Identification and quantification of cytotoxic phenolic acids and flavonoids in Ixora brachiata by UHPLC-DAD and UHPLC-ESI-MS/MS

Bhagya, N.; Chandrashekar, K. R.

doi:10.1016/j.ijms.2020.116290

Cited by 18 publications

(5 citation statements)

References 15 publications

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“…For instance, Bhagya et al. designed a ultra‐high performance liquid chromatography (UHPLC) – diode array detector (DAD) coupled with electrospray ionization mass spectroscopy (UHPLC‐ESI‐MS/MS) platform for chromatographic detection of CA , Gómez‐Mejía et al. detected the CA by matrix solid‐phase dispersion extraction assisted by titanium dioxide nanoparticles , Moreno‐González et al.…”

Section: Introductionsupporting

confidence: 83%

“…In the last few years, many techniques have been developed for determination of CA. For instance, Bhagya et al designed a ultra-high performance liquid chromatography (UHPLC) -diode array detector (DAD) coupled with electrospray ionization mass spectroscopy (UHPLC-ESI-MS/MS) platform for chromatographic detection of CA [13], Gómez-Mejía et al detected the CA by matrix solid-phase dispersion extraction assisted by titanium dioxide nanoparticles [14], Moreno-González et al measured the CA by liquid chromatography-mass spectrometry [15], and Pelle et al detected the CA by electrochemical method with synthesized nanocomposite such as tungsten disulfide (WS 2 ) flakes decorated with catechincapped gold nanoparticles [16]. Among them, voltammetric determination of polyphenols including CA has gained the most attention due to its stability, fast response and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Fast, Simple and Sensitive Determination of Coumaric Acid in Fruit Juice Samples by Magnetite Nanoparticles‐zeolitic Imidazolate Framework Material

Şenocak

2020

Electroanalysis

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In this work, magnetic iron nanoparticle (Fe 3 O 4) was functionalized a zeolitic imidalozate framework (ZIF-4) material by co-precipitation process to provide a magnetic hybrid nanomaterial, viz. Fe 3 O 4 @ZIF-4. The structural characterization was performed different spectroscopic, thermal and microscopic techniques. Moreover, voltammetric sensor properties of Fe 3 O 4 @ZIF-4 nanohybrid material for determination of p-coumaric acid (CA) were performed by differential pulse (DPV) and cyclic voltammetry (CV). A reliable and selective electroanalytical method exhibited for the detection of CA. It was found that the deposition of Fe 3 O 4 @ZIF-4 nanohybrid on a glassy carbon electrode (GCE) brought about 2.3, 3.5 and 5.0 fold more sensitive than Fe 3 O 4 /GCE, ZIF-4/GCE and unmodified GCE responses to CA in Britton-Robinson (BÀ R) buffer solution (a pH of 4). The detection, quantification limits and linear range of CA were determined as 0.18, 0.60 μM and 0.50-12.00 μM. The stability, repeatability and selectivity of the suggested Fe 3 O 4 @ZIF-4 modified GCE sensor was fully investigated. In addition, an application of orange juices samples was performed on modified electrode for determination of CA, which was showed by a standard addition method with verifying of High Performance Liquid Chromatography (HPLC) analysis. Besides the ease of synthesis and low price, the suggested Fe 3 O 4 @ZIF-4/GCE sensor offers repeatability, selectivity, stability and easy to apply to real samples.

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Section: Introductionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Fast, Simple and Sensitive Determination of Coumaric Acid in Fruit Juice Samples by Magnetite Nanoparticles‐zeolitic Imidazolate Framework Material

Şenocak

2020

Electroanalysis

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“…In addition, peak 2 , showing a precursor ion of m/z 137.0220 and fragment ions of m/z 119.0122, m/z 108.0208 and m/z 92.0255, was putatively identified as p -hydroxybenzoic acid according to the literature [ 27 ]. Similarly, Peaks 8 was putatively identified as p -coumaric acid [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Chemometrics integrated with in silico pharmacology to reveal antioxidative and anti-inflammatory markers of dandelion for its quality control

