Identification and profiling of Bactrocera dorsalis microRNAs and their potential roles in regulating the developmental transitions of egg hatching, molting, pupation and adult eclosion

Zhang, Qiang; Dou, Wei; Song, Zhong-Hao; Jin, Tong-Jun; Yuan, Guangjie; Schutter, Kristof De; Smagghe, Guy; Wang, Jinjun

doi:10.1016/j.ibmb.2020.103475

Cited by 25 publications

(20 citation statements)

References 49 publications

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“…In insects, modulating the abundance of let-7 altered the tolerance of Myzus persicae nicotianae to nicotine ( Peng et al, 2016 ). Let-7 was also required in many developmental processes, such as sleep homeostasis ( Goodwin et al, 2018 ), age-dependent behavioral changes ( Behura and Whitfield, 2010 ), developmental transitions of egg hatching, molting, pupation, adult eclosion ( Zhang et al, 2020 ) and the regulation of ecdysone levels ( Liu et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

“…MiR-279, miR-306, and miR-308 were also predicted to be involved in the Destruxin A-responsive immunity process in DBM ( Shakeel et al, 2018 ), indicating that these miRNA families might be closely associated with Bt resistance. Novel-miR-21 was classified into the miR-317 family, and miR-317 was found to be involved in the process of pupation in B. dorsalis ( Zhang et al, 2020 ). In Drosophila , miR-87 was an important regulator of dendrite regeneration ( Kitatani et al, 2020 ), so we speculated that novel-miR-199 as a member of the miR-87 family, might paly the similar roles in DBM.…”

Section: Discussionmentioning

confidence: 99%

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Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance

Yang

Lin

et al. 2021

Front. Genet.

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The diamondback moth (DBM), Plutella xylostella, one of the most destructive lepidopteran pests worldwide, has developed field resistance to Bacillus thuringiensis (Bt) Cry toxins. Although miRNAs have been reported to be involved in insect resistance to multiple insecticides, our understanding of their roles in mediating Bt resistance is limited. In this study, we constructed small RNA libraries from midguts of the Cry1Ac-resistant (Cry1S1000) strain and the Cry1Ac-susceptible strain (G88) using a high-throughput sequencing analysis. A total of 437 (76 known and 361 novel miRNAs) were identified, among which 178 miRNAs were classified into 91 miRNA families. Transcripts per million analysis revealed 12 differentially expressed miRNAs between the Cry1S1000 and G88 strains. Specifically, nine miRNAs were down-regulated and three up-regulated in the Cry1S1000 strain compared to the G88 strain. Next, we predicted the potential target genes of these differentially expressed miRNAs and carried out GO and KEGG pathway analyses. We found that the cellular process, metabolism process, membrane and the catalytic activity were the most enriched GO terms and the Hippo, MAPK signaling pathway might be involved in Bt resistance of DBM. In addition, the expression patterns of these miRNAs and their target genes were determined by RT-qPCR, showing that partial miRNAs negatively while others positively correlate with their corresponding target genes. Subsequently, novel-miR-240, one of the differentially expressed miRNAs with inverse correlation with its target genes, was confirmed to interact with Px017590 and Px007885 using dual luciferase reporter assays. Our study highlights the characteristics of differentially expressed miRNAs in midguts of the Cry1S1000 and G88 strains, paving the way for further investigation of miRNA roles in mediating Bt resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance

Yang

Lin

et al. 2021

Front. Genet.

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“…The first miRNA, lin‐14, was discovered in the Caenorhabditis elegans , and the first insect miRNA has been identified in the Drosophila melanogaster back in 2001 (Lagos‐Quintana et al, 2001; Lee et al, 1993; Wightman & Ruvkun, 1993). For the past few years, the rapid development of high‐throughput sequencing technologies has enabled the efficient recognition of miRNAs and the study of their functions in the regulation of vital biological and physiological processes (Q. Zhang, Dou, et al, 2021). Recently, a mass of miRNAs has been identified in many insect pests.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a mass of miRNAs has been identified in many insect pests. In insects, a total of 1154 miRNAs have been identified in Tribolium castaneum , 462 in P. xylostella , 407 in B. mori , 265 in Sitobion miscanthi , 250 in Bactrocera dorsalis , 220 in Nilaparvata lugens , 186 in Helicoverpa armigera , 176 in D. melanogaster , 169 in Periplaneta americana , 127 in S. exigua , and 79 in Laodelphax striatellus (Fullaondo & Lee, 2012; J. M. Li et al, 2014; Liang et al, 2013; Lomate et al, 2014; Mao et al, 2021; Wu et al, 2017; Yang et al, 2020; H. Yin, Zhang, et al, 2021; Y. L. Zhang et al, 2015; Q. Zhang et al, 2020; B. Z. Zhang, Hu, et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Systematic identification and characterization of differentially expressed microRNAs under tetraniliprole exposure in the fall armyworm, Spodoptera frugiperda

