Chromatographic Determination of Phenolic Profile From Punica Granatum Fruit Peels

Abstract

Polyphenols are the important active compounds present in the fruits peels of Punica granatum. The objectives of this study were to isolate and identify the phenolic compounds by UV-spectrophotometry, thin layer chromatography (TLC), high-performance liquid chromatography (HPLC) and liquid chromatography -mass spectrometry (LC-MS) methods for qualitative and quantitative analyses of phenolic compounds in fruit peels of P. granatum. For separation of bioactive compounds, the crude ethanolic extracts of P. granatum were fractionated by using column chromatography. These fractions were coded as PG I, PG II, PG III, PG IV and PG V for P. granatum. These fractions were analyzed by thin layer chromatography (TLC) and showed that PG II fraction had an interested compound. The highest total phenolic and flavonoid content were observed in the PG II fraction of P. granatum (617 ± 0.017 mg/g and 546.33 ± 0.032 mg/g respectively) in comparison to other fractions. The phenolic compounds gallic acid and caffeic have been identified in the PG II fraction of P. granatum by using different chromatographic techniques i.e. TLC, HPLC, LC-MS analysis. The overall results of the present studies were indicated that PG II fraction of P. granatum was a good source of phenolic compounds. The present analytical study proved a potential application to identify and quantify the phenolic compounds in the plant fractions. Hence, it could be pursued further for obtaining phytomedicine.

Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens

“…Other than punicalagin, pomegranate peel extracts have been reported to contain pyrogallol, catechin, rutin, cinnamic acid, benzoic acid, chlorogenic acid, acacetin, ferulic acid, kampferol, genistein ( Ali et al, 2014), coumaric acid ( Khan et al, 2017) tannins (punicalin, pedunculagin, GA and ellagic acid) ( Ismail et al, 2012; Pagliarulo et al, 2016), and quercetin ( Saxena et al, 2017). To gain more insight, we docked all these reported compounds against LuxR analogues of C. violaceum and P. aeruginosa , against which our extract was found to act as signal-response inhibitor.…”

Anti-infective potential of hydroalcoholic extract of Punica granatum peel against gram-negative bacterial pathogens

“…This method is known to be capable of fast extraction of phenolics Proestos & Komaitis, (2008), and also suitable for heat-labile phytoconstituents (Gupta et al, 2012). The fact that same extract when prepared using different extraction methods, can vary with respect to its efficacy, has previously also been emphasized by us (Kothari et al, 2012).…”

“…growth/ virulence, etc.) after one-time-exposure to antimicrobial agents, and may last for many hours depending on the concentration of test agent and the susceptibility of the target bacterium (Pfaller et al, 2004;Ramadan et al, 1995;Ramanuj et al, 2012).…”

Potential of peel extracts of Punica granatum and Citrus aurantifolia on alloxan-induced diabetic rats

“…Gallic acid or 3, 4, 5-trihydroxybenzoic acid is a phenolic acid that exerts antioxidant, anticancer, antiviral, and many other biological effects [33,34]. In addition to punicalagin, major phenolic compounds such as gallic acid and ellagic are also found to be present in P. granatum fruit peel [35] which are responsible for reduced oxidative stress, lipid peroxidation, α-glucosidase inhibition and enhanced uptake of glucose [36].. Similarly, the result of the current study was coincided with another author who examined the gallic acid was extracted by the fruit rind of P. granatum which was identified in TLC chromatographic method [37] which can regenerate beta cells and enhance glucose uptake via GLUT4 [38].…”