Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons

Futagawa-Saito, Keiko; Suzuki, Masahiro; Ohsawa, Motoyasu; Ohshima, Seinosuke; Sakurai, Naomi; Ba-Thein, William; Fukuyasu, Tsuguaki

doi:10.1111/j.1365-2672.2004.02264.x

Cited by 42 publications

(36 citation statements)

References 33 publications

(30 reference statements)

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“…The discriminating power of sodA or hsp60 sequencing is superior to phenotypic characterization or 16S rRNA sequencing for species identification of staphylococci (24,25,38). Although S. intermedius has been reported to be prevalent among dogs, cats, horses, goats, and pigeons (7,13), these S. intermedius strains with different biotypes might have included S. delphini and S. pseudintermedius.…”

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confidence: 99%

Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

et al. 2007

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Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

et al. 2007

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“…Recently, 18 types of SEs in S. aureus have been reported comprising the five classical (SEA through SEE) and the 13 newly described (SEG through SER and SEU). Also, the existence of the 2 new types (SEC -canine and SE -int ) in S. intermedius has been reported [11,12]. Further work is needed to confirm the existence of these new SE genes in order to clarify the roles of SEs in staphylococcal skin infections in dogs.…”

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Characteristics of Staphylococcus intermedius Isolates from Diseased and Healthy Dogs

Sasaki

Shimizu

Kawano

et al. 2005

The Journal of Veterinary Medical Science

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ABSTRACT. Staphylococcus intermedius isolates from diseased and healthy dogs were examined for production of extracellular enzymes and toxins, and phage patterns. There were no significant differences between the two groups of isolates in the production rates of DNase, protease, lipase, gelatinase, hyaluronidase, hemolysins, protein A, and TSST-1, or in phage patterns. But the production rate of enterotoxins in isolates from diseased dogs was significantly higher than that in isolates from healthy dogs. PFGE analysis was performed with isolates from different body sites in individual dogs. In 3 of 6 healthy dogs, identical PFGE patterns were seen in isolates from the nares, external auditory meatus or skin. The remaining 3 dogs yielded isolates of different patterns. In 4 of 6 diseased dogs, identical patterns were seen in isolates from lesions as well as from the other normal sites. Staphylococcus intermedius has been considered a primary pathogen of skin infections in dogs, such as otitis externa [5,7] and pyoderma [1,22,23]. Some strains of S. intermedius produce a variety of extracellular enzymes and toxins and virulence-associated factors [1,13], which are thought to contribute to the pathogenicity of the organism.S. intermedius is also present in the nasal vestibulum, external auditory canal, anal mucosa, and on the skin surface and hair coat of healthy dogs. Knowledge of the characteristics of the normal S. intermedius flora on these sites in healthy dogs is an important factor in understanding the epidemiology of staphylococcal skin infections in dogs. There are several reports on the population sizes and frequency of the organism at these carrier sites in healthy dogs [2,3,9,22]. Nevertheless, comparison of the characteristics of isolates from different sites in individual healthy dogs has not been studied sufficiently [4]. The organism has frequently been cultured from other body sites besides lesion sites in diseased dogs, but information on the clonal relationship between the organisms in the lesions and those in the nose or skin is lacking.The purpose of this study was to compare characteristics of S. intermedius isolates from diseased dogs with those from healthy dogs by using biological properties and phage typing. In addition, the present study was to characterize isolates from different sites in individual healthy and diseased dogs, by means of pulsed-field gel electrophoresis (PFGE).A total of 96 S. intermedius isolates were subjected to biological characterization and phage typing. Forty-seven isolates were from dogs affected with otitis externa (n=8), dermatitis (n=11), pyoderma (n=7), impedigo (n=4), eczema (n=8), and folliculitis (n=9). Forty-nine isolates were from the mouth (n=3), nares (n=17), external auditory meatus (n=18), and skin (n=11) of apparently healthy dogs. Ninety of the 96 isolates had been used in a previous antimicrobial susceptibility study [26].Isolation of S. intermedius from different sites in individual healthy and diseased dogs was performed. Four of 6 healthy dogs e...

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“…It is a member of the normal flora of dogs and also a major opportunistic pathogen responsible for Correspondence to: D. Chrobak, e-mail: dorota.chrobak@wp.pl common canine skin infections. S. intermedius has also been found in a wide range of other animals and can occasionally cause severe infections in humans (Mahoudeau et al 1997, Tanner et al 2000, Bes et al 2002, Futagawa-Saito et al 2004. During the past few years, there has been confusion about the identifica-tion of this species.…”

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confidence: 99%

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

Chrobak¹,

Kizerwetter-Świda²,

Rzewuska³

et al. 2011

Polish Journal of Veterinary Sciences

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A total of 221 SIG strains were isolated from clinical samples of canine origin submitted to the Diagnostic Laboratory of the Division of Bacteriology and Molecular Biology at the Warsaw University of Life Sciences in Warsaw during the period 2006-2010. The aim of the study was to investigate the frequency of prevalence of methicillin-resistant SIG strains and to determine the MIC values of cephalotin, amoxicillin/clavulanic acid, ciprofloxacin, clindamycin, gentamicin, chloramphenicol, mupirocin for a collection of randomly selected 79 strains belonging to Staphylococcus intermedius group (SIG), including 23 mecA-positive and 56 mecA-negative strains. All isolates were identified as belonging to SIG based on their phenotypic properties and PCR amplification of S. intermedius-specific fragment of the 16S rRNA gene. The mecA gene was detected in 26 (12%) of 221 SIG strains. All tested mecA-negative SIG strains were susceptible to amoxicillin/clavulanic acid and cephalotin. One of the 56 mecA-negative SIG strains was resistant to ciprofloxacin, six (11%) to gentamicin. It was found that sixteen (29%) of 56 mecA-negative SIG strains were resistant to clindamycin. Most of the mecA-positive SIG strains were resistant to ciprofloxacin (96%), clindamycin (96%), and gentamicin (96%). Only one MRSIG strain was resistant to chloramphenicol. All examined mecA-positive SIG strains were found to be susceptible to mupirocin. Our results imply that staphylococcal multidrug resistance has become more prevalent, which could lead to difficulties in effective treatment. With some resistant strains the only therapeutic possibility are antimicrobial agents important in human medicine. New regulations for veterinary medicine concerning appropriate therapy of infections caused by multidrug-resistat staphylococci are needed.

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Identification and prevalence of an enterotoxin-related gene, se-int, in Staphylococcus intermedius isolates from dogs and pigeons

Cited by 42 publications

References 33 publications

Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

Methicillin-ResistantStaphylococcus pseudintermediusin a Veterinary Teaching Hospital

Characteristics of Staphylococcus intermedius Isolates from Diseased and Healthy Dogs

Antibiotic resistance of canine Staphylococcus intermedius group (SIG) - practical implications

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