Identification and profiling of microRNAs in two developmental stages of the model cestode parasite Mesocestoides corti

Basika, Tatiana; Macchiaroli, Natalia; Cucher, Marcela Alejandra; Espinola, Sergio; Kamenetzky, Laura; Zaha, Arnaldo; Rosenzvit, Mara Cecília; Ferreira, Henrique Bunselmeyer

doi:10.1016/j.molbiopara.2016.08.004

Cited by 30 publications

(38 citation statements)

References 64 publications

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“…The stage specific novel miR like miR-12071 was found in tetrathyridia stage and three conserved miRs found in storbilated worms were miR-2b, miR-7a, and miR-3479b. Besides these novel miRs there were certain common miRs and are highly expressed in both stages like bantam-3p, let-7-5p, miR-10-5p, miR-71-5p, and miR-4989-3p (Basika et al, 2016). The significance of miR-71 in Echinococcus spp.…”

Section: Mir Host Immune Modulation In Cestodementioning

confidence: 99%

“…But whether the same mechanism occurs in M. corti needs further investigation. The up-regulated miR-36b also found in Echinococcus is associated with regenerative potential in planarians (Friedländer et al, 2009) and rapid proliferation in Echinococcus (Cucher et al, 2015) in M. corti tetrahyridia stage this miR is thought to control proliferation by asexual reproduction (Basika et al, 2016). The miR profile showed similarly expressed miRs in Echinococcus.…”

Section: Mir Host Immune Modulation In Cestodementioning

confidence: 99%

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Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

et al. 2017

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Helminthic infections fall under neglected tropical diseases, although they inflict severe morbidity to human and causes major economic burden on health care system in many developing countries. There is increased effort to understand their immunopathology in recent days due to their immuno-modulatory capabilities. Immune response is primarily controlled at the transcriptional level, however, microRNA-mediated RNA interference is emerging as important regulatory machinery that works at the translation level. In the past decade, microRNA (miRNA/miR) research has advanced with significant momentum. The result is ever increasing list of curated sequences from a broad panel of organisms including helminths. Several miRNAs had been discovered from trematodes, nematodes and cestodes like let-7, miR155, miR-199, miR-134, miR-223, miR-146, and fhe-mir-125a etc., with potential role in immune modulation. These miRs had been associated with TGF-β, MAPK, Toll-like receptor, PI3K/AKT signaling pathways and insulin growth factor regulation. Thus, controlling the immune cells development, survival, proliferation and death. Apart from micromanagement of immune system, they also express certain unique miRNA also like cis-miR-001, cis-miR-2, cis-miR-6, cis-miR-10, cis-miR-18, cis-miR-19, trs-mir-0001, fhe-miR-01, fhe-miR-07, fhe-miR-08, egr-miR-4988, egr-miR-4989 etc. The specific role played by most of these species specific unique miRs are yet to be discovered. However, these newly discovered miRNAs might serve as novel targets for therapeutic intervention or biomarkers for parasitic infections.

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Section: Mir Host Immune Modulation In Cestodementioning

confidence: 99%

Section: Mir Host Immune Modulation In Cestodementioning

confidence: 99%

Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

et al. 2017

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“…For determination of vitality, three groups of 10 cysticerci each (stages I and II) from the peritoneal cavity of the same mouse of each replicate were maintained in parallel in DMEM and in DMEM+DMSO (100 mM (Cucher et al, 2015;Macchiaroli et al, 2015) and M. corti (Basika et al, 2016) as reported by our research group. All the annotated sequences, together with the novel miRNA precursor sequences identified in this study, were used to construct an in-house database for small RNA library data classification available at http://www.bmhid.org/downloads/.…”

Section: Experimental Design and Parasite Culturementioning

confidence: 99%

“…In addition, miRNA-SOM allowed us to confirm two miRNA precursors, pre-mir-new-1a and pre-mir-new-1b, that were only described before in the E. granulosus s. s. G1 genotype (Bai et al, 2014). Since a dominant mature miRNA can be processed either from the 5' or 3' arm of the corresponding pre-miRNA, we considered that both arms of the same hairpin produced two mature miRNAs when the number of read counts of the minor product represented ≥ 30% of the read counts from the major product originating from the opposite arm (Cucher et al, 2015;Basika et al, 2016). By doing this, we observed that pre-mir-31 and pre-mir-new-1a showed expression from both arms under both conditions ( Supplementary Tables S2 and S3).…”

Section: Taenia Crassiceps and T Solium Mirna Repertoirementioning

confidence: 99%

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microRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs

Pérez

Macchiaroli

Lichtenstein

et al. 2017

International Journal for Parasitology

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MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T. solium genome. Moreover, we analysed the effect of praziquantel, one of the two main drugs used for cysticercosis treatment, on the miRNA expression profile of T. crassiceps cysticerci. Using small RNA-seq and two independent algorithms for miRNA prediction, as well as northern blot validation, we found transcriptional evidence of 39 miRNA loci in T. crassiceps. Since miRNAs were mapped to the T. solium genome, these miRNAs are considered common to both parasites. The miRNA expression profile of T. crassiceps was biased to the same set of highly expressed miRNAs reported in other cestodes. We found a significant altered expression of miR-7b under praziquantel treatment. In addition, we searched for miRNAs predicted to target genes related to drug response. We performed a detailed target prediction for miR-7b and found genes related to drug action. We report an initial approach to study the effect of sub-lethal drug treatment on miRNA expression in a cestode parasite, which provides a platform for further studies of miRNA involvement in drug effects. The results of our work could be applied to drug development and provide basic knowledge of cysticercosis and other neglected helminth infections.

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Comparison of different staining methods for determination of viability on Mesocestoides vogae tetrathyridia

Fabbri

Elissondo

2018

Parasitol Res

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Identification and profiling of microRNAs in two developmental stages of the model cestode parasite Mesocestoides corti

Cited by 30 publications

References 64 publications

Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

Micromanagement of Immune System: Role of miRNAs in Helminthic Infections

microRNA analysis of Taenia crassiceps cysticerci under praziquantel treatment and genome-wide identification of Taenia solium miRNAs

Comparison of different staining methods for determination of viability on Mesocestoides vogae tetrathyridia

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