Zhang

Wang

Yu³

et al. 2022

Arch Insect Biochem Physiol

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The fall armyworm, Spodoptera frugiperda, is a worldwide agricultural pest and causes huge losses of crop production each year. Tetraniliprole is a novel diamide insecticide with high efficacy against even the insecticide resistant pests of Lepidoptera, Coleoptera, and Diptera. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level and play an important regulatory role in the insecticide resistance in insects. However, the effects of miRNAs on the tetraniliprole tolerance in S. frugiperda are poorly understood. In the present research, the miRNAs response to tetraniliprole application in S. frugiperda were systematically investigated by high‐throughput sequencing. A total of thirty differentially expressed miRNAs were identified under tetraniliprole treatment in S. frugiperda. The functions of the target genes of these differentially expressed miRNAs were further predicted by Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes database pathway, and the most significantly enriched pathway was MAPK signaling pathway. The expression changes of six differentially expressed miRNAs were validated by quantitative real‐time polymerase chain reaction. Furthermore, miR‐278‐5p had the highest expression in the hemolymph and malpighian tubule and the lowest expression in the gut. Oversupply of miR‐278‐5p significantly increased the mortality of S. frugiperda following exposure to tetraniliprole. These results will provide the basis for understanding the regulatory roles of miRNAs regarding to tetraniliprole tolerance in S. frugiperda.

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“…Previous studies reported that there were striking differences in the brain or head miRNAs expression between nurse and forager [ 21 , 22 , 41 , 42 ]. Hence, development stages have obvious impacts on the expression levels of miRNAs in honeybees [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Age and Behavior-Dependent Differential miRNAs Expression in the Hypopharyngeal Glands of Honeybees (Apis mellifera L.)

Shi

Zhu

Liu

et al. 2021

Insects

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This study aims to investigate the expression differences of miRNAs in the hypopharyngeal glands (HPGs) of honeybees at three developmental stages and to explore their regulation functions in the HPGs development. Small RNA sequencing was employed to analyze the miRNA profiles of HPGs in newly-emerged bees (NEB), nurse bees (NB), and forager bees (FB). Results showed that a total of 153 known miRNAs were found in the three stages, and ame-miR-276-3p, ame-miR-375-3p, ame-miR-14-3p, ame-miR-275-3p, and ame-miR-3477-5p were the top five most abundant ones. Furthermore, the expression of 11 miRNAs, 17 miRNAs, and 18 miRNAs were significantly different in NB vs. FB comparison, NB vs. NEB comparison, and in FB vs. NEB comparison, respectively, of which ame-miR-184-3p and ame-miR-252a-5p were downregulated in NB compared with that in both the FB and NEB, while ame-miR-11-3p, ame-miR-281-3p, and ame-miR-31a-5p had lower expression levels in FB compared with that in both the NB and NEB. Bioinformatic analysis showed that the potential target genes of the differentially expressed miRNAs (DEMs) were mainly enriched in several key signaling pathways, including mTOR signaling pathway, MAPK signaling pathway-fly, FoxO signaling pathway, Hippo signaling pathway-fly. Overall, our study characterized the miRNA profiles in the HPGs of honeybees at three different developmental stages and provided a basis for further study of the roles of miRNAs in HPGs development.

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Identification and profiling of Bactrocera dorsalis microRNAs and their potential roles in regulating the developmental transitions of egg hatching, molting, pupation and adult eclosion

Cited by 25 publications

References 49 publications

Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance

Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance

Systematic identification and characterization of differentially expressed microRNAs under tetraniliprole exposure in the fall armyworm, Spodoptera frugiperda

Age and Behavior-Dependent Differential miRNAs Expression in the Hypopharyngeal Glands of Honeybees (Apis mellifera L.)

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