Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India

Bora, Durlav Prasad; Barman, Nagendra Nath; Das, S. K.; Bhanuprakash, V.; Yogisharadhya, Revanaiah; Venkatesan, Gnanavel; Kumar, Amit; Rajbongshi, Gitika; Khatoon, Elina; Chakraborty, Apurba; Bujarbaruah, K. M.

doi:10.1007/s11262-012-0740-y

Cited by 39 publications

(41 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, a percent identity of 83.8-83.9 and 83.4 with BPSV isolates whereas, 93.6-95.1 and 94.7-95 with PCPV was observed, at nt and aa levels, respectively. It shares a significant identity of 97.8-98.2 and 97.4-98.4 % with ORFV isolates worldwide for which the complete genome has been sequenced and characterized (IA82, SA00, NZ2 and D1701 isolates) as observed in previous reports [1,18]. Among Asian isolates, ORFV JS/04 of China shared maximum percent identity of 99.4 and 99.2 with this isolate under the study.…”

supporting

confidence: 60%

“…However, the presence of viral nucleic acid in skin tissue was identified by molecular diagnostics namely diagnostic PCR and B2L gene cloning/ sequencing. Multiple sequence alignment and phylogenetic tree based on complete B2L sequence have been performed to study the evolutionary relationship with other circulating virus strains/isolates in the country [1,4,18].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Occurrence and identification of contagious ecthyma in blackbuck

et al. 2016

Self Cite

View full text Add to dashboard Cite

A carcass of male free ranging adult blackbuck (Antilope cervicapra) was presented for necropsy examination exhibiting thick confluent nodular skin lesions around the mouth and the dry scaly crusts/fissures on the skin of abdomen, thigh and shoulder with subcutaneous haemorrhages. The skin sample around mouth was found positive for orf virus (ORFV) identified by counterimmunoelectrophoresis and PCR. Histopathology of the mouth skin revealed the hyperkeratinization, epidermal sloughing and epithelial hyperplasia showing acanthosis with rete ridges and few eosinophilic intracytoplasmic inclusion bodies in keratinocytes. Further, comparative B2L gene sequence analysis revealed that the virus isolate from blackbuck had shown 97.8-99.6 and 97.6-99.5 % identity at nucleotide and amino acid levels respectively with Indian isolates and maximum identity with ORFV 79/04, an isolate from India. Phylogenetic analysis based on B2L gene also revealed the same evolutionary relationship that it is closely related to Indian isolates. This seems to be the first report of orf in blackbuck from Indian subcontinent.Keywords Blackbuck Á Mange Á Contagious ecthyma Á B2L gene Á Phylogeny Contagious ecthyma (CE) also known as sore mouth or scabby mouth or orf is a highly contagious, zoonotic viral disease caused by orf virus (ORFV) of the genus parapox virus of the family Poxviridae [3,7]. It affects sheep, goats and other domesticated/wild ruminants namely rein deer, musk ox, mule deer, white-tailed deer, pronghorn fawns and wapiti calves in natural captive/free ranging conditions as well as experimental infection [2,9,16]. It has also been reported in camelids, squirrels and seals [4]. Secondary bacterial and fungal infections are common [2] and sometimes associated with other viral infections namely PPRV [13] and goat pox [17]. Increasing number of reports of CE in sheep and goats has been noticed worldwide and it has been reported in all parts of India and endemic in nature [4,10]. This endemic disease incurs economic losses by causing severe morbidity in adults and mortality in young ones affecting farming community in India [10]. Secondary bacterial infection of orf lesions involving Staphylococcus aureus and Arcanobacterium pyogenes and severity aggravated by associated myiasis have been reported during orf cases [5]. There have also been reports of lambs with dual orf/papilloma virus infection and orf/ sheep pox virus infection [19,20]. Enhanced severity of orf lesions and other complications such as mastitis in ewes and foot rot have been noticed in secondary bacterial and associated fungal infections [10]. Outbreaks of CE in wild animal species from captive or free ranging or a zoological collection could be of considerable significance in virus perpetuation or spill over to nearby domestic small ruminants. Furthermore, the role of wildlife in the epizootiology of orf has not been fully elucidated. In India, neither ORFV sero-prevalence/infections have been reported often nor were systematic attempts on virus antigen ...

