Identification and quantification of oxo-bile acids in human faeces with liquid chromatography–mass spectrometry: A potent tool for human gut acidic sterolbiome studies

Franco, Placido; Porru, Emanuele; Fiori, Jessica; Gioiello, Antimo; Cerra, Bruno; Roda, Giulia; Caliceti, Cristiana; Simoni, Patrizia; Roda, Aldo

doi:10.1016/j.chroma.2018.11.038

Cited by 30 publications

(44 citation statements)

References 48 publications

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“…This assay resulted in tentative detection of 14 sulfated bile acids, 6 of which were dehydrogenated (possessing either an alkene or ketone; Table 3 and ref. 30). Many of these bile acids are distinguishable only by retention time, consistent with isomers that differ in the position(s) of double bonds, hydroxyl groups, and/or sulfate.…”

Section: Resultsmentioning

confidence: 99%

Metabolomic networks connect host-microbiome processes to human Clostridioides difficile infections

Robinson¹,

Weir²,

Crowley³

et al. 2019

Journal of Clinical Investigation

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Section: Resultsmentioning

confidence: 99%

Metabolomic networks connect host-microbiome processes to human Clostridioides difficile infections

Robinson¹,

Weir²,

Crowley³

et al. 2019

Journal of Clinical Investigation

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“…The majority of BAs in feces were determined by HPLC-MS [5,[139][140][141][142][143][144][145]. Cai et al [141] developed an HPLC-ESI-MS method for the determination of six major BAs in fecal samples.…”

Section: Analysis Of Bile Acids In Fecesmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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“…Deviations from the responses of the two solutions are identified as ion enhancement or suppression. The advantage of this approach over the previous one is that it provides a quantitative and more accurate assessment of MEs [40][41][42]50].…”

Section: The Matuszewski Post-extraction Spike Methodsmentioning

confidence: 99%

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

et al. 2020

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In recent decades, mass spectrometry techniques, particularly when combined with separation methods such as high-performance liquid chromatography, have become increasingly important in pharmaceutical, bio-analytical, environmental, and food science applications because they afford high selectivity and sensitivity. However, mass spectrometry has limitations due to the matrix effects (ME), which can be particularly marked in complex mixes, when the analyte co-elutes together with other molecules, altering analysis results quantitatively. This may be detrimental during method validation, negatively affecting reproducibility, linearity, selectivity, accuracy, and sensitivity. Starting from literature and own experience, this review intends to provide a simple guideline for selecting the best operative conditions to overcome matrix effects in LC-MS techniques, to obtain the best result in the shortest time. The proposed methodology can be of benefit in different sectors, such as pharmaceutical, bio-analytical, environmental, and food sciences. Depending on the required sensitivity, analysts may minimize or compensate for ME. When sensitivity is crucial, analysis must try to minimize ME by adjusting MS parameters, chromatographic conditions, or optimizing clean-up. On the contrary, to compensate for ME analysts should have recourse to calibration approaches depending on the availability of blank matrix. When blank matrices are available, calibration can occur through isotope labeled internal standards and matrix matched calibration standards; conversely, when blank matrices are not available, calibration can be performed through isotope labeled internal standards, background subtraction, or surrogate matrices. In any case, an adjusting of MS parameters, chromatographic conditions, or a clean-up are necessary.

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Identification and quantification of oxo-bile acids in human faeces with liquid chromatography–mass spectrometry: A potent tool for human gut acidic sterolbiome studies

Cited by 30 publications

References 48 publications

Metabolomic networks connect host-microbiome processes to human Clostridioides difficile infections

Metabolomic networks connect host-microbiome processes to human Clostridioides difficile infections

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

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