Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of <i>Gymnema sylvestre</i> Using HPLC-PDA and LC-MS/MS

Satheeshkumar, N.; Saladi, Shantikumar; Komali, Mopuri

doi:10.1155/2014/518175

Cited by 9 publications

(7 citation statements)

References 10 publications

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“…Previous studies reveal that caffeic acid and kaempferol are the major phytoconstituents of G. sylvestra N. sativa, T. foenum-groecum , and Tinospora cordifolia , which act as the potent inhibitor of α-amylase and α-glucosidase activities that delays the digestion of starch and disaccharides to absorbable monosaccharides, resulting in a reduction of postprandial hyperglycemia. 24–28…”

Section: Resultsmentioning

confidence: 99%

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TLC-bioautography-MS-based Identification of Antioxidant, α-Amylase and α-Glucosidase Inhibitory Compounds in a Polyherbal Formulation “Sugreen-120”

Gaurav

Anwar²,

Zahiruddin

et al. 2023

Pharmacognosy Magazine

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Background Sugreen-120 is one of the famous Indian polyherbal formulations used for the treatment of diabetes. Due to a lack of scientific evidence, the present study is aimed at investigating the phytopharmacology of Sugreen-120 concerning its antioxidant and antidiabetic characteristics. Materials and Methods Total phenols and flavonoid content followed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity was estimated in Sugreen-120. α-Amylase and α-glucosidase inhibitory action of Sugreen-120 was estimated to evaluate its antidiabetic potential. Thin layer chromatography (TLC)-bioautography-MS analysis was performed to determine DPPH free radical, α-amylase and α-glucosidase inhibitory phytoconstituents in Sugreen-120. High performance thin layer chromatography (HPTLC)-based quantitative analysis of Sugreen-120 was performed for the simultaneous separation of caffeic acid and kaempferol. In silico docking analysis was performed to determine the effect of Sugreen-120 metabolites against α-amylase and α-glucosidase enzymes. Results The results showed that Sugreen-120 is enriched in total phenols and flavonoids and even has good potential to scavenge DPPH free radicals with an inhibitory concentration (IC50) value of 414.59 ± 4.925 µg/mL. In α-amylase and α-glucosidase inhibitory assays, the efficacy of Sugreen-120 was found in a dose-dependent manner and the IC50 values were found as 220.106 ± 1.375 and 441.44 ± 1.992 µg/mL, respectively. TLC-bioautographic analysis showed that 06 constituents were found active against DPPH free radical, 04 constituents active against α-amylase and 03 constituents active against α-glucosidase. A HPTLC quantitative study revealed the content of caffeic acid and kaempferol to be 5.233 ± 0.026 and 16.959 ± 0.036 µg/mg, respectively. In silico docking analysis showed that out of 5 identified metabolites, myricetin, ellagic acid, and kaempferol were found with significant interaction with α-amylase and α-glucosidase proteins. Hence, it can be concluded that Sugreen-120 exhibits not only an antidiabetic effect but also antioxidant potential. It can be a palliative choice and an alternative that can be used for the treatment of diabetes.

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Section: Resultsmentioning

confidence: 99%

“…foenum-groecum, and Tinospora cordifolia, which act as the potent inhibitor of α-amylase and α-glucosidase activities that delays the digestion of starch and disaccharides to absorbable monosaccharides, resulting in a reduction of postprandial hyperglycemia. [24][25][26][27][28] In Silico Docking Analysis…”

Section: Quantitative Analysis Of Marker Compounds Inmentioning

confidence: 99%

TLC-bioautography-MS-based Identification of Antioxidant, α-Amylase and α-Glucosidase Inhibitory Compounds in a Polyherbal Formulation “Sugreen-120”

Gaurav

Anwar²,

Zahiruddin

et al. 2023

Pharmacognosy Magazine

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“…Elucidation of the structures of fragments of quercetin was carried out by examination of ESI‐MS/MS spectra of its [M + H] + ion ( m/z 303). The ESI‐MS/MS spectra of quercetin showed abundant ions at m/z 257 (loss of CH 2 O 2 ), m/z 165 (loss of C 7 H 6 O 3 ), m/z 137 (loss of C 8 H 5 O 4 ), and m/z 153 (loss of C 8 H 5 O 3 ) . We chose the m/z 303 and 153 daughter ions for quantitation.…”

