Age and Behavior-Dependent Differential miRNAs Expression in the Hypopharyngeal Glands of Honeybees (Apis mellifera L.)

Shi, Tengfei; Zhu, Yujie; Liu, Peng; Liang, Ye; Jiang, Xingchuan; Cao, Haiqun; Yu, Linsheng

doi:10.3390/insects12090764

Cited by 7 publications

(3 citation statements)

References 61 publications

(65 reference statements)

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“…The significantly upregulated miRNAs of D. farinae exosomes identified by RNA-Seq analysis were also highly expressed in other arthropods ( 29 , 35 ). As the most abundant miRNA, miR-276 was reported to promote developmental synchrony in Locusta migratoria ( 36 ) and to shape the mosquito reproductive cycle and Plasmodium falciparum development through modulating metabolic balance ( 37 ).…”

Section: Discussionmentioning

confidence: 88%

Exosomes Derived from Dermatophagoides farinae Induce Allergic Airway Inflammation

Yang

et al. 2023

Microbiol Spectr

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Dermatophagoides farinae , a predominant species of house dust mites in China, has displayed pathogenic role in allergic disorders, and exosomes derived from human bronchoalveolar lavage fluid have been strongly associated with allergic respiratory diseases progression. However, the pathogenic role of D. farinae -derived exosomes in allergic airway inflammation has remained unclear until now.

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Section: Discussionmentioning

confidence: 88%

Exosomes Derived from Dermatophagoides farinae Induce Allergic Airway Inflammation

Yang

et al. 2023

Microbiol Spectr

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“…MiRNAs are endogenous small RNAs (sRNAs) ranging from 18 nt to 25 nt in length [ 4 ]. Accumulating evidence demonstrates that miRNAs are not only crucial regulators in cell metabolism, proliferation, differentiation, and apoptosis [ 5 , 6 , 7 ], but also key players in modulating a great number of pivotal biological processes such as development and immunity [ 8 , 9 ]. In honey bees, miRNAs have been suggested to be involved in the regulation of development [ 10 ], queen spawning [ 11 ], dance behavior [ 12 ], and immune defense [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

ame-miR-34 Modulates the Larval Body Weight and Immune Response of Apis mellifera Workers to Ascosphara apis Invasion

Guo

Fan

et al. 2023

IJMS

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MiRNAs are critical regulators of numerous physiological and pathological processes. Ascosphaera apis exclusively infects bee larvae and causes chalkbrood disease. However, the function and mechanism of miRNAs in the bee larval response to A. apis infection is poorly understood. Here, ame-miR-34, a previously predicted miRNA involved in the response of Apis mellifera larvae to A. apis invasion, was subjected to molecular validation, and overexpression and knockdown were then conducted to explore the regulatory functions of ame-miR-34 in larval body weight and immune response. Stem-loop RT-PCR and Sanger sequencing confirmed the authenticity of ame-miR-34 in the larval gut of A. mellifera. RT-qPCR results demonstrated that compared with that in the uninfected larval guts, the expression level of ame-miR-34 was significantly downregulated (p < 0.001) in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae, indicative of the remarkable suppression of host ame-miR-34 due to A. apis infection. In comparison with the corresponding negative control (NC) groups, the expression level of ame-miR-34 in the larval guts in the mimic-miR-34 group was significantly upregulated (p < 0.001), while that in the inhibitor-miR-34 group was significantly downregulated (p < 0.01). Similarly, effective overexpression and knockdown of ame-miR-34 were achieved. In addition, the body weights of 5- and 6-day-old larvae were significantly increased compared with those in the mimic-NC group; the weights of 5-day-old larvae in the inhibitor-miR-34 group were significantly decreased in comparison with those in the inhibitor-NC group, while the weights of 4- and 6-day-old larvae in the inhibitor-miR-34 group were significantly increased, indicating the involvement of ame-miR-34 in modulating larval body weight. Furthermore, the expression levels of both hsp and abct in the guts of A. apis-infected 4-, 5-, and 6-day-old larvae were significantly upregulated after ame-miR-34 overexpression. In contrast, after ame-miR-34 knockdown, the expression levels of the aforementioned two key genes in the A. apis-infected 4-, 5-, and 6-day-old larval guts were significantly downregulated. Together, the results demonstrated that effective overexpression and knockdown of ame-miR-34 in both noninfected and A. apis-infected A. mellifera larval guts could be achieved by the feeding method, and ame-miR-34 exerted a regulatory function in the host immune response to A. apis invasion through positive regulation of the expression of hsp and abct. Our findings not only provide a valuable reference for the functional investigation of bee larval miRNAs but also reveal the regulatory role of ame-miR-34 in A. mellifera larval weight and immune response. Additionally, the results of this study may provide a promising molecular target for the treatment of chalkbrood disease.

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“…In honey bees, miRNAs play a vital role in maze-based visual pattern learning and memory [27]. In forager bees, miR-11-3p and miR-281-3p are responsible for honey processing [28].…”

Section: Introductionmentioning

confidence: 99%

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

Huang

Wang

Qiu

et al. 2023

Genes

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MicroRNAs (miRNAs) play a vital role in the nerve regulation of honey bees (Apis mellifera). This study aims to investigate the differences in expression of miRNAs in a honey bee’s brain for olfactory learning tasks and to explore their potential role in a honey bee’s olfactory learning and memory. In this study, 12 day old honey bees with strong and weak olfactory performances were utilized to investigate the influence of miRNAs on olfactory learning behavior. The honey bee brains were dissected, and a small RNA-seq technique was used for high-throughput sequencing. The data analysis of the miRNA sequences revealed that 14 differentially expressed miRNAs (DEmiRNAs) between the two groups, strong (S) and weak (W), for olfactory performance in honey bees were identified, which included seven up-regulated and seven down-regulated. The qPCR verification results of the 14 miRNAs showed that four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory learning and memory. The target genes of these DEmiRNAs were subjected to the GO database annotation and KEGG pathway enrichment analyses. The functional annotation and pathway analysis showed that the neuroactive ligand-receptor interaction pathway, oxidative phosphorylation, biosynthesis of amino acids, pentose phosphate pathway, carbon metabolism, and terpenoid backbone biosynthesis may be a great important pathway related to olfactory learning and memory in honey bees. Our findings together further explained the relationship between olfactory performance and the brain function of honey bees at the molecular level and provides a basis for further study on miRNAs related to olfactory learning and memory in honey bees.

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Age and Behavior-Dependent Differential miRNAs Expression in the Hypopharyngeal Glands of Honeybees (Apis mellifera L.)

Cited by 7 publications

References 61 publications

Exosomes Derived from Dermatophagoides farinae Induce Allergic Airway Inflammation

Exosomes Derived from Dermatophagoides farinae Induce Allergic Airway Inflammation

ame-miR-34 Modulates the Larval Body Weight and Immune Response of Apis mellifera Workers to Ascosphara apis Invasion

Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)

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