzwan¹,

Noratiqah²,

Amin³

et al. 2018

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Objective: P. granatum is one of the oldest edible fruits of tropical and subtropical regions. This fruit had high antioxidant contained by hydrolysable tannins and anthocyanin compounds that give many health benefit properties. This study aims to quantify and optimized anthocyanin from P. granatum extract. Methods: A total of 50g of the flesh was soaked into two different polar solvents; water and 50% ethanol within a ratio of 1:10; w/v for 24-hr. Then, three different methods of extraction were done and test each with HPLC analytical, respectively. Results: The validated method proved to be linear in the range of 5 -30 ug/mL and with LOD and LOQ determined respectively for Cy3, Cy3, 5, Pg3, and Pg3, 5. The method also shows recovery (%) close to 100 when accuracy was accessed. For samples, blender water extract had a higher composition of Cy3, Cy3, 5 and Pg3, 5 (22.77 ± 8.82 mg/100 g e.p; 25.36 ± 9.95 mg/100 g e.p; 11.16 ± 5.85 mg/100 g e.p) content as compared to other. Conclusion: As a conclusion, the present methodology proved to be capable of detecting and quantifying Cy3, Cy3, 5, Pg3, Pg3, 5 in a single run. Also, comparatively the composition of each AC detected in blender water extract is significantly higher in value than the other methods. It should regard as a valuable source of antioxidant with the potential used for health benefits properties worldwide.

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Section: Discussionsupporting

confidence: 90%

Quantitative and Optimization of Anthocyanin Extracted from Pomegranate (Punica Granatum) Extract by High-Performance Liquid Chromatography (HPLC)

Ridzwan¹,

Noratiqah²,

Amin³

et al. 2018

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“…In the present study, the two phenolic compounds from PG II fraction of P. granatum were identified by the interpretation of their fragmentation patterns obtained from mass spectra and retention times of peaks (Figure 1.4). The peak of compound 1 at retention time 1.47 min had molecular weight [M-H]-at m/z 169 which was identified as gallic acid and peak of compound 2 at retention time 2.05 min had molecular weight [M-H]-at m/z 179 which was also identified as caffeic acid ( Figure 1.4 20 . According to Borochov-Neori et al, (2011), some phenolic compounds were extremely dependent on the climate conditions and the proportion of phenolic compounds increased with seasonal warming 22 .…”

Section: Liquid Chromatography Mass Spectrometry (Lc-ms) Analysismentioning

confidence: 99%

Chromatographic Determination of Phenolic Profile From Punica Granatum Fruit Peels

Saxena¹,

Sharma²,

Nandy³

2017

Int. Res. J. Pharm.

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Polyphenols are the important active compounds present in the fruits peels of Punica granatum. The objectives of this study were to isolate and identify the phenolic compounds by UV-spectrophotometry, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography -mass spectrometry (LC-MS) methods for qualitative and quantitative analyses of phenolic compounds in fruit peels of P. granatum. For separation of bioactive compounds, the crude ethanolic extracts of P. granatum were fractionated by using column chromatography. These fractions were coded as PG I, PG II, PG III, PG IV and PG V for P. granatum. These fractions were analyzed by thin layer chromatography (TLC) and showed that PG II fraction had an interested compound. The highest total phenolic and flavonoid content were observed in the PG II fraction of P. granatum (617 ± 0.017 mg/g and 546.33 ± 0.032 mg/g respectively) in comparison to other fractions. The phenolic compounds gallic acid and caffeic have been identified in the PG II fraction of P. granatum by using different chromatographic techniques i.e. TLC, HPLC, LC-MS analysis. The overall results of the present studies were indicated that PG II fraction of P. granatum was a good source of phenolic compounds. The present analytical study proved a potential application to identify and quantify the phenolic compounds in the plant fractions. Hence, it could be pursued further for obtaining phytomedicine.

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“…A romã (Punica granatum L.) atualmente é bastante requisitada pelas indústrias farmacêutica e de cosmético, tendo sido comprovada a presença de punicalagina, um tanino hidrolisável, com propriedades anti-inflamatória e antioxidante (GONZÁLEZ-MOLINA et al, 2009), principalmente na casca e no mesocarpo do fruto (FISCHER et al, 2011).…”

Section: Introductionunclassified

POTENCIAL FISIOLÓGICO DE DIÁSPOROS DE Punica granatum L. APÓS FERMENTAÇÃO EM ÁCIDO GIBERÉLICO

Pinto¹,

Araújo²,

Barbosa³

et al. 2016

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Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MSn

Cited by 720 publications

References 47 publications

Quantitative and Optimization of Anthocyanin Extracted from Pomegranate (Punica Granatum) Extract by High-Performance Liquid Chromatography (HPLC)

Quantitative and Optimization of Anthocyanin Extracted from Pomegranate (Punica Granatum) Extract by High-Performance Liquid Chromatography (HPLC)

Chromatographic Determination of Phenolic Profile From Punica Granatum Fruit Peels

POTENCIAL FISIOLÓGICO DE DIÁSPOROS DE Punica granatum L. APÓS FERMENTAÇÃO EM ÁCIDO GIBERÉLICO

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