Section: Resultsmentioning

confidence: 99%

A Fast and Efficient UPLC‐ESI‐MS/MS Method for Detection, Identification, and Quantitative Analysis of Bioactive Substances in Medicinal Herbal Extracts of Preventing Hair Loss

Lee

Shon

Yoon³

2015

Bulletin Korean Chem Soc

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A rapid, sensitive, and selective method involving ultraperformance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) was developed for the qualitative and quantitative analysis of the constituents in a medicinal herbal complex extract (MHCE). The MHCE was obtained from 23 herbs traditionally used in oriental medicines that we have been developed as new therapeutic materials to prevent hair loss and enhance hair growth. The chromatographic analysis of the samples of MHCE was performed on a reversed-phase C 18 (10 cm × 2.1 mm, flow rate 0.3 mL/min) column with gradient elution using acetonitrile and water with 0.1% formic acid. Mass spectrometry was performed in the positive ESI mode. The bioactive compounds were detected by selected reaction monitoring (SRM) with UPLC-ESI-MS/MS. All calibration curves of reference compounds showed good linearity (r 2 > 0.9964) in the test ranges. The limit of detection and limit of quantification of the method were 0.02 ng/mL and 0.05 ng/mL, respectively.

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“…The complexity of chemicals in Traditional Chinese Medicine (TCM) including P. freyniana has presented a significant challenge in the rapid identification and characterization of components. During the past decades, HPLC-MS, as a new technique has been used to profile and identify the chemical in TCM due to its validity, sensitivity, and specialness [ 10 , 11 ]. Especially, UHPLC-HRMS such as UHPLC-Q-Exactive Orbitrap MS, UHPLC-Q-TOF MS, and UHPLC–LTQ-Orbitrap-MS, affording a higher and faster separation and higher resolution of mass, was a much more powerful equipment in the identification of TCM compared to traditional HPLC-MS [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification and Systematic Mechanism of Flavonoids from Potentilla freyniana Bornm. Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Network Pharmacology

Cai

Qin

et al. 2021

International Journal of Analytical Chemistry

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Potentilla freyniana Bornm. (P. freyniana), belonging to the family Rosaceae, has been used as a folk medicine in China. However, as we know, the constituents and the systematic elucidation of the mechanism were not fully investigated. Therefore, it is necessary to develop a rapid method using LC-MS and network pharmacology for the detection and identification of constituents and the systematic mechanism of P. freyniana. Firstly, the flavonoids were detected and identified based on ultra-high-performance liquid chromatography coupled with Quadrupole-Exactive Focus Orbitrap MS (UHPLC-Q-Exactive Orbitrap MS). After that, the potential targets of those constituents were obtained by database mining. Then, the core targets were predicted by protein-protein interaction network and network analysis. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out via DAVID. This finding revealed that P. freyniana possessed 43 flavonoids (40 of them were first reported) with 23 core target genes, which are associated with PI3K-Akt, MAPK, TNF signaling pathway, and pathway in cancer. This study demonstrated the multicompound, multitarget, and multimechanism of P. freyniana, which are very beneficial to develop the further study and utilization of this plant including the material basis and quality control research.

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Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS/MS

Cited by 9 publications

References 10 publications

TLC-bioautography-MS-based Identification of Antioxidant, α-Amylase and α-Glucosidase Inhibitory Compounds in a Polyherbal Formulation “Sugreen-120”

TLC-bioautography-MS-based Identification of Antioxidant, α-Amylase and α-Glucosidase Inhibitory Compounds in a Polyherbal Formulation “Sugreen-120”

A Fast and Efficient UPLC‐ESI‐MS/MS Method for Detection, Identification, and Quantitative Analysis of Bioactive Substances in Medicinal Herbal Extracts of Preventing Hair Loss

Rapid Identification and Systematic Mechanism of Flavonoids from Potentilla freyniana Bornm. Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Network Pharmacology

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