show abstract

supporting

confidence: 60%

mentioning

confidence: 99%

Occurrence and identification of contagious ecthyma in blackbuck

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…The disease is zoonotic and globally prevalent. Orf is enzootic in Indian sheep and goats (Hosamani et al, 2007;Dey and Kundu, 2009;Venkatesan et al, 2011;Yogisharadhya et al, 2012;Bora et al, 2012). Orf is characterized by proliferative and often self-limiting skin lesions including papules, vesicles and rapidly growing scabs on the skin of lips, oral mucosa and around the nostrils.…”

Section: Introductionmentioning

confidence: 99%

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Bora¹,

Bhanuprakash²,

Venkatesan³

et al. 2015

Asian J. of Animal and Veterinary Advances

Self Cite

View full text Add to dashboard Cite

The study describes the effect of stabilization on the stability of an indigenous live attenuated orf vaccine with stabilizers like LS, LHT and TAA after lyophilization and also reconstitution with NSS, PBS, distilled water and 1 M MgSO 4 exposed at different temperatures under established lyophilization conditions. The stability of the vaccine was assessed both in freeze-dried and reconstituted forms at different temperatures. The results indicated that the orf vaccine lyophilized with LS stabilizer at 25 and 45°C and LHT at 37°C found superior over others in stabilizing the keeping quality of the vaccine. Intrinsic thermo-stability studies revealed the rapid deterioration of the vaccine, when compared to the vaccine stabilized with the either of the stabilizers. Among the diluents used for reconstitution of the vaccine, quality in terms of infectivity titers of the virus was well preserved in vaccine diluted with 0.85% NSS compared to other diluents like PBS, DW and 1 M MgSO 4 . The 1 M MgSO 4 found unsuitable for diluting the orf vaccine. The study suggests that the LS at 25 and 45°C and LHT at 37°C are the choice of stabilizers and 0.85% NSS is the choice of diluent for orf vaccine at all temperatures contemplated.

show abstract

“…The envelope gene (B2L) of ORFV encodes a highly immunogenic envelope protein of about 42 kDa. The B2L, DNA polymerase, GIF/IL-2 and VIR genes have been used in studies related to genetic variation and molecular epidemiology [3,12,14,15].…”

mentioning

confidence: 99%

“…The B2L gene based phylogenetic analyses presented here revealed that four species of parapoxviruses (ORFV, PCPV, BPSV, PVNZ) form separate clusters with nucleotide identity levels in the range of 46-95 %. Recently there have been increased reports of CPDV infection worldwide and is enzootic in India [3,12,20]. More recently an outbreak of CPDV infection was reported in Muzzaffarnagari sheep flock of the CIRG, Makhdoom, Mathura, with about 10 % morbidity and no mortality [12].…”

mentioning

confidence: 99%

Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India

et al. 2014

View full text Add to dashboard Cite

This article describes the isolation and identification of contagious pustular dermatitis virus/orf virus (ORFV) from an outbreak of contagious pustular dermatitis (orf) in flocks of goats, in the north western region of India (Rajasthan). The virus was isolated in Vero cell cultures from scab and swab suspensions and has been identified using GIF/IL-2 and B2L gene specific primers in PCR and sequencing. The virus showed high nucleotide identity with previously reported Chinese, far eastern, Brazilian and Indian isolates. This report described the use of molecular tools for fast, reliable and confirmatory diagnosis of ORFV infection.

show abstract

Identification and phylogenetic analysis of orf viruses isolated from outbreaks in goats of Assam, a northeastern state of India

Cited by 39 publications

References 11 publications

Occurrence and identification of contagious ecthyma in blackbuck

Occurrence and identification of contagious ecthyma in blackbuck

Effect of Stabilization and Reconstitution on the Stability of a Novel Strain of Live Attenuated Orf Vaccine (ORFV MUK59/05)

Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India

Contact Info

Product

Resources

About