et al. 2022

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Background Dandelion is an herb with high nutritional and medicinal values, which has been listed in Chinese Pharmacopeia, European Pharmacopoeia and British Pharmacopoeia, gaining increasing acceptance around the world. However, the current quality control of dandelion is lagging behind. Only in Chinese Pharmacopeia, cichoric acid is used as a marker compound for its quality evaluation, whereas, it can not comprehensively reflect the bioactivity of dandelion. Methods This study developed a strategy by integrating chemometrics with in silico pharmacology to reveal the bioactive markers of dandelion for its quality control. Firstly, the major chemicals in dandelion were characterized using HPLC-DAD-MS/MS, and the corresponding antioxidant and anti-inflammatory activities were evaluated in vitro. Subsequently, the active components were screened by relating the chemicals and bioactivity of dandelion via grey relational assay and partial least squares regression analysis. The potential active components were then subjected to a validation for their activities. Moreover, in silico pharmacology was utilized to evaluate the contribution of active components to efficacy. Results A total of 22 phenolic compounds were characterized. Among them, cichoric acid, caffeic acid and luteolin were identified as quality markers owing to their good correlations with the bioactivities of dandelion. These three markers were quantified in frequently-used dandelion species, viz. Taraxacum mongolicum Hand.-Mazz. (TAM) and T. officinale F. H. Wigg. (TAO). TAM, with acceptably higher content of cichoric acid and caffeic acid, showed better antioxidant activity than TAO. While TAO included higher content of luteolin, presenting slightly more effective in anti-inflammation. Conclusion An useful strategy for the quality marker discovery was successfully designed. And the results provided more knowledge for the quality evaluation of dandelion.

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“…Compound 12 exhibited a parent ion at m / z 319.0453 [M+H] + as well as fragment ions at m / z 245.0468 [(M+H)-C 3 H 6 O 2 ] + , 217.0505 [(M+H)-C 3 H 6 O 2 -CO] + , and 153.0187 [(M+H)-C 7 H 2 O 5 ] + , due to the loss of 74 amu, 102 amu, and 166 amu. According to the similar fragment pattern, this compound was distinctly identified as myricetin [ 31 ]. Similarly, compound 13 , which showed a molecular ion at m / z 303.0504 [M+H] + , with MS 2 fragment ions at 229.0503 [(M+H)-C 3 H 6 O 2 ] + , 153.0817 [(M+H)-C 7 H 2 O 4 ] + , 137.0232 [(M+H)-C 7 H 2 O 5 ] + , was easily identified as quercetin.…”

Section: Resultsmentioning

confidence: 99%

Free, Conjugated, and Bound Phenolics in Peel and Pulp from Four Wampee Varieties: Relationship between Phenolic Composition and Bio-Activities by Multivariate Analysis

et al. 2022

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Free, conjugated, and bound phenolic fractions of peel and pulp in four wampee varieties from South China were analyzed for their contents, composition, antioxidant capacities, and inhibitory activities against α-glucosidase. We found that there were significant differences in phenolic/flavonoid contents among diverse varieties and different parts (peel and pulp), and the contents were highest in the peel’s bound form. The results of UHPL-Q-Exactive HF-X and HPLC showed that chlorogenic acid, gentisic acid, and rutin were abundantly distributed over the three phenolic fractions in peel and pulp of all wampee samples, while isoquercitrin was the most abundant in the conjugated form of peel/pulp and myricetin had the richest content in the free form of peel/pulp. Wampee peel had stronger antioxidant capacities of ABTS+, DPPH, ·OH, and FRAP than the pulp, and the bound phenolic fraction of the peel/pulp had much higher antioxidant activities than FP and CP fractions. It is interesting that the same phenolic fraction of the wampee peel displayed roughly close IC50 values of α-glucosidase inhibition to those from the pulp samples. The relationship between individual phenolic and TPC/TFC/the bio-activities and the similarity among the free, conjugated, and bound phenolic fractions in peel and pulp samples were explored by using Pearson correlation analysis, principal component analysis, and hierarchical cluster analysis. This work provides a systematic and comprehensive comparison of the three phenolic fractions of diverse wampee varieties and different parts, and a rationale for applying phenolics from wampee fruits.

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Identification and quantification of cytotoxic phenolic acids and flavonoids in Ixora brachiata by UHPLC-DAD and UHPLC-ESI-MS/MS

Cited by 18 publications

References 15 publications

Fast, Simple and Sensitive Determination of Coumaric Acid in Fruit Juice Samples by Magnetite Nanoparticles‐zeolitic Imidazolate Framework Material

Fast, Simple and Sensitive Determination of Coumaric Acid in Fruit Juice Samples by Magnetite Nanoparticles‐zeolitic Imidazolate Framework Material

Chemometrics integrated with in silico pharmacology to reveal antioxidative and anti-inflammatory markers of dandelion for its quality control

Free, Conjugated, and Bound Phenolics in Peel and Pulp from Four Wampee Varieties: Relationship between Phenolic Composition and Bio-Activities by Multivariate Analysis

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